Tailor-made Antibodies
and Tools for Life Science

Proton ATPase antibody 116 kDa subunit - 109 002 K.D.

VOa1 is the largest subunit of the vacuolar proton pump in synaptic vesicles and coated vesicles
Rabbit polyclonal antiserum
Cat. No.: 109 002
Amount: 200 µl
Price: $360.00
Cat. No. 109 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 100 (see remarks) gallery  
IHC: not recommended
IHC-P: 1 : 200 gallery  
Immunogen Synthetic peptide corresponding to AA 826 to 838 from rat Proton ATPase (UniProt Id: P25286)
Reactivity Reacts with: rat (P25286), mouse (Q9Z1G4), hamster.
Other species not tested yet.
Specificity Specific for the α1 116kDa subunit. K.D. validated PubMed: 21795392
Matching control protein/peptide 109-0P
Remarks

WB:  Proton ATPase aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
ICC: Methanol fixation is recommended.

Data sheet 109_002.pdf

References for Proton ATPase - 109 002

Suggestion of creatine as a new neurotransmitter by approaches ranging from chemical analysis and biochemistry to electrophysiology.
Bian X, Zhu J, Jia X, Liang W, Yu S, Li Z, Zhang W, Rao Y
eLife (2023) 12: . 109 002 WB; tested species: mouse
Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2.
Kreutzberger AJB, Kiessling V, Doyle CA, Schenk N, Upchurch CM, Elmer-Dixon M, Ward AE, Preobraschenski J, Hussein SS, Tomaka W, Seelheim P, et al.
eLife (2020) 9: . 109 002 WB; tested species: rat
BAX inhibitor-1-associated V-ATPase glycosylation enhances collagen degradation in pulmonary fibrosis.
Lee MR, Lee GH, Lee HY, Kim DS, Chung MJ, Lee YC, Kim HR, Chae HJ
Cell death & disease (2014) 5: e1113. 109 002 WB; tested species: human
Loss of the SV2-like protein SVOP produces no apparent deficits in laboratory mice.
Yao J, de la Iglesia HO, Bajjalieh SM
PloS one (2013) 87: e68215. 109 002 WB; tested species: mouse
The neural cell adhesion molecule promotes maturation of the presynaptic endocytotic machinery by switching synaptic vesicle recycling from adaptor protein 3 (AP-3)- to AP-2-dependent mechanisms.
Shetty A, Sytnyk V, Leshchyns'ka I, Puchkov D, Haucke V, Schachner M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3342: 16828-45. 109 002 WB; tested species: mouse
A role for presenilins in autophagy revisited: normal acidification of lysosomes in cells lacking PSEN1 and PSEN2.
Zhang X, Garbett K, Veeraraghavalu K, Wilburn B, Gilmore R, Mirnics K, Sisodia SS
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3225: 8633-48. 109 002 WB
Vacuolar H(+)-ATPase subunits Voa1 and Voa2 cooperatively regulate secretory vesicle acidification, transmitter uptake, and storage.
Saw NM, Kang SY, Parsaud L, Han GA, Jiang T, Grzegorczyk K, Surkont M, Sun-Wada GH, Wada Y, Li L, Sugita S, et al.
Molecular biology of the cell (2011) 2218: 3394-409. 109 002 WB; KD verified; tested species: rat
Lysosomal proteolysis and autophagy require presenilin 1 and are disrupted by Alzheimer-related PS1 mutations.
Lee JH, Yu WH, Kumar A, Lee S, Mohan PS, Peterhoff CM, Wolfe DM, Martinez-Vicente M, Massey AC, Sovak G, Uchiyama Y, et al.
Cell (2010) 1417: 1146-58. 109 002 WB, ICC; tested species: mouse
Differential sorting of the vesicular glutamate transporter 1 into a defined vesicular pool is regulated by light signaling involving the clock gene Period2.
Yelamanchili SV, Pendyala G, Brunk I, Darna M, Albrecht U, Ahnert-Hilger G
The Journal of biological chemistry (2006) 28123: 15671-9. 109 002 WB; tested species: mouse
Immunoisolation of two synaptic vesicle pools from synaptosomes: a proteomics analysis.
Morciano M, Burré J, Corvey C, Karas M, Zimmermann H, Volknandt W
Journal of neurochemistry (2005) 956: 1732-45. 109 002 WB; tested species: rat
Genetic analysis of the neuronal and ubiquitous AP-3 adaptor complexes reveals divergent functions in brain.
Seong E, Wainer BH, Hughes ED, Saunders TL, Burmeister M, Faundez V
Molecular biology of the cell (2005) 161: 128-40. 109 002 WB; tested species: mouse
Tetanus toxin is transported in a novel neuronal compartment characterized by a specialized pH regulation.
Bohnert S, Schiavo G
The Journal of biological chemistry (2005) 28051: 42336-44. 109 002 WB, ICC; tested species: rat
Cat. No.: 109 002
Amount: 200 µl
Price: $360.00