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Home > Products > 123 002

NSF antibody - 123 002

NSF is a trimeric ATPase required for membrane fusion during vesicular transport
Rabbit polyclonal antiserum
Cat. No.: 123 002
Amount: 200 µl
Price: $355.00
Cat. No. 123 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 up to 1 : 1000 gallery  
IHC: 1 : 200 gallery  
IHC-P: yes
ELISA: yes (see remarks)

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Immunogen Synthetic peptide corresponding to AA 733 to 744 from rat NSF (UniProt Id: Q9QUL6)
Reactivity Reacts with: human (P46459), rat (Q9QUL6), mouse (P46460), hamster.
Other species not tested yet.
Matching control protein/peptide 123-0P
Remarks

ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 123 011 as capture antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet 123_002.pdf

References for NSF - 123 002

Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission.
Jäpel M, Gerth F, Sakaba T, Bacetic J, Yao L, Koo SJ, Maritzen T, Freund C, Haucke V
Cell reports (2020) 302: 409-420.e6. 123 002 WB; tested species: mouse
A connection between reversible tyrosine phosphorylation and SNARE complex-disassembly activity of N-ethylmaleimide-sensitive factor unveiled by the phosphomimetic mutant N-ethylmaleimide-sensitive factor-Y83E.
Ruete MC, Zarelli VEP, Masone D, Paola M, Bustos DM, Tomes CN
Molecular human reproduction (2019) : . 123 002 WB; tested species: human
Tuning of glutamate, but not GABA, release by an intra-synaptic vesicles APP domain whose function can be modulated by β- or α-secretase cleavage.
Yao W, Tambini MD, Liu X, D'Adamio L
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 123 002 WB; tested species: mouse
Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes.
de Paola M, Bello OD, Michaut MA
PloS one (2015) 108: e0135679. 123 002 WB
The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells.
Tian JH, Wu ZX, Unzicker M, Lu L, Cai Q, Li C, Schirra C, Matti U, Stevens D, Deng C, Rettig J, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2005) 2545: 10546-55. 123 002 WB
Calcium-triggered acrosomal exocytosis in human spermatozoa requires the coordinated activation of Rab3A and N-ethylmaleimide-sensitive factor.
Michaut M, Tomes CN, De Blas G, Yunes R, Mayorga LS
Proceedings of the National Academy of Sciences of the United States of America (2000) 9718: 9996-10001. 123 002 WB, ICC
Autistic-Like Behavior and Impairment of Serotonin Transporter and AMPA Receptor Trafficking in N-Ethylmaleimide Sensitive Factor Gene-Deficient Mice.
Xie MJ, Iwata K, Ishikawa Y, Nomura Y, Tani T, Murata K, Fukazawa Y, Matsuzaki H
Frontiers in genetics (2021) 12: 748627. 123 002 ICC; tested species: mouse
Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy.
Richter KN, Revelo NH, Seitz KJ, Helm MS, Sarkar D, Saleeb RS, D'Este E, Eberle J, Wagner E, Vogl C, Lazaro DF, et al.
The EMBO journal (2018) 371: 139-159. 123 002 ICC; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 123 002 ICC, IHC; tested species: mouse,rat
Constrained intracellular survival of Mycobacterium tuberculosis in human dendritic cells.
Tailleux L, Neyrolles O, Honoré-Bouakline S, Perret E, Sanchez F, Abastado JP, Lagrange PH, Gluckman JC, Rosenzwajg M, Herrmann JL
Journal of immunology (Baltimore, Md. : 1950) (2003) 1704: 1939-48. 123 002 ICC
Calcium-triggered acrosomal exocytosis in human spermatozoa requires the coordinated activation of Rab3A and N-ethylmaleimide-sensitive factor.
Michaut M, Tomes CN, De Blas G, Yunes R, Mayorga LS
Proceedings of the National Academy of Sciences of the United States of America (2000) 9718: 9996-10001. 123 002 WB, ICC
Pleiotropic effects of alpha-SNAP M105I mutation on oocyte biology: ultrastructural and cellular changes that adversely affect female fertility in mice.
de Paola M, Miró MP, Ratto M, Bátiz LF, Michaut MA
Scientific reports (2019) 91: 17374. 123 002 IHC; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 123 002 ICC, IHC; tested species: mouse,rat
Cat. No.: 123 002
Amount: 200 µl
Price: $355.00
Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission.
Jäpel M, Gerth F, Sakaba T, Bacetic J, Yao L, Koo SJ, Maritzen T, Freund C, Haucke V
Cell reports (2020) 302: 409-420.e6. 123 002 WB; tested species: mouse
A connection between reversible tyrosine phosphorylation and SNARE complex-disassembly activity of N-ethylmaleimide-sensitive factor unveiled by the phosphomimetic mutant N-ethylmaleimide-sensitive factor-Y83E.
Ruete MC, Zarelli VEP, Masone D, Paola M, Bustos DM, Tomes CN
Molecular human reproduction (2019) : . 123 002 WB; tested species: human
Tuning of glutamate, but not GABA, release by an intra-synaptic vesicles APP domain whose function can be modulated by β- or α-secretase cleavage.
Yao W, Tambini MD, Liu X, D'Adamio L
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 123 002 WB; tested species: mouse
Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes.
de Paola M, Bello OD, Michaut MA
PloS one (2015) 108: e0135679. 123 002 WB
The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells.
Tian JH, Wu ZX, Unzicker M, Lu L, Cai Q, Li C, Schirra C, Matti U, Stevens D, Deng C, Rettig J, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2005) 2545: 10546-55. 123 002 WB
Calcium-triggered acrosomal exocytosis in human spermatozoa requires the coordinated activation of Rab3A and N-ethylmaleimide-sensitive factor.
Michaut M, Tomes CN, De Blas G, Yunes R, Mayorga LS
Proceedings of the National Academy of Sciences of the United States of America (2000) 9718: 9996-10001. 123 002 WB, ICC
Autistic-Like Behavior and Impairment of Serotonin Transporter and AMPA Receptor Trafficking in N-Ethylmaleimide Sensitive Factor Gene-Deficient Mice.
Xie MJ, Iwata K, Ishikawa Y, Nomura Y, Tani T, Murata K, Fukazawa Y, Matsuzaki H
Frontiers in genetics (2021) 12: 748627. 123 002 ICC; tested species: mouse
Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy.
Richter KN, Revelo NH, Seitz KJ, Helm MS, Sarkar D, Saleeb RS, D'Este E, Eberle J, Wagner E, Vogl C, Lazaro DF, et al.
The EMBO journal (2018) 371: 139-159. 123 002 ICC; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 123 002 ICC, IHC; tested species: mouse,rat
Constrained intracellular survival of Mycobacterium tuberculosis in human dendritic cells.
Tailleux L, Neyrolles O, Honoré-Bouakline S, Perret E, Sanchez F, Abastado JP, Lagrange PH, Gluckman JC, Rosenzwajg M, Herrmann JL
Journal of immunology (Baltimore, Md. : 1950) (2003) 1704: 1939-48. 123 002 ICC
Calcium-triggered acrosomal exocytosis in human spermatozoa requires the coordinated activation of Rab3A and N-ethylmaleimide-sensitive factor.
Michaut M, Tomes CN, De Blas G, Yunes R, Mayorga LS
Proceedings of the National Academy of Sciences of the United States of America (2000) 9718: 9996-10001. 123 002 WB, ICC
Pleiotropic effects of alpha-SNAP M105I mutation on oocyte biology: ultrastructural and cellular changes that adversely affect female fertility in mice.
de Paola M, Miró MP, Ratto M, Bátiz LF, Michaut MA
Scientific reports (2019) 91: 17374. 123 002 IHC; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 123 002 ICC, IHC; tested species: mouse,rat
WB
ICC
IHC
Background

N-ethylamide sensitive fusion protein NSF functions together with SNAPs (soluble NSF attachment proteins) and SNAREs (SNAP receptors) in vesicular transport.
NSF is a homotrimer whose polypeptide subunits are made up of three distinct domains: an amino - terminal domain (N) and two homologous ATP-binding domains (D1 and D2). NSF is an ATPase that dissociates SNARE complexes, such as the core complex composed of synaptobrevin/VAMP, syntaxin 1 and SNAP 25 under ATP hydrolysis. The ability of the D1 domain to hydrolyze ATP is required for NSF activity. The D2 domain is required for trimerization, but its ability to hydrolyze ATP is not absolutely required for NSF function.
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