Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 129 011

Neuroligin1/2/3/4 antibody - 129 011 K.O.

Neuroligins are postsynaptic cell adhesion molecules
Mouse monoclonal purified IgG
Cat. No.: 129 011
Amount: 100 µg
Price: $415.00
Cat. No. 129 011 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: not recommended
IHC: not recommended
IHC-P: not tested yet
ELISA: yes (see remarks)

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Clone 4F9
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 695 from rat Neuroligin1 (UniProt Id: Q62765)
Reactivity Reacts with: rat (Q62765, Q62888, Q62889, ), mouse (Q99K10, Q69ZK9, Q8BYM5, B0F2B4).
Other species not tested yet.
Specificity Recognizes rat and mouse neuroligins 1-3 and mouse neuroligin 4. K.O. validated
Remarks

ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 129 103 or 129 203 as detector antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet 129_011.pdf

References for Neuroligin1/2/3/4 - 129 011

Truncated tau deregulates synaptic markers in rat model for human tauopathy.
Jadhav S, Katina S, Kovac A, Kazmerova Z, Novak M, Zilka N
Frontiers in cellular neuroscience (2015) 9: 24. 129 011 WB
The association of GPR85 with PSD-95-neuroligin complex and autism spectrum disorder: a molecular analysis.
Fujita-Jimbo E, Tanabe Y, Yu Z, Kojima K, Mori M, Li H, Iwamoto S, Yamagata T, Momoi MY, Momoi T
Molecular autism (2015) 6: 17. 129 011 WB
Neuroligin 1 is dynamically exchanged at postsynaptic sites.
Schapitz IU, Behrend B, Pechmann Y, Lappe-Siefke C, Kneussel SJ, Wallace KE, Stempel AV, Buck F, Grant SG, Schweizer M, Schmitz D, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3038: 12733-44. 129 011 WB; tested species: rat
The synaptic proteins neurexins and neuroligins are widely expressed in the vascular system and contribute to its functions.
Bottos A, Destro E, Rissone A, Graziano S, Cordara G, Assenzio B, Cera MR, Mascia L, Bussolino F, Arese M
Proceedings of the National Academy of Sciences of the United States of America (2009) 10649: 20782-7. 129 011 IHC, WB, IP; tested species: chicken
Postsynaptic neuroligin enhances presynaptic inputs at neuronal nicotinic synapses.
Conroy WG, Nai Q, Ross B, Naughton G, Berg DK
Developmental biology (2007) 3071: 79-91. 129 011 WB
A mutation linked with autism reveals a common mechanism of endoplasmic reticulum retention for the alpha,beta-hydrolase fold protein family.
De Jaco A, Comoletti D, Kovarik Z, Gaietta G, Radic Z, Lockridge O, Ellisman MH, Taylor P
The Journal of biological chemistry (2006) 28114: 9667-76. 129 011 WB
Control of excitatory and inhibitory synapse formation by neuroligins.
Chih B, Engelman H, Scheiffele P
Science (New York, N.Y.) (2005) 3075713: 1324-8. 129 011 WB
The synaptic proteins neurexins and neuroligins are widely expressed in the vascular system and contribute to its functions.
Bottos A, Destro E, Rissone A, Graziano S, Cordara G, Assenzio B, Cera MR, Mascia L, Bussolino F, Arese M
Proceedings of the National Academy of Sciences of the United States of America (2009) 10649: 20782-7. 129 011 IHC, WB, IP; tested species: chicken
The synaptic proteins neurexins and neuroligins are widely expressed in the vascular system and contribute to its functions.
Bottos A, Destro E, Rissone A, Graziano S, Cordara G, Assenzio B, Cera MR, Mascia L, Bussolino F, Arese M
Proceedings of the National Academy of Sciences of the United States of America (2009) 10649: 20782-7. 129 011 IHC, WB, IP; tested species: chicken
Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.
Vasileva M, Horstmann H, Geumann C, Gitler D, Kuner T
The European journal of neuroscience (2012) 368: 3005-20. 129 011 ELISA
Cat. No.: 129 011
Amount: 100 µg
Price: $415.00
Truncated tau deregulates synaptic markers in rat model for human tauopathy.
Jadhav S, Katina S, Kovac A, Kazmerova Z, Novak M, Zilka N
Frontiers in cellular neuroscience (2015) 9: 24. 129 011 WB
The association of GPR85 with PSD-95-neuroligin complex and autism spectrum disorder: a molecular analysis.
Fujita-Jimbo E, Tanabe Y, Yu Z, Kojima K, Mori M, Li H, Iwamoto S, Yamagata T, Momoi MY, Momoi T
Molecular autism (2015) 6: 17. 129 011 WB
Neuroligin 1 is dynamically exchanged at postsynaptic sites.
Schapitz IU, Behrend B, Pechmann Y, Lappe-Siefke C, Kneussel SJ, Wallace KE, Stempel AV, Buck F, Grant SG, Schweizer M, Schmitz D, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3038: 12733-44. 129 011 WB; tested species: rat
The synaptic proteins neurexins and neuroligins are widely expressed in the vascular system and contribute to its functions.
Bottos A, Destro E, Rissone A, Graziano S, Cordara G, Assenzio B, Cera MR, Mascia L, Bussolino F, Arese M
Proceedings of the National Academy of Sciences of the United States of America (2009) 10649: 20782-7. 129 011 IHC, WB, IP; tested species: chicken
Postsynaptic neuroligin enhances presynaptic inputs at neuronal nicotinic synapses.
Conroy WG, Nai Q, Ross B, Naughton G, Berg DK
Developmental biology (2007) 3071: 79-91. 129 011 WB
A mutation linked with autism reveals a common mechanism of endoplasmic reticulum retention for the alpha,beta-hydrolase fold protein family.
De Jaco A, Comoletti D, Kovarik Z, Gaietta G, Radic Z, Lockridge O, Ellisman MH, Taylor P
The Journal of biological chemistry (2006) 28114: 9667-76. 129 011 WB
Control of excitatory and inhibitory synapse formation by neuroligins.
Chih B, Engelman H, Scheiffele P
Science (New York, N.Y.) (2005) 3075713: 1324-8. 129 011 WB
The synaptic proteins neurexins and neuroligins are widely expressed in the vascular system and contribute to its functions.
Bottos A, Destro E, Rissone A, Graziano S, Cordara G, Assenzio B, Cera MR, Mascia L, Bussolino F, Arese M
Proceedings of the National Academy of Sciences of the United States of America (2009) 10649: 20782-7. 129 011 IHC, WB, IP; tested species: chicken
The synaptic proteins neurexins and neuroligins are widely expressed in the vascular system and contribute to its functions.
Bottos A, Destro E, Rissone A, Graziano S, Cordara G, Assenzio B, Cera MR, Mascia L, Bussolino F, Arese M
Proceedings of the National Academy of Sciences of the United States of America (2009) 10649: 20782-7. 129 011 IHC, WB, IP; tested species: chicken
Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.
Vasileva M, Horstmann H, Geumann C, Gitler D, Kuner T
The European journal of neuroscience (2012) 368: 3005-20. 129 011 ELISA
WB
Background

Neuroligins form a family of postsynaptic cell surface molecules that interact with β-neurexins. They are 110-120 kDa polypeptides with homology to acetylcholine esterase. Neuroligin1 and neuroligin3 are specifically localized to post-synaptic densities of excitatory synapses whereas neuroligin2 is found exclusively on inhibitory synapses.
Mutations in neuroligin3 and neuroligin4 have been implicated with a rare, heritable form of autism.
Other products for this target