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Home > Products > 219 017

NCAM180 antibody - 219 017

NCAM180 is a neural cell adhesion molecule
Rat monoclonal purified IgG
Cat. No.: 219 017
Amount: 100 µg
Price: $415.00
Cat. No. 219 017 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 200 up to 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: not tested yet
EM: yes
Clone 481
Subtype IgG
Immunogen Recombinant protein corresponding to AA 1 to 1115 from mouse NCAM180 (UniProt Id: P13595)
Epitop Antibody binds to the intracellular C-terminus of NCAM180
Reactivity Reacts with: rat (P13596), mouse (P13595).
Other species not tested yet.
Specificity Specific for NCAM180. Does not detect NCAM120 and NCAM140.
Remarks

This antibody lacks the Fc region and has to be used like a Fab fragment. Secondary reagents directed exclusively against the Fc region are not recommended.
Data sheet 219_017.pdf

References for NCAM180 - 219 017

Developmentally regulated masking of an intracellular epitope of the 180 kDa isoform of the neural cell adhesion molecule NCAM.
Kramer I, Hall H, Bleistein U, Schachner M
Journal of neuroscience research (1997) 492: 161-75. 219 017 WB, ICC, IHC
Selective expression of the 180-kD component of the neural cell adhesion molecule N-CAM during development.
Pollerberg EG, Sadoul R, Goridis C, Schachner M
The Journal of cell biology (1985) 1015 Pt 1: 1921-9. 219 017 WB, ICC, IHC
A multimodal 3D neuro-microphysiological system with neurite-trapping microelectrodes.
Molina-Martínez B, Jentsch LV, Ersoy F, van der Moolen M, Donato S, Ness TV, Heutink P, Jones PD, Cesare P
Biofabrication (2022) 142: . 219 017 ICC; tested species: human
Developmentally regulated masking of an intracellular epitope of the 180 kDa isoform of the neural cell adhesion molecule NCAM.
Kramer I, Hall H, Bleistein U, Schachner M
Journal of neuroscience research (1997) 492: 161-75. 219 017 WB, ICC, IHC
Selective expression of the 180-kD component of the neural cell adhesion molecule N-CAM during development.
Pollerberg EG, Sadoul R, Goridis C, Schachner M
The Journal of cell biology (1985) 1015 Pt 1: 1921-9. 219 017 WB, ICC, IHC
Immunoelectron microscopic localization of the neural recognition molecules L1, NCAM, and its isoform NCAM180, the NCAM-associated polysialic acid, beta1 integrin and the extracellular matrix molecule tenascin-R in synapses of the adult rat hippocampus.
Schuster T, Krug M, Stalder M, Hackel N, Gerardy-Schahn R, Schachner M
Journal of neurobiology (2001) 492: 142-58. 219 017 EM, IHC
Developmentally regulated masking of an intracellular epitope of the 180 kDa isoform of the neural cell adhesion molecule NCAM.
Kramer I, Hall H, Bleistein U, Schachner M
Journal of neuroscience research (1997) 492: 161-75. 219 017 WB, ICC, IHC
Selective expression of the 180-kD component of the neural cell adhesion molecule N-CAM during development.
Pollerberg EG, Sadoul R, Goridis C, Schachner M
The Journal of cell biology (1985) 1015 Pt 1: 1921-9. 219 017 WB, ICC, IHC
Immunoelectron microscopic localization of the neural recognition molecules L1, NCAM, and its isoform NCAM180, the NCAM-associated polysialic acid, beta1 integrin and the extracellular matrix molecule tenascin-R in synapses of the adult rat hippocampus.
Schuster T, Krug M, Stalder M, Hackel N, Gerardy-Schahn R, Schachner M
Journal of neurobiology (2001) 492: 142-58. 219 017 EM, IHC
Cat. No.: 219 017
Amount: 100 µg
Price: $415.00
Developmentally regulated masking of an intracellular epitope of the 180 kDa isoform of the neural cell adhesion molecule NCAM.
Kramer I, Hall H, Bleistein U, Schachner M
Journal of neuroscience research (1997) 492: 161-75. 219 017 WB, ICC, IHC
Selective expression of the 180-kD component of the neural cell adhesion molecule N-CAM during development.
Pollerberg EG, Sadoul R, Goridis C, Schachner M
The Journal of cell biology (1985) 1015 Pt 1: 1921-9. 219 017 WB, ICC, IHC
A multimodal 3D neuro-microphysiological system with neurite-trapping microelectrodes.
Molina-Martínez B, Jentsch LV, Ersoy F, van der Moolen M, Donato S, Ness TV, Heutink P, Jones PD, Cesare P
Biofabrication (2022) 142: . 219 017 ICC; tested species: human
Developmentally regulated masking of an intracellular epitope of the 180 kDa isoform of the neural cell adhesion molecule NCAM.
Kramer I, Hall H, Bleistein U, Schachner M
Journal of neuroscience research (1997) 492: 161-75. 219 017 WB, ICC, IHC
Selective expression of the 180-kD component of the neural cell adhesion molecule N-CAM during development.
Pollerberg EG, Sadoul R, Goridis C, Schachner M
The Journal of cell biology (1985) 1015 Pt 1: 1921-9. 219 017 WB, ICC, IHC
Immunoelectron microscopic localization of the neural recognition molecules L1, NCAM, and its isoform NCAM180, the NCAM-associated polysialic acid, beta1 integrin and the extracellular matrix molecule tenascin-R in synapses of the adult rat hippocampus.
Schuster T, Krug M, Stalder M, Hackel N, Gerardy-Schahn R, Schachner M
Journal of neurobiology (2001) 492: 142-58. 219 017 EM, IHC
Developmentally regulated masking of an intracellular epitope of the 180 kDa isoform of the neural cell adhesion molecule NCAM.
Kramer I, Hall H, Bleistein U, Schachner M
Journal of neuroscience research (1997) 492: 161-75. 219 017 WB, ICC, IHC
Selective expression of the 180-kD component of the neural cell adhesion molecule N-CAM during development.
Pollerberg EG, Sadoul R, Goridis C, Schachner M
The Journal of cell biology (1985) 1015 Pt 1: 1921-9. 219 017 WB, ICC, IHC
Immunoelectron microscopic localization of the neural recognition molecules L1, NCAM, and its isoform NCAM180, the NCAM-associated polysialic acid, beta1 integrin and the extracellular matrix molecule tenascin-R in synapses of the adult rat hippocampus.
Schuster T, Krug M, Stalder M, Hackel N, Gerardy-Schahn R, Schachner M
Journal of neurobiology (2001) 492: 142-58. 219 017 EM, IHC
WB
ICC
IHC
Background
The neural cell adhesion molecule (NCAM) is a cell surface glycoprotein that occurs in several isoforms. It belongs to the immunglobulin superfamily of adhesion molecules and contains several immunglobulin-like domains. The largest, NCAM 180, may play an important role in the development and stabilization of cell contacts. It has been shown to accumulate at those sites of contact and at postsynaptic densities.