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Home > Products > 116 002

Munc18-1 antibody - 116 002 K.O.

Munc18 proteins are peripheral membrane protein involved in vesicle docking and fusion
Rabbit polyclonal antiserum
Cat. No.: 116 002
Amount: 200 µl
Price: $355.00
Cat. No. 116 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
ELISA: yes (see remarks)

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Immunogen Synthetic peptide corresponding to AA 580 to 594 from rat Munc18-1 (UniProt Id: P61765)
Reactivity Reacts with: human (P61764), rat (P61765), mouse (O08599), cow.
Other species not tested yet.
Specificity K.O. validated PubMed: 32073399
Matching control protein/peptide 116-0P
Remarks

ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 116 011 as capture antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet 116_002.pdf

References for Munc18-1 - 116 002

GABAergic/Glycinergic and Glutamatergic Neurons Mediate Distinct Neurodevelopmental Phenotypes of STXBP1 Encephalopathy.
Kim JH, Chen W, Chao ES, Rivera A, Kaku HN, Jiang K, Lee D, Chen H, Vega JM, Chin TV, Jin K, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2024) 4414: . 116 002 WB; tested species: mouse
Reduced synaptic depression in human neurons carrying homozygous disease-causing STXBP1 variant L446F.
Öttl M, Toonen RF, Verhage M
Human molecular genetics (2024) : . 116 002 WB; tested species: stem cells
A synthetic organelle approach to probe SNARE-mediated membrane fusion in a bacterial host.
Ferreras S, Singh NP, Le Borgne R, Bun P, Binz T, Parton RG, Verbavatz JM, Vannier C, Galli T
The Journal of biological chemistry (2023) 2993: 102974. 116 002 WB; tested species: mouse
A novel synaptopathy-defective synaptic vesicle protein trafficking in the mutant CHMP2B mouse model of frontotemporal dementia.
Clayton EL, Bonnycastle K, Isaacs AM, Cousin MA, Schorge S
Journal of neurochemistry (2022) 1603: 412-425. 116 002 WB; tested species: mouse
MAFA and MAFB regulate exocytosis-related genes in human β-cells.
Cataldo LR, Singh T, Achanta K, Bsharat S, Prasad RB, Luan C, Renström E, Eliasson L, Artner I
Acta physiologica (Oxford, England) (2022) 2342: e13761. 116 002 WB, IHC; tested species: mouse
Munc13-1 is a Ca2+-phospholipid-dependent vesicle priming hub that shapes synaptic short-term plasticity and enables sustained neurotransmission.
Lipstein N, Chang S, Lin KH, López-Murcia FJ, Neher E, Taschenberger H, Brose N
Neuron (2021) : . 116 002 WB; tested species: mouse
Stxbp1/Munc18-1 haploinsufficiency impairs inhibition and mediates key neurological features of STXBP1 encephalopathy.
Chen W, Cai ZL, Chao ES, Chen H, Longley CM, Hao S, Chao HT, Kim JH, Messier JE, Zoghbi HY, Tang J, et al.
eLife (2020) 9: . 116 002 WB; KO verified; tested species: mouse
Potential Protection Against Type 2 Diabetes in Obesity Through Lower CD36 Expression and Improved Exocytosis in β-Cells.
Nagao M, Esguerra JLS, Asai A, Ofori JK, Edlund A, Wendt A, Sugihara H, Wollheim CB, Oikawa S, Eliasson L
Diabetes (2020) 696: 1193-1205. 116 002 WB; tested species: human
Endophilin-A coordinates priming and fusion of neurosecretory vesicles via intersectin.
Gowrisankaran S, Houy S, Del Castillo JGP, Steubler V, Gelker M, Kroll J, Pinheiro PS, Schwitters D, Halbsgut N, Pechstein A, van Weering JRT, et al.
Nature communications (2020) 111: 1266. 116 002 WB, ICC; tested species: mouse
Autism and Schizophrenia-Associated CYFIP1 Regulates the Balance of Synaptic Excitation and Inhibition.
Davenport EC, Szulc BR, Drew J, Taylor J, Morgan T, Higgs NF, López-Doménech G, Kittler JT
Cell reports (2019) 268: 2037-2051.e6. 116 002 WB, ICC; tested species: mouse
Tuning of glutamate, but not GABA, release by an intra-synaptic vesicles APP domain whose function can be modulated by β- or α-secretase cleavage.
Yao W, Tambini MD, Liu X, D'Adamio L
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 116 002 WB; tested species: mouse
Merlin modulates process outgrowth and synaptogenesis in the cerebellum.
Toledo A, Lang F, Doengi M, Morrison H, Stein V, Baader SL
Brain structure & function (2019) : . 116 002 WB; tested species: mouse
Dysregulation of the SNARE-binding protein Munc18-1 impairs BDNF secretion and synaptic neurotransmission: a novel interventional target to protect the aging brain.
Lee YI, Kim YG, Pyeon HJ, Ahn JC, Logan S, Orock A, Joo KM, Lőrincz A, Deák F
GeroScience (2019) : . 116 002 WB, IHC; KD verified; tested species: mouse
Munc18-1 haploinsufficiency impairs learning and memory by reduced synaptic vesicular release in a model of Ohtahara syndrome.
Orock A, Logan S, Deak F
Molecular and cellular neurosciences (2018) 88: 33-42. 116 002 WB; KD verified; tested species: mouse
Overexpression of miR-1 in the heart attenuates hippocampal synaptic vesicle exocytosis by the posttranscriptional regulation of SNAP-25 through the transportation of exosomes.
Duan MJ, Yan ML, Wang Q, Mao M, Su D, Sun LL, Li KX, Qu Y, Sun Q, Zhang XY, Huang SY, et al.
Cell communication and signaling : CCS (2018) 161: 91. 116 002 WB; tested species: mouse
SNAP-25a and SNAP-25b differently mediate interactions with Munc18-1 and Gβγ subunits.
Daraio T, Valladolid-Acebes I, Brismar K, Bark C
Neuroscience letters (2018) 674: 75-80. 116 002 WB; tested species: mouse
MiR-335 overexpression impairs insulin secretion through defective priming of insulin vesicles.
Salunkhe VA, Ofori JK, Gandasi NR, Salö SA, Hansson S, Andersson ME, Wendt A, Barg S, Esguerra JLS, Eliasson L
Physiological reports (2017) 521: . 116 002 WB; tested species: rat
Potentiation of excitatory synaptic transmission ameliorates aggression in mice with Stxbp1 haploinsufficiency.
Miyamoto H, Shimohata A, Abe M, Abe T, Mazaki E, Amano K, Suzuki T, Tatsukawa T, Itohara S, Sakimura K, Yamakawa K, et al.
Human molecular genetics (2017) 2624: 4961-4974. 116 002 WB; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 116 002 WB
Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission.
Spangler SA, Schmitz SK, Kevenaar JT, de Graaff E, de Wit H, Demmers J, Toonen RF, Hoogenraad CC
The Journal of cell biology (2013) 2016: 915-28. 116 002 WB, ICC
mSYD1A, a mammalian synapse-defective-1 protein, regulates synaptogenic signaling and vesicle docking.
Wentzel C, Sommer JE, Nair R, Stiefvater A, Sibarita JB, Scheiffele P
Neuron (2013) 786: 1012-23. 116 002 WB; tested species: mouse
Role for Reelin in neurotransmitter release.
Hellwig S, Hack I, Kowalski J, Brunne B, Jarowyj J, Unger A, Bock HH, Junghans D, Frotscher M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 317: 2352-60. 116 002 WB
Abrogating Munc18-1-SNARE complex interaction has limited impact on exocytosis in PC12 cells.
Malintan NT, Nguyen TH, Han L, Latham CF, Osborne SL, Wen PJ, Lim SJ, Sugita S, Collins BM, Meunier FA
The Journal of biological chemistry (2009) 28432: 21637-46. 116 002 WB, ICC; tested species: rat
Preferential increase in the hippocampal synaptic vesicle protein 2A (SV2A) by pentylenetetrazole kindling.
Ohno Y, Ishihara S, Terada R, Kikuta M, Sofue N, Kawai Y, Serikawa T, Sasa M
Biochemical and biophysical research communications (2009) 3903: 415-20. 116 002 WB
Synaptic vesicle docking: sphingosine regulates syntaxin1 interaction with Munc18.
Camoletto PG, Vara H, Morando L, Connell E, Marletto FP, Giustetto M, Sassoè-Pognetto M, Van Veldhoven PP, Ledesma MD
PloS one (2009) 44: e5310. 116 002 WB
Proteomics of photoreceptor outer segments identifies a subset of SNARE and Rab proteins implicated in membrane vesicle trafficking and fusion.
Kwok MC, Holopainen JM, Molday LL, Foster LJ, Molday RS
Molecular & cellular proteomics : MCP (2008) 76: 1053-66. 116 002 WB
Neurotransmitter release regulated by a MALS-liprin-alpha presynaptic complex.
Olsen O, Moore KA, Fukata M, Kazuta T, Trinidad JC, Kauer FW, Streuli M, Misawa H, Burlingame AL, Nicoll RA, Bredt DS, et al.
The Journal of cell biology (2005) 1707: 1127-34. 116 002 WB
Granuphilin molecularly docks insulin granules to the fusion machinery.
Gomi H, Mizutani S, Kasai K, Itohara S, Izumi T
The Journal of cell biology (2005) 1711: 99-109. 116 002 WB
Munc18-syntaxin complexes and exocytosis in human platelets.
Houng A, Polgar J, Reed GL
The Journal of biological chemistry (2003) 27822: 19627-33. 116 002 WB
Rab27a mediates the tight docking of insulin granules onto the plasma membrane during glucose stimulation.
Kasai K, Ohara-Imaizumi M, Takahashi N, Mizutani S, Zhao S, Kikuta T, Kasai H, Nagamatsu S, Gomi H, Izumi T
The Journal of clinical investigation (2005) 1152: 388-96. 116 002 IP
Munc-18 associates with syntaxin and serves as a negative regulator of exocytosis in the pancreatic beta -cell.
Zhang W, Efanov A, Yang SN, Fried G, Kolare S, Brown H, Zaitsev S, Berggren PO, Meister B
The Journal of biological chemistry (2000) 27552: 41521-7. 116 002 IP, IHC
A density-based enrichment measure for assessing colocalization in single-molecule localization microscopy data.
Ejdrup AL, Lycas MD, Lorenzen N, Konomi A, Herborg F, Madsen KL, Gether U
Nature communications (2022) 131: 4388. 116 002 ICC; tested species: rat
Endophilin-A coordinates priming and fusion of neurosecretory vesicles via intersectin.
Gowrisankaran S, Houy S, Del Castillo JGP, Steubler V, Gelker M, Kroll J, Pinheiro PS, Schwitters D, Halbsgut N, Pechstein A, van Weering JRT, et al.
Nature communications (2020) 111: 1266. 116 002 WB, ICC; tested species: mouse
Autism and Schizophrenia-Associated CYFIP1 Regulates the Balance of Synaptic Excitation and Inhibition.
Davenport EC, Szulc BR, Drew J, Taylor J, Morgan T, Higgs NF, López-Doménech G, Kittler JT
Cell reports (2019) 268: 2037-2051.e6. 116 002 WB, ICC; tested species: mouse
Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission.
Spangler SA, Schmitz SK, Kevenaar JT, de Graaff E, de Wit H, Demmers J, Toonen RF, Hoogenraad CC
The Journal of cell biology (2013) 2016: 915-28. 116 002 WB, ICC
Munc18-1 controls SNARE protein complex assembly during human sperm acrosomal exocytosis.
Rodríguez F, Zanetti MN, Mayorga LS, Tomes CN
The Journal of biological chemistry (2012) 28752: 43825-39. 116 002 ICC; tested species: human
Munc18-1 tuning of vesicle merger and fusion pore properties.
Jorgacevski J, Potokar M, Grilc S, Kreft M, Liu W, Barclay JW, Bückers J, Medda R, Hell SW, Parpura V, Burgoyne RD, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3124: 9055-66. 116 002 ICC
Abrogating Munc18-1-SNARE complex interaction has limited impact on exocytosis in PC12 cells.
Malintan NT, Nguyen TH, Han L, Latham CF, Osborne SL, Wen PJ, Lim SJ, Sugita S, Collins BM, Meunier FA
The Journal of biological chemistry (2009) 28432: 21637-46. 116 002 WB, ICC; tested species: rat
Fusion-related release of glutamate from astrocytes.
Zhang Q, Pangrsic T, Kreft M, Krzan M, Li N, Sul JY, Halassa M, Van Bockstaele E, Zorec R, Haydon PG
The Journal of biological chemistry (2004) 27913: 12724-33. 116 002 ICC
Lifelong absence of microglia alters hippocampal glutamatergic networks but not synapse and spine density.
Surala M, Soso-Zdravkovic L, Munro D, Rifat A, Ouk K, Vida I, Priller J, Madry C
EMBO reports (2024) : . 116 002 IHC; tested species: mouse
Proteomic analysis reveals the composition of glutamatergic organelles of auditory inner hair cell.
Cepeda AP, Ninov M, Neef J, Parfentev I, Kusch K, Reisinger E, Jahn R, Moser T, Urlaub H
Molecular & cellular proteomics : MCP (2023) : 100704. 116 002 IHC; tested species: mouse
MAFA and MAFB regulate exocytosis-related genes in human β-cells.
Cataldo LR, Singh T, Achanta K, Bsharat S, Prasad RB, Luan C, Renström E, Eliasson L, Artner I
Acta physiologica (Oxford, England) (2022) 2342: e13761. 116 002 WB, IHC; tested species: mouse
Dysregulation of the SNARE-binding protein Munc18-1 impairs BDNF secretion and synaptic neurotransmission: a novel interventional target to protect the aging brain.
Lee YI, Kim YG, Pyeon HJ, Ahn JC, Logan S, Orock A, Joo KM, Lőrincz A, Deák F
GeroScience (2019) : . 116 002 WB, IHC; KD verified; tested species: mouse
Munc18-1 redistributes in nerve terminals in an activity- and PKC-dependent manner.
Cijsouw T, Weber JP, Broeke JH, Broek JA, Schut D, Kroon T, Saarloos I, Verhage M, Toonen RF
The Journal of cell biology (2014) 2045: 759-75. 116 002 IHC
Munc-18 associates with syntaxin and serves as a negative regulator of exocytosis in the pancreatic beta -cell.
Zhang W, Efanov A, Yang SN, Fried G, Kolare S, Brown H, Zaitsev S, Berggren PO, Meister B
The Journal of biological chemistry (2000) 27552: 41521-7. 116 002 IP, IHC
Membrane Location of Syntaxin-Binding Protein 1 Is Correlated with Poor Prognosis of Lung Adenocarcinoma.
Wang X, Fu G, Wen J, Chen H, Zhang B, Zhu D
The Tohoku journal of experimental medicine (2020) 2504: 263-270. 116 002 IHC-P; tested species: human
Total arrest of spontaneous and evoked synaptic transmission but normal synaptogenesis in the absence of Munc13-mediated vesicle priming.
Varoqueaux F, Sigler A, Rhee JS, Brose N, Enk C, Reim K, Rosenmund C
Proceedings of the National Academy of Sciences of the United States of America (2002) 9913: 9037-42. 116 002
Cat. No.: 116 002
Amount: 200 µl
Price: $355.00
GABAergic/Glycinergic and Glutamatergic Neurons Mediate Distinct Neurodevelopmental Phenotypes of STXBP1 Encephalopathy.
Kim JH, Chen W, Chao ES, Rivera A, Kaku HN, Jiang K, Lee D, Chen H, Vega JM, Chin TV, Jin K, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2024) 4414: . 116 002 WB; tested species: mouse
Reduced synaptic depression in human neurons carrying homozygous disease-causing STXBP1 variant L446F.
Öttl M, Toonen RF, Verhage M
Human molecular genetics (2024) : . 116 002 WB; tested species: stem cells
A synthetic organelle approach to probe SNARE-mediated membrane fusion in a bacterial host.
Ferreras S, Singh NP, Le Borgne R, Bun P, Binz T, Parton RG, Verbavatz JM, Vannier C, Galli T
The Journal of biological chemistry (2023) 2993: 102974. 116 002 WB; tested species: mouse
A novel synaptopathy-defective synaptic vesicle protein trafficking in the mutant CHMP2B mouse model of frontotemporal dementia.
Clayton EL, Bonnycastle K, Isaacs AM, Cousin MA, Schorge S
Journal of neurochemistry (2022) 1603: 412-425. 116 002 WB; tested species: mouse
MAFA and MAFB regulate exocytosis-related genes in human β-cells.
Cataldo LR, Singh T, Achanta K, Bsharat S, Prasad RB, Luan C, Renström E, Eliasson L, Artner I
Acta physiologica (Oxford, England) (2022) 2342: e13761. 116 002 WB, IHC; tested species: mouse
Munc13-1 is a Ca2+-phospholipid-dependent vesicle priming hub that shapes synaptic short-term plasticity and enables sustained neurotransmission.
Lipstein N, Chang S, Lin KH, López-Murcia FJ, Neher E, Taschenberger H, Brose N
Neuron (2021) : . 116 002 WB; tested species: mouse
Stxbp1/Munc18-1 haploinsufficiency impairs inhibition and mediates key neurological features of STXBP1 encephalopathy.
Chen W, Cai ZL, Chao ES, Chen H, Longley CM, Hao S, Chao HT, Kim JH, Messier JE, Zoghbi HY, Tang J, et al.
eLife (2020) 9: . 116 002 WB; KO verified; tested species: mouse
Potential Protection Against Type 2 Diabetes in Obesity Through Lower CD36 Expression and Improved Exocytosis in β-Cells.
Nagao M, Esguerra JLS, Asai A, Ofori JK, Edlund A, Wendt A, Sugihara H, Wollheim CB, Oikawa S, Eliasson L
Diabetes (2020) 696: 1193-1205. 116 002 WB; tested species: human
Endophilin-A coordinates priming and fusion of neurosecretory vesicles via intersectin.
Gowrisankaran S, Houy S, Del Castillo JGP, Steubler V, Gelker M, Kroll J, Pinheiro PS, Schwitters D, Halbsgut N, Pechstein A, van Weering JRT, et al.
Nature communications (2020) 111: 1266. 116 002 WB, ICC; tested species: mouse
Autism and Schizophrenia-Associated CYFIP1 Regulates the Balance of Synaptic Excitation and Inhibition.
Davenport EC, Szulc BR, Drew J, Taylor J, Morgan T, Higgs NF, López-Doménech G, Kittler JT
Cell reports (2019) 268: 2037-2051.e6. 116 002 WB, ICC; tested species: mouse
Tuning of glutamate, but not GABA, release by an intra-synaptic vesicles APP domain whose function can be modulated by β- or α-secretase cleavage.
Yao W, Tambini MD, Liu X, D'Adamio L
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 116 002 WB; tested species: mouse
Merlin modulates process outgrowth and synaptogenesis in the cerebellum.
Toledo A, Lang F, Doengi M, Morrison H, Stein V, Baader SL
Brain structure & function (2019) : . 116 002 WB; tested species: mouse
Dysregulation of the SNARE-binding protein Munc18-1 impairs BDNF secretion and synaptic neurotransmission: a novel interventional target to protect the aging brain.
Lee YI, Kim YG, Pyeon HJ, Ahn JC, Logan S, Orock A, Joo KM, Lőrincz A, Deák F
GeroScience (2019) : . 116 002 WB, IHC; KD verified; tested species: mouse
Munc18-1 haploinsufficiency impairs learning and memory by reduced synaptic vesicular release in a model of Ohtahara syndrome.
Orock A, Logan S, Deak F
Molecular and cellular neurosciences (2018) 88: 33-42. 116 002 WB; KD verified; tested species: mouse
Overexpression of miR-1 in the heart attenuates hippocampal synaptic vesicle exocytosis by the posttranscriptional regulation of SNAP-25 through the transportation of exosomes.
Duan MJ, Yan ML, Wang Q, Mao M, Su D, Sun LL, Li KX, Qu Y, Sun Q, Zhang XY, Huang SY, et al.
Cell communication and signaling : CCS (2018) 161: 91. 116 002 WB; tested species: mouse
SNAP-25a and SNAP-25b differently mediate interactions with Munc18-1 and Gβγ subunits.
Daraio T, Valladolid-Acebes I, Brismar K, Bark C
Neuroscience letters (2018) 674: 75-80. 116 002 WB; tested species: mouse
MiR-335 overexpression impairs insulin secretion through defective priming of insulin vesicles.
Salunkhe VA, Ofori JK, Gandasi NR, Salö SA, Hansson S, Andersson ME, Wendt A, Barg S, Esguerra JLS, Eliasson L
Physiological reports (2017) 521: . 116 002 WB; tested species: rat
Potentiation of excitatory synaptic transmission ameliorates aggression in mice with Stxbp1 haploinsufficiency.
Miyamoto H, Shimohata A, Abe M, Abe T, Mazaki E, Amano K, Suzuki T, Tatsukawa T, Itohara S, Sakimura K, Yamakawa K, et al.
Human molecular genetics (2017) 2624: 4961-4974. 116 002 WB; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 116 002 WB
Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission.
Spangler SA, Schmitz SK, Kevenaar JT, de Graaff E, de Wit H, Demmers J, Toonen RF, Hoogenraad CC
The Journal of cell biology (2013) 2016: 915-28. 116 002 WB, ICC
mSYD1A, a mammalian synapse-defective-1 protein, regulates synaptogenic signaling and vesicle docking.
Wentzel C, Sommer JE, Nair R, Stiefvater A, Sibarita JB, Scheiffele P
Neuron (2013) 786: 1012-23. 116 002 WB; tested species: mouse
Role for Reelin in neurotransmitter release.
Hellwig S, Hack I, Kowalski J, Brunne B, Jarowyj J, Unger A, Bock HH, Junghans D, Frotscher M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 317: 2352-60. 116 002 WB
Abrogating Munc18-1-SNARE complex interaction has limited impact on exocytosis in PC12 cells.
Malintan NT, Nguyen TH, Han L, Latham CF, Osborne SL, Wen PJ, Lim SJ, Sugita S, Collins BM, Meunier FA
The Journal of biological chemistry (2009) 28432: 21637-46. 116 002 WB, ICC; tested species: rat
Preferential increase in the hippocampal synaptic vesicle protein 2A (SV2A) by pentylenetetrazole kindling.
Ohno Y, Ishihara S, Terada R, Kikuta M, Sofue N, Kawai Y, Serikawa T, Sasa M
Biochemical and biophysical research communications (2009) 3903: 415-20. 116 002 WB
Synaptic vesicle docking: sphingosine regulates syntaxin1 interaction with Munc18.
Camoletto PG, Vara H, Morando L, Connell E, Marletto FP, Giustetto M, Sassoè-Pognetto M, Van Veldhoven PP, Ledesma MD
PloS one (2009) 44: e5310. 116 002 WB
Proteomics of photoreceptor outer segments identifies a subset of SNARE and Rab proteins implicated in membrane vesicle trafficking and fusion.
Kwok MC, Holopainen JM, Molday LL, Foster LJ, Molday RS
Molecular & cellular proteomics : MCP (2008) 76: 1053-66. 116 002 WB
Neurotransmitter release regulated by a MALS-liprin-alpha presynaptic complex.
Olsen O, Moore KA, Fukata M, Kazuta T, Trinidad JC, Kauer FW, Streuli M, Misawa H, Burlingame AL, Nicoll RA, Bredt DS, et al.
The Journal of cell biology (2005) 1707: 1127-34. 116 002 WB
Granuphilin molecularly docks insulin granules to the fusion machinery.
Gomi H, Mizutani S, Kasai K, Itohara S, Izumi T
The Journal of cell biology (2005) 1711: 99-109. 116 002 WB
Munc18-syntaxin complexes and exocytosis in human platelets.
Houng A, Polgar J, Reed GL
The Journal of biological chemistry (2003) 27822: 19627-33. 116 002 WB
Rab27a mediates the tight docking of insulin granules onto the plasma membrane during glucose stimulation.
Kasai K, Ohara-Imaizumi M, Takahashi N, Mizutani S, Zhao S, Kikuta T, Kasai H, Nagamatsu S, Gomi H, Izumi T
The Journal of clinical investigation (2005) 1152: 388-96. 116 002 IP
Munc-18 associates with syntaxin and serves as a negative regulator of exocytosis in the pancreatic beta -cell.
Zhang W, Efanov A, Yang SN, Fried G, Kolare S, Brown H, Zaitsev S, Berggren PO, Meister B
The Journal of biological chemistry (2000) 27552: 41521-7. 116 002 IP, IHC
A density-based enrichment measure for assessing colocalization in single-molecule localization microscopy data.
Ejdrup AL, Lycas MD, Lorenzen N, Konomi A, Herborg F, Madsen KL, Gether U
Nature communications (2022) 131: 4388. 116 002 ICC; tested species: rat
Endophilin-A coordinates priming and fusion of neurosecretory vesicles via intersectin.
Gowrisankaran S, Houy S, Del Castillo JGP, Steubler V, Gelker M, Kroll J, Pinheiro PS, Schwitters D, Halbsgut N, Pechstein A, van Weering JRT, et al.
Nature communications (2020) 111: 1266. 116 002 WB, ICC; tested species: mouse
Autism and Schizophrenia-Associated CYFIP1 Regulates the Balance of Synaptic Excitation and Inhibition.
Davenport EC, Szulc BR, Drew J, Taylor J, Morgan T, Higgs NF, López-Doménech G, Kittler JT
Cell reports (2019) 268: 2037-2051.e6. 116 002 WB, ICC; tested species: mouse
Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission.
Spangler SA, Schmitz SK, Kevenaar JT, de Graaff E, de Wit H, Demmers J, Toonen RF, Hoogenraad CC
The Journal of cell biology (2013) 2016: 915-28. 116 002 WB, ICC
Munc18-1 controls SNARE protein complex assembly during human sperm acrosomal exocytosis.
Rodríguez F, Zanetti MN, Mayorga LS, Tomes CN
The Journal of biological chemistry (2012) 28752: 43825-39. 116 002 ICC; tested species: human
Munc18-1 tuning of vesicle merger and fusion pore properties.
Jorgacevski J, Potokar M, Grilc S, Kreft M, Liu W, Barclay JW, Bückers J, Medda R, Hell SW, Parpura V, Burgoyne RD, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3124: 9055-66. 116 002 ICC
Abrogating Munc18-1-SNARE complex interaction has limited impact on exocytosis in PC12 cells.
Malintan NT, Nguyen TH, Han L, Latham CF, Osborne SL, Wen PJ, Lim SJ, Sugita S, Collins BM, Meunier FA
The Journal of biological chemistry (2009) 28432: 21637-46. 116 002 WB, ICC; tested species: rat
Fusion-related release of glutamate from astrocytes.
Zhang Q, Pangrsic T, Kreft M, Krzan M, Li N, Sul JY, Halassa M, Van Bockstaele E, Zorec R, Haydon PG
The Journal of biological chemistry (2004) 27913: 12724-33. 116 002 ICC
Lifelong absence of microglia alters hippocampal glutamatergic networks but not synapse and spine density.
Surala M, Soso-Zdravkovic L, Munro D, Rifat A, Ouk K, Vida I, Priller J, Madry C
EMBO reports (2024) : . 116 002 IHC; tested species: mouse
Proteomic analysis reveals the composition of glutamatergic organelles of auditory inner hair cell.
Cepeda AP, Ninov M, Neef J, Parfentev I, Kusch K, Reisinger E, Jahn R, Moser T, Urlaub H
Molecular & cellular proteomics : MCP (2023) : 100704. 116 002 IHC; tested species: mouse
MAFA and MAFB regulate exocytosis-related genes in human β-cells.
Cataldo LR, Singh T, Achanta K, Bsharat S, Prasad RB, Luan C, Renström E, Eliasson L, Artner I
Acta physiologica (Oxford, England) (2022) 2342: e13761. 116 002 WB, IHC; tested species: mouse
Dysregulation of the SNARE-binding protein Munc18-1 impairs BDNF secretion and synaptic neurotransmission: a novel interventional target to protect the aging brain.
Lee YI, Kim YG, Pyeon HJ, Ahn JC, Logan S, Orock A, Joo KM, Lőrincz A, Deák F
GeroScience (2019) : . 116 002 WB, IHC; KD verified; tested species: mouse
Munc18-1 redistributes in nerve terminals in an activity- and PKC-dependent manner.
Cijsouw T, Weber JP, Broeke JH, Broek JA, Schut D, Kroon T, Saarloos I, Verhage M, Toonen RF
The Journal of cell biology (2014) 2045: 759-75. 116 002 IHC
Munc-18 associates with syntaxin and serves as a negative regulator of exocytosis in the pancreatic beta -cell.
Zhang W, Efanov A, Yang SN, Fried G, Kolare S, Brown H, Zaitsev S, Berggren PO, Meister B
The Journal of biological chemistry (2000) 27552: 41521-7. 116 002 IP, IHC
Membrane Location of Syntaxin-Binding Protein 1 Is Correlated with Poor Prognosis of Lung Adenocarcinoma.
Wang X, Fu G, Wen J, Chen H, Zhang B, Zhu D
The Tohoku journal of experimental medicine (2020) 2504: 263-270. 116 002 IHC-P; tested species: human
Total arrest of spontaneous and evoked synaptic transmission but normal synaptogenesis in the absence of Munc13-mediated vesicle priming.
Varoqueaux F, Sigler A, Rhee JS, Brose N, Enk C, Reim K, Rosenmund C
Proceedings of the National Academy of Sciences of the United States of America (2002) 9913: 9037-42. 116 002
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ICC
IHC
IHC-P
Background

Munc 18 is an abundant neuronal protein that tightly binds to the synaptic fusion protein syntaxin 1. It is highly homologous to the C. elegans unc-18 gene product, and weakly related to the yeast sec1, sly1, and slp1 genes.
There are three munc 18 isoforms in mammals. Munc 18-1 or 18a, also referred to as rb-sec1, n-sec1, stxbp1 and p67, is primarily expressed in neurons. Munc 18-2 or 18b, also referred to as stxbp2, and Munc 18-3 or 18c are expressed ubiquitously.
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