Synaptic Systems - Munc18-1

Cat. No.: 116 011

Amount: 100 µg

Price: $415.00

Cat. No. 116 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 1000 gallery IHC: not recommended IHC-P: not recommended ELISA: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Clone	131.1
Subtype	IgG2a (κ light chain)
Immunogen	Full-length rat Munc18-1 recombinant protein (UniProt Id: P61765)
Reactivity	Reacts with: rat (P61765), mouse (O08599). Other species not tested yet.
Specificity	Specific for Munc 18-1 with a minor cross-reactivity to Munc 18-2.
Remarks	WB: This antibody detects two smaller bands (possible degradation products) of unkown identity. ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 116 002 as detector antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet	116_011.pdf

References for Munc18-1 - 116 011

Enhanced hippocampal LTP but normal NMDA receptor and AMPA receptor function in a rat model of CDKL5 deficiency disorder.
Simões de Oliveira L, O'Leary HE, Nawaz S, Loureiro R, Davenport EC, Baxter P, Louros SR, Dando O, Perkins E, Peltier J, Trost M, et al.
Molecular autism (2024) 151: 28. 116 011 WB; tested species: rat

Light-dependent regulation of neurotransmitter release from rod photoreceptor ribbon synapses involves an interplay of Complexin 4 and Transducin with the SNARE complex.
Lux UT, Meyer J, Jahn O, Davison A, Babai N, Gießl A, Wartenberg A, Sticht H, Brose N, Reim K, Brandstätter JH, et al.
Frontiers in molecular neuroscience (2024) 17: 1308466. 116 011 WB; tested species: mouse

Age-dependent neurological phenotypes in a mouse model of PRRT2-related diseases.
Aj F, T M, C I, C BM, Kj N, H L, A N, Sm V, Y-H F, Lj P
Neurogenetics (2021) : . 116 011 WB; tested species: mouse

Maternal stress programs accelerated aging of the basal ganglia motor system in offspring.
Marrocco J, Verhaeghe R, Bucci D, Di Menna L, Traficante A, Bouwalerh H, Van Camp G, Ghiglieri V, Picconi B, Calabresi P, Ravasi L, et al.
Neurobiology of stress (2020) 13: 100265. 116 011 WB; tested species: rat

The reduction in glutamate release is predictive of cognitive and emotional alterations that are corrected by the positive modulator of AMPA receptors S 47445 in perinatal stressed rats.
Morley-Fletcher S, Zuena AR, Mairesse J, Gatta E, Van Camp G, Bouwalerh H, Riozzi B, Battaglia G, Pittaluga A, Olivero G, Mocaer E, et al.
Neuropharmacology (2018) 135: 284-296. 116 011 WB; tested species: rat

Potentiation of excitatory synaptic transmission ameliorates aggression in mice with Stxbp1 haploinsufficiency.
Miyamoto H, Shimohata A, Abe M, Abe T, Mazaki E, Amano K, Suzuki T, Tatsukawa T, Itohara S, Sakimura K, Yamakawa K, et al.
Human molecular genetics (2017) 2624: 4961-4974. 116 011 WB; tested species: mouse

Synaptic vesicle glycoprotein 2A (SV2A) regulates kindling epileptogenesis via GABAergic neurotransmission.
Tokudome K, Okumura T, Shimizu S, Mashimo T, Takizawa A, Serikawa T, Terada R, Ishihara S, Kunisawa N, Sasa M, Ohno Y, et al.
Scientific reports (2016) 6: 27420. 116 011 WB

The effects of antidepressant treatment in prenatally stressed rats support the glutamatergic hypothesis of stress-related disorders.
Marrocco J, Reynaert ML, Gatta E, Gabriel C, Mocaër E, Di Prisco S, Merega E, Pittaluga A, Nicoletti F, Maccari S, Morley-Fletcher S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 346: 2015-24. 116 011 WB; tested species: rat

Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 116 011 WB; tested species: mouse

Anxiety-like behavior of prenatally stressed rats is associated with a selective reduction of glutamate release in the ventral hippocampus.
Marrocco J, Mairesse J, Ngomba RT, Silletti V, Van Camp G, Bouwalerh H, Summa M, Pittaluga A, Nicoletti F, Maccari S, Morley-Fletcher S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3248: 17143-54. 116 011 WB

Munc18-1 regulates first-phase insulin release by promoting granule docking to multiple syntaxin isoforms.
Oh E, Kalwat MA, Kim MJ, Verhage M, Thurmond DC
The Journal of biological chemistry (2012) 28731: 25821-33. 116 011 WB; KO verified; tested species: mouse

Stimulus-induced S-nitrosylation of Syntaxin 4 impacts insulin granule exocytosis.
Wiseman DA, Kalwat MA, Thurmond DC
The Journal of biological chemistry (2011) 28618: 16344-54. 116 011 WB

Single synapse glutamate imaging reveals multiple levels of release mode regulation in mammalian synapses.
Farsi Z, Walde M, Klementowicz AE, Paraskevopoulou F, Woehler A
iScience (2021) 241: 101909. 116 011 ICC; tested species: rat

Riluzole attenuates the efficacy of glutamatergic transmission by interfering with the size of the readily releasable neurotransmitter pool.
Lazarevic V, Yang Y, Ivanova D, Fejtova A, Svenningsson P
Neuropharmacology (2018) : . 116 011 ICC; tested species: rat

Dendritic position is a major determinant of presynaptic strength.
de Jong AP, Schmitz SK, Toonen RF, Verhage M
The Journal of cell biology (2012) 1972: 327-37. 116 011 ICC

Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 116 011 ICC

Super-resolution imaging reveals the nanoscale organization of metabotropic glutamate receptors at presynaptic active zones.
Siddig S, Aufmkolk S, Doose S, Jobin ML, Werner C, Sauer M, Calebiro D
Science advances (2020) 616: eaay7193. 116 011 IHC; tested species: mouse

Cat. No.: 116 011

Amount: 100 µg

Price: $415.00

Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 116 011 WB; tested species: mouse

Stimulus-induced S-nitrosylation of Syntaxin 4 impacts insulin granule exocytosis.
Wiseman DA, Kalwat MA, Thurmond DC
The Journal of biological chemistry (2011) 28618: 16344-54. 116 011 WB

Dendritic position is a major determinant of presynaptic strength.
de Jong AP, Schmitz SK, Toonen RF, Verhage M
The Journal of cell biology (2012) 1972: 327-37. 116 011 ICC

ICC

Background

Munc 18 is an abundant neuronal protein that tightly binds to the synaptic fusion protein syntaxin 1. It is highly homologous to the C. elegans unc-18 gene product, and weakly related to the yeast sec1, sly1, and slp1 genes.
There are three munc 18 isoforms in mammals. Munc 18-1 or 18a, also referred to as rb-sec1, n-sec1, stxbp1 and p67, is primarily expressed in neurons. Munc 18-2 or 18b, also referred to as stxbp2, and Munc 18-3 or 18c are expressed ubiquitously.

1 ) Tellam JT et al., J. Biol. Chem. (1995)
2 ) Hata Y et al., J. Biol. Chem. (1995)
3 ) Pevsner J et al., Proc. Natl. Acad. Sci. U.S.A. (1994)
4 ) Garcia EP et al., Proc. Natl. Acad. Sci. U.S.A. (1994)
5 ) Hata Y et al., Nature (1993)
6 ) Fukuda M et al., J. Biol. Chem. (2005)
7 ) Martin-Verdeaux S et al., J. Cell. Sci. (2003)
8 ) Riento K et al., J. Biol. Chem. (2000)

Protocols

Standard protocol

ICC

ELISA

Membrane proteins

Other products for this target

Munc18-1 antibody - 116 011

References for Munc18-1 - 116 011