Tailor-made Antibodies
and Tools for Life Science

Munc18-1 antibody - 116 011

Munc18 proteins are peripheral membrane protein involved in vesicle docking and fusion
Mouse monoclonal purified IgG
Cat. No.: 116 011
Amount: 100 µg
Price: $420.00
Cat. No. 116 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 1000 gallery  
IHC: not recommended
IHC-P: not recommended
ELISA: yes (see remarks)
Clone 131.1
Subtype IgG2a (κ light chain)
Immunogen Full-length rat Munc18-1 recombinant protein (UniProt Id: P61765)
Reactivity Reacts with: rat (P61765), mouse (O08599).
Other species not tested yet.
Specificity Specific for Munc 18-1 with a minor cross-reactivity to Munc 18-2.
Remarks

WB: This antibody detects two smaller bands (possible degradation products) of unkown identity.
ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 116 002 as detector antibody. The ELISA-protocol for membrane proteins is recommended.

Data sheet 116_011.pdf

References for Munc18-1 - 116 011

Enhanced hippocampal LTP but normal NMDA receptor and AMPA receptor function in a rat model of CDKL5 deficiency disorder.
Simões de Oliveira L, O'Leary HE, Nawaz S, Loureiro R, Davenport EC, Baxter P, Louros SR, Dando O, Perkins E, Peltier J, Trost M, et al.
Molecular autism (2024) 151: 28. 116 011 WB; tested species: rat
Light-dependent regulation of neurotransmitter release from rod photoreceptor ribbon synapses involves an interplay of Complexin 4 and Transducin with the SNARE complex.
Lux UT, Meyer J, Jahn O, Davison A, Babai N, Gießl A, Wartenberg A, Sticht H, Brose N, Reim K, Brandstätter JH, et al.
Frontiers in molecular neuroscience (2024) 17: 1308466. 116 011 WB; tested species: mouse
Age-dependent neurological phenotypes in a mouse model of PRRT2-related diseases.
Aj F, T M, C I, C BM, Kj N, H L, A N, Sm V, Y-H F, Lj P
Neurogenetics (2021) : . 116 011 WB; tested species: mouse
Maternal stress programs accelerated aging of the basal ganglia motor system in offspring.
Marrocco J, Verhaeghe R, Bucci D, Di Menna L, Traficante A, Bouwalerh H, Van Camp G, Ghiglieri V, Picconi B, Calabresi P, Ravasi L, et al.
Neurobiology of stress (2020) 13: 100265. 116 011 WB; tested species: rat
The reduction in glutamate release is predictive of cognitive and emotional alterations that are corrected by the positive modulator of AMPA receptors S 47445 in perinatal stressed rats.
Morley-Fletcher S, Zuena AR, Mairesse J, Gatta E, Van Camp G, Bouwalerh H, Riozzi B, Battaglia G, Pittaluga A, Olivero G, Mocaer E, et al.
Neuropharmacology (2018) 135: 284-296. 116 011 WB; tested species: rat
Potentiation of excitatory synaptic transmission ameliorates aggression in mice with Stxbp1 haploinsufficiency.
Miyamoto H, Shimohata A, Abe M, Abe T, Mazaki E, Amano K, Suzuki T, Tatsukawa T, Itohara S, Sakimura K, Yamakawa K, et al.
Human molecular genetics (2017) 2624: 4961-4974. 116 011 WB; tested species: mouse
Synaptic vesicle glycoprotein 2A (SV2A) regulates kindling epileptogenesis via GABAergic neurotransmission.
Tokudome K, Okumura T, Shimizu S, Mashimo T, Takizawa A, Serikawa T, Terada R, Ishihara S, Kunisawa N, Sasa M, Ohno Y, et al.
Scientific reports (2016) 6: 27420. 116 011 WB
The effects of antidepressant treatment in prenatally stressed rats support the glutamatergic hypothesis of stress-related disorders.
Marrocco J, Reynaert ML, Gatta E, Gabriel C, Mocaër E, Di Prisco S, Merega E, Pittaluga A, Nicoletti F, Maccari S, Morley-Fletcher S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 346: 2015-24. 116 011 WB; tested species: rat
Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 116 011 WB; tested species: mouse
Anxiety-like behavior of prenatally stressed rats is associated with a selective reduction of glutamate release in the ventral hippocampus.
Marrocco J, Mairesse J, Ngomba RT, Silletti V, Van Camp G, Bouwalerh H, Summa M, Pittaluga A, Nicoletti F, Maccari S, Morley-Fletcher S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3248: 17143-54. 116 011 WB
Munc18-1 regulates first-phase insulin release by promoting granule docking to multiple syntaxin isoforms.
Oh E, Kalwat MA, Kim MJ, Verhage M, Thurmond DC
The Journal of biological chemistry (2012) 28731: 25821-33. 116 011 WB; KO verified; tested species: mouse
Stimulus-induced S-nitrosylation of Syntaxin 4 impacts insulin granule exocytosis.
Wiseman DA, Kalwat MA, Thurmond DC
The Journal of biological chemistry (2011) 28618: 16344-54. 116 011 WB
Cat. No.: 116 011
Amount: 100 µg
Price: $420.00