Synaptic Systems - Munc13-1

Cat. No.: 126 111

Amount: 100 µg

Price: $415.00

Cat. No. 126 111	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 5000 (AP staining) gallery IP: yes (see remarks) ICC: not tested yet IHC: not tested yet IHC-P: not tested yet ELISA: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Clone	266B1
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to AA 3 to 317 from rat Munc13-1 (UniProt Id: Q62768)
Reactivity	Reacts with: rat (Q62768), mouse (Q4KUS2), zebrafish. Other species not tested yet.
Specificity	K.O. validated
Remarks	IP: For most effective IP, use the denaturing IP-protocol. Consider that protein-protein interactions may be affected. ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 126 103 as detector antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet	126_111.pdf

References for Munc13-1 - 126 111

A presynaptic phosphosignaling hub for lasting homeostatic plasticity.
Müller JA, Betzin J, Santos-Tejedor J, Mayer A, Oprişoreanu AM, Engholm-Keller K, Paulußen I, Gulakova P, McGovern TD, Gschossman LJ, Schönhense E, et al.
Cell reports (2022) 393: 110696. 126 111 WB; tested species: mouse

CYP46A1 Activation by Efavirenz Leads to Behavioral Improvement without Significant Changes in Amyloid Plaque Load in the Brain of 5XFAD Mice.
Petrov AM, Lam M, Mast N, Moon J, Li Y, Maxfield E, Pikuleva IA
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics (2019) : . 126 111 WB; tested species: mouse

RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses.
Grauel MK, Maglione M, Reddy-Alla S, Willmes CG, Brockmann MM, Trimbuch T, Rosenmund T, Pangalos M, Vardar G, Stumpf A, Walter AM, et al.
Proceedings of the National Academy of Sciences of the United States of America (2016) 11341: 11615-11620. 126 111 WB; tested species: mouse

Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP.
Kaeser-Woo YJ, Younts TJ, Yang X, Zhou P, Wu D, Castillo PE, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3323: 9769-80. 126 111 WB

Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity.
Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, et al.
The Journal of biological chemistry (2013) 28848: 34906-19. 126 111 WB; tested species: mouse

Formation of Golgi-derived active zone precursor vesicles.
Maas C, Torres VI, Altrock WD, Leal-Ortiz S, Wagh D, Terry-Lorenzo RT, Fejtova A, Gundelfinger ED, Ziv NE, Garner CC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3232: 11095-108. 126 111 WB, ICC

Munc18-1 regulates first-phase insulin release by promoting granule docking to multiple syntaxin isoforms.
Oh E, Kalwat MA, Kim MJ, Verhage M, Thurmond DC
The Journal of biological chemistry (2012) 28731: 25821-33. 126 111 WB; tested species: mouse

Doc2b is a high-affinity Ca2+ sensor for spontaneous neurotransmitter release.
Groffen AJ, Martens S, Díez Arazola R, Cornelisse LN, Lozovaya N, de Jong AP, Goriounova NA, Habets RL, Takai Y, Borst JG, Brose N, et al.
Science (New York, N.Y.) (2010) 3275973: 1614-8. 126 111 WB; tested species: mouse

Munc13-4 is a limiting factor in the pathway required for platelet granule release and hemostasis.
Ren Q, Wimmer C, Chicka MC, Ye S, Ren Y, Hughson FM, Whiteheart SW
Blood (2010) 1166: 869-77. 126 111 WB; tested species: mouse

Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 126 111 WB

Active zone protein CAST is a component of conventional and ribbon synapses in mouse retina.
Deguchi-Tawarada M, Inoue E, Takao-Rikitsu E, Inoue M, Kitajima I, Ohtsuka T, Takai Y
The Journal of comparative neurology (2006) 4954: 480-96. 126 111 WB; tested species: mouse

Cast: a novel protein of the cytomatrix at the active zone of synapses that forms a ternary complex with RIM1 and munc13-1.
Ohtsuka T, Takao-Rikitsu E, Inoue E, Inoue M, Takeuchi M, Matsubara K, Deguchi-Tawarada M, Satoh K, Morimoto K, Nakanishi H, Takai Y, et al.
The Journal of cell biology (2002) 1583: 577-90. 126 111 WB; tested species: rat

Different Munc13 isoforms function as priming factors in lytic granule release from murine cytotoxic T lymphocytes.
Dudenhöffer-Pfeifer M, Schirra C, Pattu V, Halimani M, Maier-Peuschel M, Marshall MR, Matti U, Becherer U, Dirks J, Jung M, Lipp P, et al.
Traffic (Copenhagen, Denmark) (2013) 147: 798-809. 126 111 IP; tested species: mouse

The metabotropic glutamate receptor mGlu7 activates phospholipase C, translocates munc-13-1 protein, and potentiates glutamate release at cerebrocortical nerve terminals.
Martín R, Durroux T, Ciruela F, Torres M, Pin JP, Sánchez-Prieto J
The Journal of biological chemistry (2010) 28523: 17907-17. 126 111 IP

Vti1a/b regulate synaptic vesicle and dense core vesicle secretion via protein sorting at the Golgi.
Emperador-Melero J, Huson V, van Weering J, Bollmann C, Fischer von Mollard G, Toonen RF, Verhage M
Nature communications (2018) 91: 3421. 126 111 ICC; tested species: mouse

RIM C2B Domains Target Presynaptic Active Zone Functions to PIP2-Containing Membranes.
de Jong APH, Roggero CM, Ho MR, Wong MY, Brautigam CA, Rizo J, Kaeser PS
Neuron (2018) 982: 335-349.e7. 126 111 ICC; tested species: mouse

Studying synaptic efficiency by post-hoc immunolabelling.
Ramírez-Franco J, Alonso B, Bartolomé-Martín D, Sánchez-Prieto J, Torres M
BMC neuroscience (2013) 14: 127. 126 111 ICC

Efficient synaptic vesicle recycling after intense exocytosis concomitant with the accumulation of non-releasable endosomes at early developmental stages.
Bartolomé-Martín D, Ramírez-Franco J, Castro E, Sánchez-Prieto J, Torres M
Journal of cell science (2012) 125Pt 2: 422-34. 126 111 ICC; tested species: rat

Non-additive potentiation of glutamate release by phorbol esters and metabotropic mGlu7 receptor in cerebrocortical nerve terminals.
Martín R, Bartolomé-Martín D, Torres M, Sánchez-Prieto J
Journal of neurochemistry (2011) 1164: 476-85. 126 111 ICC; tested species: mouse

Cat. No.: 126 111

Amount: 100 µg

Price: $415.00

Studying synaptic efficiency by post-hoc immunolabelling.
Ramírez-Franco J, Alonso B, Bartolomé-Martín D, Sánchez-Prieto J, Torres M
BMC neuroscience (2013) 14: 127. 126 111 ICC

Background

Munc 13s are homologues of the C. elegans unc-13 gene product. Three brain specific isoforms, Munc 13-1, -13-2, and -13-3 are expressed in rat where they localize to presynaptic terminals. All three isoforms share multiple regulatory domains that may mediate phorbol ester and diacylglycerol binding.
Munc13-1 shows the broadest expression pattern and is found in cortex, cerebellum, olfactory bulb and hippocampus. Munc 13-2 is mainly expressed in cortex and hippocampus whereas Munc 13-3 exhibits highest expression levels in cerebellum and pons. Munc13-1 interacts directly with a putative coiled coil domain in the N-terminal part of syntaxin and is involved in synaptic vesicle priming. For Munc13-2 an additional ubiquitously expressed N-terminal splice variant (ubMunc 13-2) has been described.
Munc 13-3 has been shown to be involved in the regulation of cerebellar synaptic transmission and motor learning.

1 ) Sheu L et al., J. Biol. Chem. (2003)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Ohtsuka T et al., J. Cell Biol. (2002)
4 ) Ashery U et al., EMBO J. (2000)
5 ) Brose N et al., Curr. Opin. Neurobiol. (2000)
6 ) Betz A et al., J. Biol. Chem. (1997)
7 ) Südhof TC et al., Nature (1995)
8 ) Brose N et al., J. Biol. Chem. (1995)
9 ) Goldenberg NM et al., Am. J. Physiol., Cell Physiol. (2009)
10 ) Zhu Y et al., J. Physiol. (Lond.) (2008)

Protocols

ELISA

Membrane proteins

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Munc13-1 antibody - 126 111 K.O.

References for Munc13-1 - 126 111