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Home > Products > 126 103

Munc13-1 antibody - 126 103 K.O.

Munc13 proteins are the mammalian homologues of the C. elegans unc-13 gene essential for exocytosis
Rabbit polyclonal purified antibody
Cat. No.: 126 103
Amount: 50 µg
Price: $475.00
Cat. No. 126 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes (see remarks)
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 200 gallery  
IHC-P: not tested yet
ExM: yes
DNA-PAINT: yes
EM: yes gallery  
ELISA: yes (see remarks)
Immunogen Recombinant protein corresponding to AA 3 to 317 from rat Munc13-1 (UniProt Id: Q62768)
Reactivity Reacts with: human (Q9UPW8), rat (Q62768), mouse (Q4KUS2), zebrafish.
Other species not tested yet.
Specificity K.O. validated PubMed: 28772123
Remarks

IP: For most effective IP, use the denaturing IP-protocol. Consider that protein-protein interactions may be affected.
ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 126 111 as capture antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet 126_103.pdf

References for Munc13-1 - 126 103

Liprin-α proteins are master regulators of human presynapse assembly.
Marcó de la Cruz B, Campos J, Molinaro A, Xie X, Jin G, Wei Z, Acuna C, Sterky FH
Nature neuroscience (2024) : . 126 103 WB, ICC; tested species: human
Munc13 supports fusogenicity of non-docked vesicles at synapses with disrupted active zones.
Tan C, de Nola G, Qiao C, Imig C, Born RT, Brose N, Kaeser PS
eLife (2022) 11: . 126 103 WB, ICC; KO verified; tested species: mouse
Bassoon controls synaptic vesicle release via regulation of presynaptic phosphorylation and cAMP.
Montenegro-Venegas C, Guhathakurta D, Pina-Fernandez E, Andres-Alonso M, Plattner F, Gundelfinger ED, Fejtova A
EMBO reports (2022) 238: e53659. 126 103 WB, ICC; tested species: mouse
TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13A.
Brown AL, Wilkins OG, Keuss MJ, Hill SE, Zanovello M, Lee WC, Bampton A, Lee FCY, Masino L, Qi YA, Bryce-Smith S, et al.
Nature (2022) 6037899: 131-137. 126 103 WB; tested species: human
An active vesicle priming machinery suppresses axon regeneration upon adult CNS injury.
Hilton BJ, Husch A, Schaffran B, Lin TC, Burnside ER, Dupraz S, Schelski M, Kim J, Müller JA, Schoch S, Imig C, et al.
Neuron (2021) : . 126 103 WB, ICC; KO verified; tested species: mouse
Munc13-1 is a Ca2+-phospholipid-dependent vesicle priming hub that shapes synaptic short-term plasticity and enables sustained neurotransmission.
Lipstein N, Chang S, Lin KH, López-Murcia FJ, Neher E, Taschenberger H, Brose N
Neuron (2021) : . 126 103 WB, IHC; tested species: mouse
Actions of Rab27B-GTPase on mammalian central excitatory synaptic transmission.
Arias-Hervert ER, Xu N, Njus M, Murphy GG, Hou Y, Williams JA, Lentz SI, Ernst SA, Stuenkel EL
Physiological reports (2020) 89: e14428. 126 103 WB; tested species: mouse
Receptor protein tyrosine phosphatase delta is not essential for synapse maintenance or transmission at hippocampal synapses.
Han KA, Lee HY, Lim D, Shin J, Yoon TH, Liu X, Um JW, Choi SY, Ko J
Molecular brain (2020) 131: 94. 126 103 WB; tested species: mouse
Disentangling the roles of RIM and Munc13 in synaptic vesicle localization and neurotransmission.
Zarebidaki F, Camacho M, Brockmann MM, Trimbuch T, Herman MA, Rosenmund C
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 126 103 WB; KD verified; tested species: mouse
Autaptic cultures of human induced neurons as a versatile platform for studying synaptic function and neuronal morphology.
Fenske P, Grauel MK, Brockmann MM, Dorrn AL, Trimbuch T, Rosenmund C
Scientific reports (2019) 91: 4890. 126 103 WB; KD verified; tested species: human
Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts.
Raja MK, Preobraschenski J, Del Olmo-Cabrera S, Martinez-Turrillas R, Jahn R, Perez-Otano I, Wesseling JF
eLife (2019) 8: . 126 103 WB; tested species: mouse
Postsynaptic RIM1 modulates synaptic function by facilitating membrane delivery of recycling NMDARs in hippocampal neurons.
Wang J, Lv X, Wu Y, Xu T, Jiao M, Yang R, Li X, Chen M, Yan Y, Chen C, Dong W, et al.
Nature communications (2018) 91: 2267. 126 103 WB; tested species: mouse
Inhibitory role of Munc13-1 in antigen-induced mast cell degranulation.
Higashio H, Satoh YI, Saino T
Biomedical research (Tokyo, Japan) (2017) 386: 321-329. 126 103 WB; tested species: rat
Molecular Mechanisms of Synaptic Vesicle Priming by Munc13 and Munc18.
Lai Y, Choi UB, Leitz J, Rhee HJ, Lee C, Altas B, Zhao M, Pfuetzner RA, Wang AL, Brose N, Rhee J, et al.
Neuron (2017) 953: 591-607.e10. 126 103 WB; KO verified; tested species: mouse
How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 126 103 WB
Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 126 103 WB; tested species: rat
The Munc13 proteins differentially regulate readily releasable pool dynamics and calcium-dependent recovery at a central synapse.
Chen Z, Cooper B, Kalla S, Varoqueaux F, Young SM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3319: 8336-51. 126 103 WB
β-Adrenergic receptors activate exchange protein directly activated by cAMP (Epac), translocate Munc13-1, and enhance the Rab3A-RIM1α interaction to potentiate glutamate release at cerebrocortical nerve terminals.
Ferrero JJ, Alvarez AM, Ramírez-Franco J, Godino MC, Bartolomé-Martín D, Aguado C, Torres M, Luján R, Ciruela F, Sánchez-Prieto J
The Journal of biological chemistry (2013) 28843: 31370-85. 126 103 WB; tested species: mouse
Different Munc13 isoforms function as priming factors in lytic granule release from murine cytotoxic T lymphocytes.
Dudenhöffer-Pfeifer M, Schirra C, Pattu V, Halimani M, Maier-Peuschel M, Marshall MR, Matti U, Becherer U, Dirks J, Jung M, Lipp P, et al.
Traffic (Copenhagen, Denmark) (2013) 147: 798-809. 126 103 WB
RIM, Munc13, and Rab3A interplay in acrosomal exocytosis.
Bello OD, Zanetti MN, Mayorga LS, Michaut MA
Experimental cell research (2012) 3185: 478-88. 126 103 WB, ICC
Non-additive potentiation of glutamate release by phorbol esters and metabotropic mGlu7 receptor in cerebrocortical nerve terminals.
Martín R, Bartolomé-Martín D, Torres M, Sánchez-Prieto J
Journal of neurochemistry (2011) 1164: 476-85. 126 103 ICC, WB; tested species: mouse
Loss of postsynaptic NMDARs drives nanoscale reorganization of Munc13-1 and PSD-95.
Dharmasri PA, DeMarco EM, Anderson MC, Levy AD, Blanpied TA
bioRxiv : the preprint server for biology (2024) : . 126 103 ICC, DNA-PAINT; tested species: rat
Liprin-α proteins are master regulators of human presynapse assembly.
Marcó de la Cruz B, Campos J, Molinaro A, Xie X, Jin G, Wei Z, Acuna C, Sterky FH
Nature neuroscience (2024) : . 126 103 WB, ICC; tested species: human
Differential nanoscale organization of excitatory synapses onto excitatory vs inhibitory neurons.
Dharmasri PA, Levy AD, Blanpied TA
bioRxiv : the preprint server for biology (2023) : . 126 103 ICC; tested species: rat
Molecular definition of distinct active zone protein machineries for Ca2+ channel clustering and synaptic vesicle priming.
Emperador-Melero J, Andersen JW, Metzbower SR, Levy AD, Dharmasri PA, de Nola G, Blanpied TA, Kaeser PS
bioRxiv : the preprint server for biology (2023) : . 126 103 ICC, DNA-PAINT; tested species: mouse
Presynapses contain distinct actin nanostructures.
Bingham D, Jakobs CE, Wernert F, Boroni-Rueda F, Jullien N, Schentarra EM, Friedl K, Da Costa Moura J, van Bommel DM, Caillol G, Ogawa Y, et al.
The Journal of cell biology (2023) 22210: . 126 103 ICC; tested species: rat
Rebuilding essential active zone functions within a synapse.
Tan C, Wang SSH, de Nola G, Kaeser PS
Neuron (2022) 1109: 1498-1515.e8. 126 103 ICC; tested species: mouse
Munc13 supports fusogenicity of non-docked vesicles at synapses with disrupted active zones.
Tan C, de Nola G, Qiao C, Imig C, Born RT, Brose N, Kaeser PS
eLife (2022) 11: . 126 103 WB, ICC; KO verified; tested species: mouse
Bassoon controls synaptic vesicle release via regulation of presynaptic phosphorylation and cAMP.
Montenegro-Venegas C, Guhathakurta D, Pina-Fernandez E, Andres-Alonso M, Plattner F, Gundelfinger ED, Fejtova A
EMBO reports (2022) 238: e53659. 126 103 WB, ICC; tested species: mouse
Intact synapse structure and function after combined knockout of PTPδ, PTPσ, and LAR.
Emperador-Melero J, de Nola G, Kaeser PS
eLife (2021) 10: . 126 103 ICC; tested species: mouse
PKC-phosphorylation of Liprin-α3 triggers phase separation and controls presynaptic active zone structure.
Emperador-Melero J, Wong MY, Wang SSH, de Nola G, Nyitrai H, Kirchhausen T, Kaeser PS
Nature communications (2021) 121: 3057. 126 103 ICC; tested species: mouse
An active vesicle priming machinery suppresses axon regeneration upon adult CNS injury.
Hilton BJ, Husch A, Schaffran B, Lin TC, Burnside ER, Dupraz S, Schelski M, Kim J, Müller JA, Schoch S, Imig C, et al.
Neuron (2021) : . 126 103 WB, ICC; KO verified; tested species: mouse
Single synapse glutamate imaging reveals multiple levels of release mode regulation in mammalian synapses.
Farsi Z, Walde M, Klementowicz AE, Paraskevopoulou F, Woehler A
iScience (2021) 241: 101909. 126 103 ICC; tested species: rat
Bassoon inhibits proteasome activity via interaction with PSMB4.
Montenegro-Venegas C, Fienko S, Anni D, Pina-Fernández E, Frischknecht R, Fejtova A
Cellular and molecular life sciences : CMLS (2020) : . 126 103 ICC; tested species: mouse
Synapse and Active Zone Assembly in the Absence of Presynaptic Ca2+ Channels and Ca2+ Entry.
Held RG, Liu C, Ma K, Ramsey AM, Tarr TB, De Nola G, Wang SSH, Wang J, van den Maagdenberg AMJM, Schneider T, Sun J, et al.
Neuron (2020) 1074: 667-683.e9. 126 103 ICC; tested species: mouse
Synaptic neurexin-1 assembles into dynamically regulated active zone nanoclusters.
Trotter JH, Hao J, Maxeiner S, Tsetsenis T, Liu Z, Zhuang X, Südhof TC
The Journal of cell biology (2019) : . 126 103 ICC; tested species: mouse
Transient Confinement of CaV2.1 Ca2+-Channel Splice Variants Shapes Synaptic Short-Term Plasticity.
Heck J, Parutto P, Ciuraszkiewicz A, Bikbaev A, Freund R, Mitlöhner J, Alonso M, Fejtova A, Holcman D, Heine M
Neuron (2019) : . 126 103 ICC; tested species: rat
Visualization of Synchronous or Asynchronous Release of Single Synaptic Vesicle in Active-Zone-Like Membrane Formed on Neuroligin-Coated Glass Surface.
Funahashi J, Tanaka H, Hirano T
Frontiers in cellular neuroscience (2018) 12: 140. 126 103 ICC; tested species: rat
Riluzole attenuates the efficacy of glutamatergic transmission by interfering with the size of the readily releasable neurotransmitter pool.
Lazarevic V, Yang Y, Ivanova D, Fejtova A, Svenningsson P
Neuropharmacology (2018) : . 126 103 ICC; tested species: rat
Dopamine Secretion Is Mediated by Sparse Active Zone-like Release Sites.
Liu C, Kershberg L, Wang J, Schneeberger S, Kaeser PS
Cell (2018) 1724: 706-718.e15. 126 103 ICC; tested species: mouse
CAPS-1 promotes fusion competence of stationary dense-core vesicles in presynaptic terminals of mammalian neurons.
Farina M, van de Bospoort R, He E, Persoon CM, van Weering JR, Broeke JH, Verhage M, Toonen RF
eLife (2015) 4: . 126 103 ICC
mSYD1A, a mammalian synapse-defective-1 protein, regulates synaptogenic signaling and vesicle docking.
Wentzel C, Sommer JE, Nair R, Stiefvater A, Sibarita JB, Scheiffele P
Neuron (2013) 786: 1012-23. 126 103 ICC; tested species: mouse
RIM, Munc13, and Rab3A interplay in acrosomal exocytosis.
Bello OD, Zanetti MN, Mayorga LS, Michaut MA
Experimental cell research (2012) 3185: 478-88. 126 103 WB, ICC
Non-additive potentiation of glutamate release by phorbol esters and metabotropic mGlu7 receptor in cerebrocortical nerve terminals.
Martín R, Bartolomé-Martín D, Torres M, Sánchez-Prieto J
Journal of neurochemistry (2011) 1164: 476-85. 126 103 ICC, WB; tested species: mouse
A role for the ubiquitin-proteasome system in activity-dependent presynaptic silencing.
Jiang X, Litkowski PE, Taylor AA, Lin Y, Snider BJ, Moulder KL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 305: 1798-809. 126 103 ICC
Dynein light chain regulates axonal trafficking and synaptic levels of Bassoon.
Fejtova A, Davydova D, Bischof F, Lazarevic V, Altrock WD, Romorini S, Schöne C, Zuschratter W, Kreutz MR, Garner CC, Ziv NE, et al.
The Journal of cell biology (2009) 1852: 341-55. 126 103 ICC
Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis.
Leal-Ortiz S, Waites CL, Terry-Lorenzo R, Zamorano P, Gundelfinger ED, Garner CC
The Journal of cell biology (2008) 1815: 831-46. 126 103 ICC
Combinatorial expression of neurexins and LAR-type phosphotyrosine phosphatase receptors instructs assembly of a cerebellar circuit.
Sclip A, Südhof TC
Nature communications (2023) 141: 4976. 126 103 IHC; tested species: mouse
Laser microscopy acquisition and analysis of premotor synapses in the murine spinal cord.
Buettner JM, Kirmann T, Mentis GZ, Hallermann S, Simon CM
STAR protocols (2022) 31: 101236. 126 103 IHC; tested species: mouse
Munc13-1 is a Ca2+-phospholipid-dependent vesicle priming hub that shapes synaptic short-term plasticity and enables sustained neurotransmission.
Lipstein N, Chang S, Lin KH, López-Murcia FJ, Neher E, Taschenberger H, Brose N
Neuron (2021) : . 126 103 WB, IHC; tested species: mouse
Variability in the Munc13-1 content of excitatory release sites.
Karlocai MR, Heredi J, Benedek T, Holderith N, Lorincz A, Nusser Z
eLife (2021) 10: . 126 103 IHC, EM; tested species: mouse
Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue.
Vatan T, Minehart JA, Zhang C, Agarwal V, Yang J, Speer CM
STAR protocols (2021) 24: 100971. 126 103 IHC; tested species: mouse
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 126 103 IHC; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 126 103 IHC; tested species: mouse
Bassoon-disruption slows vesicle replenishment and induces homeostatic plasticity at a CNS synapse.
Mendoza Schulz A, Jing Z, Sánchez Caro JM, Wetzel F, Dresbach T, Strenzke N, Wichmann C, Moser T
The EMBO journal (2014) 335: 512-27. 126 103 IHC
Deficits in morphofunctional maturation of hippocampal mossy fiber synapses in a mouse model of intellectual disability.
Lanore F, Labrousse VF, Szabo Z, Normand E, Blanchet C, Mulle C
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3249: 17882-93. 126 103 IHC
Impaired development of hippocampal mossy fibre synapses in mouse mutants for the presynaptic scaffold protein Bassoon.
Lanore F, Blanchet C, Fejtova A, Pinheiro P, Richter K, Balschun D, Gundelfinger E, Mulle C
The Journal of physiology (2010) 588Pt 12: 2133-45. 126 103 IHC; tested species: mouse
Active zone proteins are dynamically associated with synaptic ribbons in rat pinealocytes.
Spiwoks-Becker I, Maus C, tom Dieck S, Fejtová A, Engel L, Wolloscheck T, Wolfrum U, Vollrath L, Spessert R
Cell and tissue research (2008) 3332: 185-95. 126 103 IHC
Enhanced synaptic protein visualization by multicolor super-resolution expansion microscopy.
Eilts J, Reinhard S, Michetschläger N, Werner C, Sauer M
Neurophotonics (2023) 104: 044412. 126 103 ExM; tested species: mouse
Loss of postsynaptic NMDARs drives nanoscale reorganization of Munc13-1 and PSD-95.
Dharmasri PA, DeMarco EM, Anderson MC, Levy AD, Blanpied TA
bioRxiv : the preprint server for biology (2024) : . 126 103 ICC, DNA-PAINT; tested species: rat
Molecular definition of distinct active zone protein machineries for Ca2+ channel clustering and synaptic vesicle priming.
Emperador-Melero J, Andersen JW, Metzbower SR, Levy AD, Dharmasri PA, de Nola G, Blanpied TA, Kaeser PS
bioRxiv : the preprint server for biology (2023) : . 126 103 ICC, DNA-PAINT; tested species: mouse
Gold In-and-Out: A Toolkit for Analyzing Subcellular Distribution of Immunogold-Labeled Membrane Proteins in Freeze-Fracture Replica Images.
Guerrero-Given D, Goldin SL, Thomas CI, Anthony SA, Jerez D, Kamasawa N
Frontiers in neuroanatomy (2022) 16: 855218. 126 103 EM; tested species: mouse
Different priming states of synaptic vesicles underlie distinct release probabilities at hippocampal excitatory synapses.
Aldahabi M, Balint F, Holderith N, Lorincz A, Reva M, Nusser Z
Neuron (2022) : . 126 103 EM; tested species: mouse
Variability in the Munc13-1 content of excitatory release sites.
Karlocai MR, Heredi J, Benedek T, Holderith N, Lorincz A, Nusser Z
eLife (2021) 10: . 126 103 IHC, EM; tested species: mouse
Distinct Nanoscale Calcium Channel and Synaptic Vesicle Topographies Contribute to the Diversity of Synaptic Function.
Rebola N, Reva M, Kirizs T, Szoboszlay M, Lőrincz A, Moneron G, Nusser Z, DiGregorio DA
Neuron (2019) 1044: 693-710.e9. 126 103 EM; tested species: mouse
Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.
Vasileva M, Horstmann H, Geumann C, Gitler D, Kuner T
The European journal of neuroscience (2012) 368: 3005-20. 126 103 ELISA
Cat. No.: 126 103
Amount: 50 µg
Price: $475.00
Liprin-α proteins are master regulators of human presynapse assembly.
Marcó de la Cruz B, Campos J, Molinaro A, Xie X, Jin G, Wei Z, Acuna C, Sterky FH
Nature neuroscience (2024) : . 126 103 WB, ICC; tested species: human
Munc13 supports fusogenicity of non-docked vesicles at synapses with disrupted active zones.
Tan C, de Nola G, Qiao C, Imig C, Born RT, Brose N, Kaeser PS
eLife (2022) 11: . 126 103 WB, ICC; KO verified; tested species: mouse
Bassoon controls synaptic vesicle release via regulation of presynaptic phosphorylation and cAMP.
Montenegro-Venegas C, Guhathakurta D, Pina-Fernandez E, Andres-Alonso M, Plattner F, Gundelfinger ED, Fejtova A
EMBO reports (2022) 238: e53659. 126 103 WB, ICC; tested species: mouse
TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13A.
Brown AL, Wilkins OG, Keuss MJ, Hill SE, Zanovello M, Lee WC, Bampton A, Lee FCY, Masino L, Qi YA, Bryce-Smith S, et al.
Nature (2022) 6037899: 131-137. 126 103 WB; tested species: human
An active vesicle priming machinery suppresses axon regeneration upon adult CNS injury.
Hilton BJ, Husch A, Schaffran B, Lin TC, Burnside ER, Dupraz S, Schelski M, Kim J, Müller JA, Schoch S, Imig C, et al.
Neuron (2021) : . 126 103 WB, ICC; KO verified; tested species: mouse
Munc13-1 is a Ca2+-phospholipid-dependent vesicle priming hub that shapes synaptic short-term plasticity and enables sustained neurotransmission.
Lipstein N, Chang S, Lin KH, López-Murcia FJ, Neher E, Taschenberger H, Brose N
Neuron (2021) : . 126 103 WB, IHC; tested species: mouse
Actions of Rab27B-GTPase on mammalian central excitatory synaptic transmission.
Arias-Hervert ER, Xu N, Njus M, Murphy GG, Hou Y, Williams JA, Lentz SI, Ernst SA, Stuenkel EL
Physiological reports (2020) 89: e14428. 126 103 WB; tested species: mouse
Receptor protein tyrosine phosphatase delta is not essential for synapse maintenance or transmission at hippocampal synapses.
Han KA, Lee HY, Lim D, Shin J, Yoon TH, Liu X, Um JW, Choi SY, Ko J
Molecular brain (2020) 131: 94. 126 103 WB; tested species: mouse
Disentangling the roles of RIM and Munc13 in synaptic vesicle localization and neurotransmission.
Zarebidaki F, Camacho M, Brockmann MM, Trimbuch T, Herman MA, Rosenmund C
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 126 103 WB; KD verified; tested species: mouse
Autaptic cultures of human induced neurons as a versatile platform for studying synaptic function and neuronal morphology.
Fenske P, Grauel MK, Brockmann MM, Dorrn AL, Trimbuch T, Rosenmund C
Scientific reports (2019) 91: 4890. 126 103 WB; KD verified; tested species: human
Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts.
Raja MK, Preobraschenski J, Del Olmo-Cabrera S, Martinez-Turrillas R, Jahn R, Perez-Otano I, Wesseling JF
eLife (2019) 8: . 126 103 WB; tested species: mouse
Postsynaptic RIM1 modulates synaptic function by facilitating membrane delivery of recycling NMDARs in hippocampal neurons.
Wang J, Lv X, Wu Y, Xu T, Jiao M, Yang R, Li X, Chen M, Yan Y, Chen C, Dong W, et al.
Nature communications (2018) 91: 2267. 126 103 WB; tested species: mouse
Inhibitory role of Munc13-1 in antigen-induced mast cell degranulation.
Higashio H, Satoh YI, Saino T
Biomedical research (Tokyo, Japan) (2017) 386: 321-329. 126 103 WB; tested species: rat
Molecular Mechanisms of Synaptic Vesicle Priming by Munc13 and Munc18.
Lai Y, Choi UB, Leitz J, Rhee HJ, Lee C, Altas B, Zhao M, Pfuetzner RA, Wang AL, Brose N, Rhee J, et al.
Neuron (2017) 953: 591-607.e10. 126 103 WB; KO verified; tested species: mouse
How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 126 103 WB
Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 126 103 WB; tested species: rat
The Munc13 proteins differentially regulate readily releasable pool dynamics and calcium-dependent recovery at a central synapse.
Chen Z, Cooper B, Kalla S, Varoqueaux F, Young SM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3319: 8336-51. 126 103 WB
β-Adrenergic receptors activate exchange protein directly activated by cAMP (Epac), translocate Munc13-1, and enhance the Rab3A-RIM1α interaction to potentiate glutamate release at cerebrocortical nerve terminals.
Ferrero JJ, Alvarez AM, Ramírez-Franco J, Godino MC, Bartolomé-Martín D, Aguado C, Torres M, Luján R, Ciruela F, Sánchez-Prieto J
The Journal of biological chemistry (2013) 28843: 31370-85. 126 103 WB; tested species: mouse
Different Munc13 isoforms function as priming factors in lytic granule release from murine cytotoxic T lymphocytes.
Dudenhöffer-Pfeifer M, Schirra C, Pattu V, Halimani M, Maier-Peuschel M, Marshall MR, Matti U, Becherer U, Dirks J, Jung M, Lipp P, et al.
Traffic (Copenhagen, Denmark) (2013) 147: 798-809. 126 103 WB
RIM, Munc13, and Rab3A interplay in acrosomal exocytosis.
Bello OD, Zanetti MN, Mayorga LS, Michaut MA
Experimental cell research (2012) 3185: 478-88. 126 103 WB, ICC
Non-additive potentiation of glutamate release by phorbol esters and metabotropic mGlu7 receptor in cerebrocortical nerve terminals.
Martín R, Bartolomé-Martín D, Torres M, Sánchez-Prieto J
Journal of neurochemistry (2011) 1164: 476-85. 126 103 ICC, WB; tested species: mouse
Loss of postsynaptic NMDARs drives nanoscale reorganization of Munc13-1 and PSD-95.
Dharmasri PA, DeMarco EM, Anderson MC, Levy AD, Blanpied TA
bioRxiv : the preprint server for biology (2024) : . 126 103 ICC, DNA-PAINT; tested species: rat
Liprin-α proteins are master regulators of human presynapse assembly.
Marcó de la Cruz B, Campos J, Molinaro A, Xie X, Jin G, Wei Z, Acuna C, Sterky FH
Nature neuroscience (2024) : . 126 103 WB, ICC; tested species: human
Differential nanoscale organization of excitatory synapses onto excitatory vs inhibitory neurons.
Dharmasri PA, Levy AD, Blanpied TA
bioRxiv : the preprint server for biology (2023) : . 126 103 ICC; tested species: rat
Molecular definition of distinct active zone protein machineries for Ca2+ channel clustering and synaptic vesicle priming.
Emperador-Melero J, Andersen JW, Metzbower SR, Levy AD, Dharmasri PA, de Nola G, Blanpied TA, Kaeser PS
bioRxiv : the preprint server for biology (2023) : . 126 103 ICC, DNA-PAINT; tested species: mouse
Presynapses contain distinct actin nanostructures.
Bingham D, Jakobs CE, Wernert F, Boroni-Rueda F, Jullien N, Schentarra EM, Friedl K, Da Costa Moura J, van Bommel DM, Caillol G, Ogawa Y, et al.
The Journal of cell biology (2023) 22210: . 126 103 ICC; tested species: rat
Rebuilding essential active zone functions within a synapse.
Tan C, Wang SSH, de Nola G, Kaeser PS
Neuron (2022) 1109: 1498-1515.e8. 126 103 ICC; tested species: mouse
Munc13 supports fusogenicity of non-docked vesicles at synapses with disrupted active zones.
Tan C, de Nola G, Qiao C, Imig C, Born RT, Brose N, Kaeser PS
eLife (2022) 11: . 126 103 WB, ICC; KO verified; tested species: mouse
Bassoon controls synaptic vesicle release via regulation of presynaptic phosphorylation and cAMP.
Montenegro-Venegas C, Guhathakurta D, Pina-Fernandez E, Andres-Alonso M, Plattner F, Gundelfinger ED, Fejtova A
EMBO reports (2022) 238: e53659. 126 103 WB, ICC; tested species: mouse
Intact synapse structure and function after combined knockout of PTPδ, PTPσ, and LAR.
Emperador-Melero J, de Nola G, Kaeser PS
eLife (2021) 10: . 126 103 ICC; tested species: mouse
PKC-phosphorylation of Liprin-α3 triggers phase separation and controls presynaptic active zone structure.
Emperador-Melero J, Wong MY, Wang SSH, de Nola G, Nyitrai H, Kirchhausen T, Kaeser PS
Nature communications (2021) 121: 3057. 126 103 ICC; tested species: mouse
An active vesicle priming machinery suppresses axon regeneration upon adult CNS injury.
Hilton BJ, Husch A, Schaffran B, Lin TC, Burnside ER, Dupraz S, Schelski M, Kim J, Müller JA, Schoch S, Imig C, et al.
Neuron (2021) : . 126 103 WB, ICC; KO verified; tested species: mouse
Single synapse glutamate imaging reveals multiple levels of release mode regulation in mammalian synapses.
Farsi Z, Walde M, Klementowicz AE, Paraskevopoulou F, Woehler A
iScience (2021) 241: 101909. 126 103 ICC; tested species: rat
Bassoon inhibits proteasome activity via interaction with PSMB4.
Montenegro-Venegas C, Fienko S, Anni D, Pina-Fernández E, Frischknecht R, Fejtova A
Cellular and molecular life sciences : CMLS (2020) : . 126 103 ICC; tested species: mouse
Synapse and Active Zone Assembly in the Absence of Presynaptic Ca2+ Channels and Ca2+ Entry.
Held RG, Liu C, Ma K, Ramsey AM, Tarr TB, De Nola G, Wang SSH, Wang J, van den Maagdenberg AMJM, Schneider T, Sun J, et al.
Neuron (2020) 1074: 667-683.e9. 126 103 ICC; tested species: mouse
Synaptic neurexin-1 assembles into dynamically regulated active zone nanoclusters.
Trotter JH, Hao J, Maxeiner S, Tsetsenis T, Liu Z, Zhuang X, Südhof TC
The Journal of cell biology (2019) : . 126 103 ICC; tested species: mouse
Transient Confinement of CaV2.1 Ca2+-Channel Splice Variants Shapes Synaptic Short-Term Plasticity.
Heck J, Parutto P, Ciuraszkiewicz A, Bikbaev A, Freund R, Mitlöhner J, Alonso M, Fejtova A, Holcman D, Heine M
Neuron (2019) : . 126 103 ICC; tested species: rat
Visualization of Synchronous or Asynchronous Release of Single Synaptic Vesicle in Active-Zone-Like Membrane Formed on Neuroligin-Coated Glass Surface.
Funahashi J, Tanaka H, Hirano T
Frontiers in cellular neuroscience (2018) 12: 140. 126 103 ICC; tested species: rat
Riluzole attenuates the efficacy of glutamatergic transmission by interfering with the size of the readily releasable neurotransmitter pool.
Lazarevic V, Yang Y, Ivanova D, Fejtova A, Svenningsson P
Neuropharmacology (2018) : . 126 103 ICC; tested species: rat
Dopamine Secretion Is Mediated by Sparse Active Zone-like Release Sites.
Liu C, Kershberg L, Wang J, Schneeberger S, Kaeser PS
Cell (2018) 1724: 706-718.e15. 126 103 ICC; tested species: mouse
CAPS-1 promotes fusion competence of stationary dense-core vesicles in presynaptic terminals of mammalian neurons.
Farina M, van de Bospoort R, He E, Persoon CM, van Weering JR, Broeke JH, Verhage M, Toonen RF
eLife (2015) 4: . 126 103 ICC
mSYD1A, a mammalian synapse-defective-1 protein, regulates synaptogenic signaling and vesicle docking.
Wentzel C, Sommer JE, Nair R, Stiefvater A, Sibarita JB, Scheiffele P
Neuron (2013) 786: 1012-23. 126 103 ICC; tested species: mouse
RIM, Munc13, and Rab3A interplay in acrosomal exocytosis.
Bello OD, Zanetti MN, Mayorga LS, Michaut MA
Experimental cell research (2012) 3185: 478-88. 126 103 WB, ICC
Non-additive potentiation of glutamate release by phorbol esters and metabotropic mGlu7 receptor in cerebrocortical nerve terminals.
Martín R, Bartolomé-Martín D, Torres M, Sánchez-Prieto J
Journal of neurochemistry (2011) 1164: 476-85. 126 103 ICC, WB; tested species: mouse
A role for the ubiquitin-proteasome system in activity-dependent presynaptic silencing.
Jiang X, Litkowski PE, Taylor AA, Lin Y, Snider BJ, Moulder KL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 305: 1798-809. 126 103 ICC
Dynein light chain regulates axonal trafficking and synaptic levels of Bassoon.
Fejtova A, Davydova D, Bischof F, Lazarevic V, Altrock WD, Romorini S, Schöne C, Zuschratter W, Kreutz MR, Garner CC, Ziv NE, et al.
The Journal of cell biology (2009) 1852: 341-55. 126 103 ICC
Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis.
Leal-Ortiz S, Waites CL, Terry-Lorenzo R, Zamorano P, Gundelfinger ED, Garner CC
The Journal of cell biology (2008) 1815: 831-46. 126 103 ICC
Combinatorial expression of neurexins and LAR-type phosphotyrosine phosphatase receptors instructs assembly of a cerebellar circuit.
Sclip A, Südhof TC
Nature communications (2023) 141: 4976. 126 103 IHC; tested species: mouse
Laser microscopy acquisition and analysis of premotor synapses in the murine spinal cord.
Buettner JM, Kirmann T, Mentis GZ, Hallermann S, Simon CM
STAR protocols (2022) 31: 101236. 126 103 IHC; tested species: mouse
Munc13-1 is a Ca2+-phospholipid-dependent vesicle priming hub that shapes synaptic short-term plasticity and enables sustained neurotransmission.
Lipstein N, Chang S, Lin KH, López-Murcia FJ, Neher E, Taschenberger H, Brose N
Neuron (2021) : . 126 103 WB, IHC; tested species: mouse
Variability in the Munc13-1 content of excitatory release sites.
Karlocai MR, Heredi J, Benedek T, Holderith N, Lorincz A, Nusser Z
eLife (2021) 10: . 126 103 IHC, EM; tested species: mouse
Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue.
Vatan T, Minehart JA, Zhang C, Agarwal V, Yang J, Speer CM
STAR protocols (2021) 24: 100971. 126 103 IHC; tested species: mouse
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 126 103 IHC; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 126 103 IHC; tested species: mouse
Bassoon-disruption slows vesicle replenishment and induces homeostatic plasticity at a CNS synapse.
Mendoza Schulz A, Jing Z, Sánchez Caro JM, Wetzel F, Dresbach T, Strenzke N, Wichmann C, Moser T
The EMBO journal (2014) 335: 512-27. 126 103 IHC
Deficits in morphofunctional maturation of hippocampal mossy fiber synapses in a mouse model of intellectual disability.
Lanore F, Labrousse VF, Szabo Z, Normand E, Blanchet C, Mulle C
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3249: 17882-93. 126 103 IHC
Impaired development of hippocampal mossy fibre synapses in mouse mutants for the presynaptic scaffold protein Bassoon.
Lanore F, Blanchet C, Fejtova A, Pinheiro P, Richter K, Balschun D, Gundelfinger E, Mulle C
The Journal of physiology (2010) 588Pt 12: 2133-45. 126 103 IHC; tested species: mouse
Active zone proteins are dynamically associated with synaptic ribbons in rat pinealocytes.
Spiwoks-Becker I, Maus C, tom Dieck S, Fejtová A, Engel L, Wolloscheck T, Wolfrum U, Vollrath L, Spessert R
Cell and tissue research (2008) 3332: 185-95. 126 103 IHC
Enhanced synaptic protein visualization by multicolor super-resolution expansion microscopy.
Eilts J, Reinhard S, Michetschläger N, Werner C, Sauer M
Neurophotonics (2023) 104: 044412. 126 103 ExM; tested species: mouse
Loss of postsynaptic NMDARs drives nanoscale reorganization of Munc13-1 and PSD-95.
Dharmasri PA, DeMarco EM, Anderson MC, Levy AD, Blanpied TA
bioRxiv : the preprint server for biology (2024) : . 126 103 ICC, DNA-PAINT; tested species: rat
Molecular definition of distinct active zone protein machineries for Ca2+ channel clustering and synaptic vesicle priming.
Emperador-Melero J, Andersen JW, Metzbower SR, Levy AD, Dharmasri PA, de Nola G, Blanpied TA, Kaeser PS
bioRxiv : the preprint server for biology (2023) : . 126 103 ICC, DNA-PAINT; tested species: mouse
Gold In-and-Out: A Toolkit for Analyzing Subcellular Distribution of Immunogold-Labeled Membrane Proteins in Freeze-Fracture Replica Images.
Guerrero-Given D, Goldin SL, Thomas CI, Anthony SA, Jerez D, Kamasawa N
Frontiers in neuroanatomy (2022) 16: 855218. 126 103 EM; tested species: mouse
Different priming states of synaptic vesicles underlie distinct release probabilities at hippocampal excitatory synapses.
Aldahabi M, Balint F, Holderith N, Lorincz A, Reva M, Nusser Z
Neuron (2022) : . 126 103 EM; tested species: mouse
Variability in the Munc13-1 content of excitatory release sites.
Karlocai MR, Heredi J, Benedek T, Holderith N, Lorincz A, Nusser Z
eLife (2021) 10: . 126 103 IHC, EM; tested species: mouse
Distinct Nanoscale Calcium Channel and Synaptic Vesicle Topographies Contribute to the Diversity of Synaptic Function.
Rebola N, Reva M, Kirizs T, Szoboszlay M, Lőrincz A, Moneron G, Nusser Z, DiGregorio DA
Neuron (2019) 1044: 693-710.e9. 126 103 EM; tested species: mouse
Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.
Vasileva M, Horstmann H, Geumann C, Gitler D, Kuner T
The European journal of neuroscience (2012) 368: 3005-20. 126 103 ELISA
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Background
Munc 13s are homologues of the C. elegans unc-13 gene product. Three brain specific isoforms, Munc 13-1, -13-2, and -13-3 are expressed in rat where they localize to presynaptic terminals. All three isoforms share multiple regulatory domains that may mediate phorbol ester and diacylglycerol binding.
Munc13-1 shows the broadest expression pattern and is found in cortex, cerebellum, olfactory bulb and hippocampus. Munc 13-2 is mainly expressed in cortex and hippocampus whereas Munc 13-3 exhibits highest expression levels in cerebellum and pons. Munc13-1 interacts directly with a putative coiled coil domain in the N-terminal part of syntaxin and is involved in synaptic vesicle priming. For Munc13-2 an additional ubiquitously expressed N-terminal splice variant (ubMunc 13-2) has been described.
Munc 13-3 has been shown to be involved in the regulation of cerebellar synaptic transmission and motor learning.
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