Synaptic Systems - Munc13-1

Cat. No.: 126 103

Amount: 50 µg

Price: $480.00

Cat. No. 126 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes (see remarks) ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 200 (see remarks) gallery IHC-P: not tested yet ExM: yes DNA-PAINT: yes EM: ELISA:
Immunogen	Recombinant protein corresponding to AA 3 to 317 from rat Munc13-1 (UniProt Id: Q62768)
Reactivity	Reacts with: human (Q9UPW8), rat (Q62768), mouse (Q4KUS2), zebrafish. Other species not tested yet.
Specificity	K.O. validated PubMed: 28772123
Remarks	IP: For most effective IP, use the denaturing IP-protocol. Consider that protein-protein interactions may be affected. IHC: Heat-mediated antigen retrieval (citrate buffer pH 6) is required for immunohistochemical staining.
Data sheet	126_103.pdf

Liprin-α proteins are master regulators of human presynapse assembly.
Marcó de la Cruz B, Campos J, Molinaro A, Xie X, Jin G, Wei Z, Acuna C, Sterky FH
Nature neuroscience (2024) : . 126 103 WB, ICC; tested species: human

Munc13 supports fusogenicity of non-docked vesicles at synapses with disrupted active zones.
Tan C, de Nola G, Qiao C, Imig C, Born RT, Brose N, Kaeser PS
eLife (2022) 11: . 126 103 WB, ICC; KO verified; tested species: mouse

Bassoon controls synaptic vesicle release via regulation of presynaptic phosphorylation and cAMP.
Montenegro-Venegas C, Guhathakurta D, Pina-Fernandez E, Andres-Alonso M, Plattner F, Gundelfinger ED, Fejtova A
EMBO reports (2022) 238: e53659. 126 103 WB, ICC; tested species: mouse

TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13A.
Brown AL, Wilkins OG, Keuss MJ, Hill SE, Zanovello M, Lee WC, Bampton A, Lee FCY, Masino L, Qi YA, Bryce-Smith S, et al.
Nature (2022) 6037899: 131-137. 126 103 WB; tested species: human

An active vesicle priming machinery suppresses axon regeneration upon adult CNS injury.
Hilton BJ, Husch A, Schaffran B, Lin TC, Burnside ER, Dupraz S, Schelski M, Kim J, Müller JA, Schoch S, Imig C, et al.
Neuron (2021) : . 126 103 WB, ICC; KO verified; tested species: mouse

Munc13-1 is a Ca2+-phospholipid-dependent vesicle priming hub that shapes synaptic short-term plasticity and enables sustained neurotransmission.
Lipstein N, Chang S, Lin KH, López-Murcia FJ, Neher E, Taschenberger H, Brose N
Neuron (2021) : . 126 103 WB, IHC; tested species: mouse

Actions of Rab27B-GTPase on mammalian central excitatory synaptic transmission.
Arias-Hervert ER, Xu N, Njus M, Murphy GG, Hou Y, Williams JA, Lentz SI, Ernst SA, Stuenkel EL
Physiological reports (2020) 89: e14428. 126 103 WB; tested species: mouse

Receptor protein tyrosine phosphatase delta is not essential for synapse maintenance or transmission at hippocampal synapses.
Han KA, Lee HY, Lim D, Shin J, Yoon TH, Liu X, Um JW, Choi SY, Ko J
Molecular brain (2020) 131: 94. 126 103 WB; tested species: mouse

Disentangling the roles of RIM and Munc13 in synaptic vesicle localization and neurotransmission.
Zarebidaki F, Camacho M, Brockmann MM, Trimbuch T, Herman MA, Rosenmund C
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 126 103 WB; KD verified; tested species: mouse

Autaptic cultures of human induced neurons as a versatile platform for studying synaptic function and neuronal morphology.
Fenske P, Grauel MK, Brockmann MM, Dorrn AL, Trimbuch T, Rosenmund C
Scientific reports (2019) 91: 4890. 126 103 WB; KD verified; tested species: human

Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts.
Raja MK, Preobraschenski J, Del Olmo-Cabrera S, Martinez-Turrillas R, Jahn R, Perez-Otano I, Wesseling JF
eLife (2019) 8: . 126 103 WB; tested species: mouse

Postsynaptic RIM1 modulates synaptic function by facilitating membrane delivery of recycling NMDARs in hippocampal neurons.
Wang J, Lv X, Wu Y, Xu T, Jiao M, Yang R, Li X, Chen M, Yan Y, Chen C, Dong W, et al.
Nature communications (2018) 91: 2267. 126 103 WB; tested species: mouse

Inhibitory role of Munc13-1 in antigen-induced mast cell degranulation.
Higashio H, Satoh YI, Saino T
Biomedical research (Tokyo, Japan) (2017) 386: 321-329. 126 103 WB; tested species: rat

Molecular Mechanisms of Synaptic Vesicle Priming by Munc13 and Munc18.
Lai Y, Choi UB, Leitz J, Rhee HJ, Lee C, Altas B, Zhao M, Pfuetzner RA, Wang AL, Brose N, Rhee J, et al.
Neuron (2017) 953: 591-607.e10. 126 103 WB; KO verified; tested species: mouse

Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 126 103 WB; tested species: rat

The Munc13 proteins differentially regulate readily releasable pool dynamics and calcium-dependent recovery at a central synapse.
Chen Z, Cooper B, Kalla S, Varoqueaux F, Young SM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3319: 8336-51. 126 103 WB

β-Adrenergic receptors activate exchange protein directly activated by cAMP (Epac), translocate Munc13-1, and enhance the Rab3A-RIM1α interaction to potentiate glutamate release at cerebrocortical nerve terminals.
Ferrero JJ, Alvarez AM, Ramírez-Franco J, Godino MC, Bartolomé-Martín D, Aguado C, Torres M, Luján R, Ciruela F, Sánchez-Prieto J
The Journal of biological chemistry (2013) 28843: 31370-85. 126 103 WB; tested species: mouse

Different Munc13 isoforms function as priming factors in lytic granule release from murine cytotoxic T lymphocytes.
Dudenhöffer-Pfeifer M, Schirra C, Pattu V, Halimani M, Maier-Peuschel M, Marshall MR, Matti U, Becherer U, Dirks J, Jung M, Lipp P, et al.
Traffic (Copenhagen, Denmark) (2013) 147: 798-809. 126 103 WB

RIM, Munc13, and Rab3A interplay in acrosomal exocytosis.
Bello OD, Zanetti MN, Mayorga LS, Michaut MA
Experimental cell research (2012) 3185: 478-88. 126 103 WB, ICC

Non-additive potentiation of glutamate release by phorbol esters and metabotropic mGlu7 receptor in cerebrocortical nerve terminals.
Martín R, Bartolomé-Martín D, Torres M, Sánchez-Prieto J
Journal of neurochemistry (2011) 1164: 476-85. 126 103 ICC, WB; tested species: mouse

Cat. No.: 126 103

Amount: 50 µg

Price: $480.00

ICC

IHC

Background

Protocols

Munc13-1 antibody - 126 103 K.O.