Synaptic Systems - Munc13-1

Cat. No.: 126 102

Amount: 200 µl

Price: $355.00

Cat. No. 126 102	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes (see remarks) ICC: 1 : 500 gallery IHC: 1 : 200 gallery IHC-P: not tested yet ELISA: (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Immunogen	Recombinant protein corresponding to AA 3 to 317 from rat Munc13-1 (UniProt Id: Q62768)
Reactivity	Reacts with: human (Q9UPW8), rat (Q62768), mouse (Q4KUS2), zebrafish. Other species not tested yet.
Specificity	K.O. validated
Remarks	IP: For most effective IP, use the denaturing IP-protocol. Consider that protein-protein interactions may be affected. ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 126 111 as capture antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet	126_102.pdf

References for Munc13-1 - 126 102

cAMP-EPAC-PKCε-RIM1α signaling regulates presynaptic long-term potentiation and motor learning.
Wang XT, Zhou L, Dong BB, Xu FX, Wang DJ, Shen EW, Cai XY, Wang Y, Wang N, Ji SJ, Chen W, et al.
eLife (2023) 12: . 126 102 WB; tested species: mouse

A presynaptic phosphosignaling hub for lasting homeostatic plasticity.
Müller JA, Betzin J, Santos-Tejedor J, Mayer A, Oprişoreanu AM, Engholm-Keller K, Paulußen I, Gulakova P, McGovern TD, Gschossman LJ, Schönhense E, et al.
Cell reports (2022) 393: 110696. 126 102 WB; tested species: mouse

Presynaptic short-term plasticity persists in the absence of PKC phosphorylation of Munc18-1.
Wang CC, Weyrer C, Fioravante D, Kaeser PS, Regehr WG
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) : . 126 102 WB; tested species: mouse

No symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington's disease.
Huang TT, Smith R, Bacos K, Song DY, Faull RM, Waldvogel HJ, Li JY
Acta neuropathologica communications (2020) 81: 77. 126 102 WB, ICC; tested species: mouse

Disabling the Gβγ-SNARE interaction disrupts GPCR-mediated presynaptic inhibition, leading to physiological and behavioral phenotypes.
Zurawski Z, Thompson Gray AD, Brady LJ, Page B, Church E, Harris NA, Dohn MR, Yim YY, Hyde K, Mortlock DP, Jones CK, et al.
Science signaling (2019) 12569: . 126 102 WB; tested species: mouse

SNARE Complex-associated Proteins in the Lateral Amygdala of Macaca mullatta Following Long-term Ethanol Drinking.
Alexander NJ, Rau AR, Jimenez VA, Daunais JB, Grant KA, McCool BA
Alcoholism, clinical and experimental research (2018) : . 126 102 WB; tested species: monkey

Spinal TNF-α impedes Fbxo45-dependent Munc13-1 ubiquitination to mediate neuropathic allodynia in rats.
Hsieh MC, Ho YC, Lai CY, Chou D, Chen GD, Lin TB, Peng HY
Cell death & disease (2018) 98: 811. 126 102 WB, IHC; KD verified; tested species: rat

Ethanol Mediated Inhibition of Synaptic Vesicle Recycling at Amygdala Glutamate Synapses Is Dependent upon Munc13-2.
Gioia DA, Alexander N, McCool BA
Frontiers in neuroscience (2017) 11: 424. 126 102 WB; tested species: mouse

Differential Expression of Munc13-2 Produces Unique Synaptic Phenotypes in the Basolateral Amygdala of C57BL/6J and DBA/2J Mice.
Gioia DA, Alexander NJ, McCool BA
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3643: 10964-10977. 126 102 WB

Activity-Dependent Degradation of Synaptic Vesicle Proteins Requires Rab35 and the ESCRT Pathway.
Sheehan P, Zhu M, Beskow A, Vollmer C, Waites CL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3633: 8668-86. 126 102 WB; tested species: rat

Epac2 Mediates cAMP-Dependent Potentiation of Neurotransmission in the Hippocampus.
Fernandes HB, Riordan S, Nomura T, Remmers CL, Kraniotis S, Marshall JJ, Kukreja L, Vassar R, Contractor A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3516: 6544-53. 126 102 WB

Active zone protein expression changes at the key stages of cerebellar cortex neurogenesis in the rat.
Juranek JK, Mukherjee K, Siddiqui TJ, Kaplan BJ, Li JY, Ahnert-Hilger G, Jahn R, Calka J
Acta histochemica (2013) 1156: 616-25. 126 102 WB, ICC, IHC

Neuronal BIN1 Regulates Presynaptic Neurotransmitter Release and Memory Consolidation.
De Rossi P, Nomura T, Andrew RJ, Masse NY, Sampathkumar V, Musial TF, Sudwarts A, Recupero AJ, Le Metayer T, Hansen MT, Shim HN, et al.
Cell reports (2020) 3010: 3520-3535.e7. 126 102 IHC; tested species: mouse

RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses.
Brockmann MM, Maglione M, Willmes CG, Stumpf A, Bouazza BA, Velasquez LM, Grauel MK, Beed P, Lehmann M, Gimber N, Schmoranzer J, et al.
eLife (2019) 8: . 126 102 IHC; tested species: mouse

Ubiquitination acutely regulates presynaptic neurotransmitter release in mammalian neurons.
Rinetti GV, Schweizer FE
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 309: 3157-66. 126 102 IHC

Cat. No.: 126 102

Amount: 200 µl

Price: $355.00

ICC

IHC

Background

Munc 13s are homologues of the C. elegans unc-13 gene product. Three brain specific isoforms, Munc 13-1, -13-2, and -13-3 are expressed in rat where they localize to presynaptic terminals. All three isoforms share multiple regulatory domains that may mediate phorbol ester and diacylglycerol binding.
Munc13-1 shows the broadest expression pattern and is found in cortex, cerebellum, olfactory bulb and hippocampus. Munc 13-2 is mainly expressed in cortex and hippocampus whereas Munc 13-3 exhibits highest expression levels in cerebellum and pons. Munc13-1 interacts directly with a putative coiled coil domain in the N-terminal part of syntaxin and is involved in synaptic vesicle priming. For Munc13-2 an additional ubiquitously expressed N-terminal splice variant (ubMunc 13-2) has been described.
Munc 13-3 has been shown to be involved in the regulation of cerebellar synaptic transmission and motor learning.

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Protocols

ICC

IHC

Other products for this target

Munc13-1 antibody - 126 102 K.O.

References for Munc13-1 - 126 102