Synaptic Systems

Cat. No.: HS-427 017

Amount: 200 µl

Price: $415.00

Cell-programmed nutrient partitioning in the tumour microenvironment.
Reinfeld BI, Madden MZ, Wolf MM, Chytil A, Bader JE, Patterson AR, Sugiura A, Cohen AS, Ali A, Do BT, Muir A, et al.
Nature (2021) 5937858: 282-288. HS-427 017 IHC-P; tested species: mouse

IHC

IHC-P

IHC-Fr

Background

CD45, also designated as Leukocyte Common Antigen (LCA) or protein tyrosine phosphatase receptor type C (PTPRC), is a type I transmembrane protein expressed on all nucleated cells of the hematopoietic system, except mature erythrocytes and platelets (1). Several isoforms of CD45 have been identified which are generated by differential splicing of exons 4, 5, and 6, thereby generating the CD45RA, RB and RC isoforms, respectively (2). Expression of the isoforms differs according to cell type and functional status. The CD45RABC (B220) long isoform is almost exclusively expressed on B cells (3). Naïve human T cells express the high molecular weight isoform CD45RA. Activation of T cells and differentiation to memory T cells is accompanied by exon exclusion for production of the short isoform CD45RO (3). CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. Thus, CD45-deficient humans and mice develop a severe-combined immunodeficiency (SCID) phenotype (4). In macrophages, CD45 plays a central role in their adhesion, morphology and motility (5).

1 ) Trowbridge IS et al., Annu. Rev. Immunol. (1994)
2 ) Birkeland ML et al., J. Mol. Cell. Immunol. (1988)
3 ) Ledbetter JA et al., Proc. Natl. Acad. Sci. U.S.A. (1988)
4 ) Hermiston ML et al., Annu Rev Immunol (2003)
5 ) McNeill L et al., Immunol. Lett. (2004)

Other products for this target

Cat. No. HS-427 017	200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H₂O. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: not tested yet IHC: 1 : 100 gallery IHC-P: 1 : 100 gallery IHC-Fr: 1 : 100 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	GHH45/97A2
Subtype	IgG2b
Immunogen	Synthetic peptide corresponding to AA 1284 to 1291 from mouse PTPRC (UniProt Id: P06800-1)
Reactivity	Reacts with: mouse (P06800-1). No signal: human, rat. Other species not tested yet.
Specificity	The antibody recognizes all CD45 isoforms.
Remarks	IHC-Fr: MeOH fixation is recommended.
Data sheet	hs-427_017.pdf

CD45 antibody mouse specific - HS-427 017

References for CD45 - HS-427 017