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Home > Products > 405 003

Histidine Decarboxylase antibody - 405 003

Histidine decarboxylase is an enzyme involved in histamine synthesis
Rabbit polyclonal purified antibody
Cat. No.: 405 003
Amount: 50 µg
Price: $375.00
Cat. No. 405 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P: 1 : 200 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1 to 658 from rat HDC (UniProt Id: P16453)
Reactivity Reacts with: mouse (P23738), rat (P16453).
Other species not tested yet.
Data sheet 405_003.pdf

References for Histidine Decarboxylase - 405 003

Structural Basis for Histaminergic Regulation of Neural Circuits in the Mouse Olfactory Bulb.
Minami-Ogawa Y, Kiyokage E, Yamanishi H, Horie S, Ichikawa S, Toida K
The Journal of comparative neurology (2024) 53210: e25671. 405 003 IHC, EM; tested species: mouse
Structural Basis for Histaminergic Regulation of Neural Circuits in the Mouse Olfactory Bulb.
Minami-Ogawa Y, Kiyokage E, Yamanishi H, Horie S, Ichikawa S, Toida K
The Journal of comparative neurology (2024) 53210: e25671. 405 003 IHC, EM; tested species: mouse
Cat. No.: 405 003
Amount: 50 µg
Price: $375.00
Structural Basis for Histaminergic Regulation of Neural Circuits in the Mouse Olfactory Bulb.
Minami-Ogawa Y, Kiyokage E, Yamanishi H, Horie S, Ichikawa S, Toida K
The Journal of comparative neurology (2024) 53210: e25671. 405 003 IHC, EM; tested species: mouse
Structural Basis for Histaminergic Regulation of Neural Circuits in the Mouse Olfactory Bulb.
Minami-Ogawa Y, Kiyokage E, Yamanishi H, Horie S, Ichikawa S, Toida K
The Journal of comparative neurology (2024) 53210: e25671. 405 003 IHC, EM; tested species: mouse
IHC
IHC-P
Background

Histamine is synthesized by histidine decarboxylase (HDC) in many different cell types. Mast cells and basopile granulocytes store histamine in their granules that is released upon external stimulation.
In addition histamine acts as a modulatory neurotransmitter in the mammalian brain.
HDC expressing neurons occur in the tuberomammillary nucleus (TMN), which is located in the hypothalamus. It is the only site of neuronal histamine synthesis in the adult mammalian brain.