Cat. No. 132 003CpH |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with CypHer5E.
CypHer5E® is a pH-sensitive dye, fluorescent only after antibody internalization at the acidic pH of the lumen of the synaptic vesicle. CypHer5E® can be detected with the filter sets for Cy5®. This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Application Number US 2006-0051758 and related patents. Cy, CyDye, and CypHer are trademarks of GE-Healthcare Ltd. Purchase of reagents related to the CypHer technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from GE-Healthcare Bio-Science Corp., 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: N/A Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 100 up to 1 : 1000 gallery Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: not tested yet Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: not tested yet |
Label | CypHer5E |
Immunogen | Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212) |
Specificity | Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants.Does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives. |
Remarks |
This antibody is suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment. |
Data sheet | 132_003cph.pdf |