Tailor-made Antibodies
and Tools for Life Science

Gephyrin antibody - 147 021 K.O.

Gephyrin is a scaffolding protein associated with inhibitory neurotransmitter receptors
Mouse monoclonal hybridoma supernatant
Cat. No.: 147 021
Amount: 300 µl
Price: $440.00
Cat. No. 147 021 300 µl hybridoma supernatant, lyophilized. For reconstitution add 300 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended (see remarks)
IP: not recommended (see remarks)
ICC: 1 : 250 up to 1 : 500 gallery  
IHC: 1 : 100 up to 1 : 250 (see remarks) gallery  
IHC-P: not recommended
IHC-Fr: 1 : 500 (see remarks) gallery  
IHC-G: yes
ExM: yes
EM: yes
Clone mAb7a
Subtype IgG1 (κ light chain)
Immunogen Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Epitop AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reactivity Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish, frog.
Other species not tested yet.
Specificity Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O. validated
Remarks

WB: Clone 3B11 (cat. no. 147 111) is highly recommended.
IP: Clone 3B11 (cat. no. 147 111) highly recommended.
IHC: Hybridoma supernatant highly recommended. For best results use the protocol of Schneider Gasser et al., 2006.
Alternatively antigen retrieval (10 mM citrate, pH 6.0, over night at 60°C) can be applied.

Glyoxal fixation according to Konno et al. 2023 has improved signal quality and strength.
IHC-Fr: Fixation with acetone or PFA/formaldehyde is recommended. Postfixation with methanol is not advised.

Data sheet 147_021.pdf

References for Gephyrin - 147 021

Sleep and wake cycles dynamically modulate hippocampal inhibitory synaptic plasticity.
Wu K, Han W, Lu W
PLoS biology (2022) 2011: e3001812. 147 021 WB; tested species: mouse
Interference With Complex IV as a Model of Age-Related Decline in Synaptic Connectivity.
Kriebel M, Ebel J, Battke F, Griesbach S, Volkmer H
Frontiers in molecular neuroscience (2020) 13: 43. 147 021 WB, ICC; tested species: rat
Expression of Neurofilament Subunits at Neocortical Glutamatergic and GABAergic Synapses.
Bragina L, Conti F
Frontiers in neuroanatomy (2018) 12: 74. 147 021 WB, IHC; tested species: rat
Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development.
Li J, Han W, Pelkey KA, Duan J, Mao X, Wang YX, Craig MT, Dong L, Petralia RS, McBain CJ, Lu W, et al.
Neuron (2017) 964: 808-826.e8. 147 021 WB, ICC, IHC; tested species: mouse
Cat. No.: 147 021
Amount: 300 µl
Price: $440.00