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Home > Products > 147 018

Gephyrin antibody - 147 018 K.O.

Gephyrin is a scaffolding protein associated with inhibitory neurotransmitter receptors
Rabbit monoclonal recombinant IgG supernatant
Cat. No.: 147 018
Amount: 200 µl
Price: $415.00
Cat. No. 147 018 200 µl purified recombinant IgG supernatant, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended (see remarks)
IP: not recommended (see remarks)
ICC: 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC-P: not recommended
Clone RbmAb7a
Subtype IgG1 (κ light chain)
Immunogen Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Epitop N-terminus of gephyrin, surrounding phospho-serines 268 and 270.
Reactivity Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish.
Other species not tested yet.
Specificity Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O. validated
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb7a. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents.
WB: Clone 3B11 (cat. no. 147 111) is highly recommended.
IP: Clone 3B11 (cat. no. 147 111) is highly recommended.
IHC: For best results use the protocol of Schneider Gasser et al., 2006.
Alternatively antigen retrieval (10 mM citrate, pH 6.0, over night at 60°C) can be applied.
Data sheet 147_018.pdf

References for Gephyrin - 147 018

ErbB4 promotes inhibitory synapse formation by cell adhesion, independent of its kinase activity.
Luo B, Liu Z, Lin D, Chen W, Ren D, Yu Z, Xiong M, Zhao C, Fei E, Li B
Translational psychiatry (2021) 111: 361. 147 018 WB, ICC; tested species: mouse
ErbB4 promotes inhibitory synapse formation by cell adhesion, independent of its kinase activity.
Luo B, Liu Z, Lin D, Chen W, Ren D, Yu Z, Xiong M, Zhao C, Fei E, Li B
Translational psychiatry (2021) 111: 361. 147 018 WB, ICC; tested species: mouse
Surfaceome dynamics reveal proteostasis-independent reorganization of neuronal surface proteins during development and synaptic plasticity.
van Oostrum M, Campbell B, Seng C, Müller M, Tom Dieck S, Hammer J, Pedrioli PGA, Földy C, Tyagarajan SK, Wollscheid B
Nature communications (2020) 111: 4990. 147 018 ICC; tested species: rat
A Conserved Tyrosine Residue in Slitrk3 Carboxyl-Terminus Is Critical for GABAergic Synapse Development.
Li J, Han W, Wu K, Li YD, Liu Q, Lu W
Frontiers in molecular neuroscience (2019) 12: 213. 147 018 ICC; tested species: mouse
miR-143-3p modulates depressive-like behaviors via Lasp1 in the mouse ventral hippocampus.
Yu H, Li X, Zhang Q, Geng L, Su B, Wang Y
Communications biology (2024) 71: 944. 147 018 IHC; tested species: mouse
Excitation-inhibition imbalance disrupts visual familiarity in amyloid and non-pathology conditions.
Niraula S, Doderer JJ, Indulkar S, Berry KP, Hauser WL, L'Esperance OJ, Deng JZ, Keeter G, Rouse AG, Subramanian J
Cell reports (2023) 421: 111946. 147 018 IHC; tested species: mouse
Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging.
Britton R, Wasley T, Harish R, Holz C, Hall J, Yee DC, Melton Witt J, Booth EA, Braithwaite S, Czirr E, Kerrisk Campbell M, et al.
eNeuro (2023) 106: . 147 018 IHC; tested species: mouse
Reprogramming reactive glia into interneurons reduces chronic seizure activity in a mouse model of mesial temporal lobe epilepsy.
Lentini C, d'Orange M, Marichal N, Trottmann MM, Vignoles R, Foucault L, Verrier C, Massera C, Raineteau O, Conzelmann KK, Rival-Gervier S, et al.
Cell stem cell (2021) : . 147 018 IHC; tested species: mouse
ROCK/PKA inhibition rescues hippocampal hyperexcitability and GABAergic neuron alterations in Oligophrenin-1 Knock-out mouse model of X-linked intellectual disability.
Busti I, Allegra M, Spalletti C, Panzi C, Restani L, Billuart P, Caleo M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 147 018 IHC; tested species: mouse
Spinal Wnt5a Plays a Key Role in Spinal Dendritic Spine Remodeling in Neuropathic and Inflammatory Pain Models and in the Proalgesic Effects of Peripheral Wnt3a.
Simonetti M, Kuner R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4035: 6664-6677. 147 018 IHC; tested species: mouse
Cat. No.: 147 018
Amount: 200 µl
Price: $415.00
ErbB4 promotes inhibitory synapse formation by cell adhesion, independent of its kinase activity.
Luo B, Liu Z, Lin D, Chen W, Ren D, Yu Z, Xiong M, Zhao C, Fei E, Li B
Translational psychiatry (2021) 111: 361. 147 018 WB, ICC; tested species: mouse
ErbB4 promotes inhibitory synapse formation by cell adhesion, independent of its kinase activity.
Luo B, Liu Z, Lin D, Chen W, Ren D, Yu Z, Xiong M, Zhao C, Fei E, Li B
Translational psychiatry (2021) 111: 361. 147 018 WB, ICC; tested species: mouse
Surfaceome dynamics reveal proteostasis-independent reorganization of neuronal surface proteins during development and synaptic plasticity.
van Oostrum M, Campbell B, Seng C, Müller M, Tom Dieck S, Hammer J, Pedrioli PGA, Földy C, Tyagarajan SK, Wollscheid B
Nature communications (2020) 111: 4990. 147 018 ICC; tested species: rat
A Conserved Tyrosine Residue in Slitrk3 Carboxyl-Terminus Is Critical for GABAergic Synapse Development.
Li J, Han W, Wu K, Li YD, Liu Q, Lu W
Frontiers in molecular neuroscience (2019) 12: 213. 147 018 ICC; tested species: mouse
miR-143-3p modulates depressive-like behaviors via Lasp1 in the mouse ventral hippocampus.
Yu H, Li X, Zhang Q, Geng L, Su B, Wang Y
Communications biology (2024) 71: 944. 147 018 IHC; tested species: mouse
Excitation-inhibition imbalance disrupts visual familiarity in amyloid and non-pathology conditions.
Niraula S, Doderer JJ, Indulkar S, Berry KP, Hauser WL, L'Esperance OJ, Deng JZ, Keeter G, Rouse AG, Subramanian J
Cell reports (2023) 421: 111946. 147 018 IHC; tested species: mouse
Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging.
Britton R, Wasley T, Harish R, Holz C, Hall J, Yee DC, Melton Witt J, Booth EA, Braithwaite S, Czirr E, Kerrisk Campbell M, et al.
eNeuro (2023) 106: . 147 018 IHC; tested species: mouse
Reprogramming reactive glia into interneurons reduces chronic seizure activity in a mouse model of mesial temporal lobe epilepsy.
Lentini C, d'Orange M, Marichal N, Trottmann MM, Vignoles R, Foucault L, Verrier C, Massera C, Raineteau O, Conzelmann KK, Rival-Gervier S, et al.
Cell stem cell (2021) : . 147 018 IHC; tested species: mouse
ROCK/PKA inhibition rescues hippocampal hyperexcitability and GABAergic neuron alterations in Oligophrenin-1 Knock-out mouse model of X-linked intellectual disability.
Busti I, Allegra M, Spalletti C, Panzi C, Restani L, Billuart P, Caleo M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 147 018 IHC; tested species: mouse
Spinal Wnt5a Plays a Key Role in Spinal Dendritic Spine Remodeling in Neuropathic and Inflammatory Pain Models and in the Proalgesic Effects of Peripheral Wnt3a.
Simonetti M, Kuner R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4035: 6664-6677. 147 018 IHC; tested species: mouse
ICC
IHC
Background

Gephyrin is a bifunctional protein which is essential for both synaptic clustering of inhibitory neurotransmitter receptors in the central nervous system and the biosynthesis of the molybdenum cofactor (MoCo) in peripheral tissues. It co-purifies with the inhibitory glycine receptor (GlyR) and is expressed abundantly in all brain areas which contain synapses.
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