Synaptic Systems - Gephyrin

Cat. No.: 147 018

Amount: 200 µl

Price: $415.00

Cat. No. 147 018	200 µl purified recombinant IgG supernatant, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended (see remarks) IP: not recommended (see remarks) ICC: 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery IHC-P: not recommended Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	RbmAb7a
Subtype	IgG1 (κ light chain)
Immunogen	Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Reactivity	Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish. Other species not tested yet.
Specificity	Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O. validated
Remarks	This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb7a. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. WB: Clone 3B11 (cat. no. 147 111) is highly recommended. IP: Clone 3B11 (cat. no. 147 111) is highly recommended. IHC: For best results use the protocol of Schneider Gasser et al., 2006. Alternatively antigen retrieval (10 mM citrate, pH 6.0, over night at 60°C) can be applied.
Data sheet	147_018.pdf

References for Gephyrin - 147 018

ErbB4 promotes inhibitory synapse formation by cell adhesion, independent of its kinase activity.
Luo B, Liu Z, Lin D, Chen W, Ren D, Yu Z, Xiong M, Zhao C, Fei E, Li B
Translational psychiatry (2021) 111: 361. 147 018 WB, ICC; tested species: mouse

Surfaceome dynamics reveal proteostasis-independent reorganization of neuronal surface proteins during development and synaptic plasticity.
van Oostrum M, Campbell B, Seng C, Müller M, Tom Dieck S, Hammer J, Pedrioli PGA, Földy C, Tyagarajan SK, Wollscheid B
Nature communications (2020) 111: 4990. 147 018 ICC; tested species: rat

A Conserved Tyrosine Residue in Slitrk3 Carboxyl-Terminus Is Critical for GABAergic Synapse Development.
Li J, Han W, Wu K, Li YD, Liu Q, Lu W
Frontiers in molecular neuroscience (2019) 12: 213. 147 018 ICC; tested species: mouse

miR-143-3p modulates depressive-like behaviors via Lasp1 in the mouse ventral hippocampus.
Yu H, Li X, Zhang Q, Geng L, Su B, Wang Y
Communications biology (2024) 71: 944. 147 018 IHC; tested species: mouse

Excitation-inhibition imbalance disrupts visual familiarity in amyloid and non-pathology conditions.
Niraula S, Doderer JJ, Indulkar S, Berry KP, Hauser WL, L'Esperance OJ, Deng JZ, Keeter G, Rouse AG, Subramanian J
Cell reports (2023) 421: 111946. 147 018 IHC; tested species: mouse

Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging.
Britton R, Wasley T, Harish R, Holz C, Hall J, Yee DC, Melton Witt J, Booth EA, Braithwaite S, Czirr E, Kerrisk Campbell M, et al.
eNeuro (2023) 106: . 147 018 IHC; tested species: mouse

Reprogramming reactive glia into interneurons reduces chronic seizure activity in a mouse model of mesial temporal lobe epilepsy.
Lentini C, d'Orange M, Marichal N, Trottmann MM, Vignoles R, Foucault L, Verrier C, Massera C, Raineteau O, Conzelmann KK, Rival-Gervier S, et al.
Cell stem cell (2021) : . 147 018 IHC; tested species: mouse

ROCK/PKA inhibition rescues hippocampal hyperexcitability and GABAergic neuron alterations in Oligophrenin-1 Knock-out mouse model of X-linked intellectual disability.
Busti I, Allegra M, Spalletti C, Panzi C, Restani L, Billuart P, Caleo M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 147 018 IHC; tested species: mouse

Spinal Wnt5a Plays a Key Role in Spinal Dendritic Spine Remodeling in Neuropathic and Inflammatory Pain Models and in the Proalgesic Effects of Peripheral Wnt3a.
Simonetti M, Kuner R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4035: 6664-6677. 147 018 IHC; tested species: mouse

Cat. No.: 147 018

Amount: 200 µl

Price: $415.00

ICC

IHC

Background

Gephyrin is a bifunctional protein which is essential for both synaptic clustering of inhibitory neurotransmitter receptors in the central nervous system and the biosynthesis of the molybdenum cofactor (MoCo) in peripheral tissues. It co-purifies with the inhibitory glycine receptor (GlyR) and is expressed abundantly in all brain areas which contain synapses.

1 ) Hermann A et al., Biochem. Biophys. Res. Commun. (2001)
2 ) Kirsch J et al., Brain Res. (1993)
3 ) Kirsch J et al., Eur. J. Neurosci. (1993)

Protocols

ICC

IHC

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Gephyrin antibody - 147 018 K.O.

References for Gephyrin - 147 018