Synaptic Systems - GalphaO1/2

Cat. No.: 271 111

Amount: 100 µg

Price: $415.00

Cat. No. 271 111	100 µg purified IgG, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 2000 gallery IHC-P: 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	101.1
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 355 from rat GαO2 (UniProt Id: P18872)
Reactivity	Reacts with: rat (P59215), mouse (P18872), zebrafish. Other species not tested yet.
Specificity	Recognizes GαO 1 and GαO 2. K.O. validated PubMed: 15872115
Data sheet	271_111.pdf

References for GalphaO1/2 - 271 111

Gαo1 and Gαo1/Gαo2 deletion differentially affect hippocampal mossy fiber tract anatomy and neuronal morphogenesis.
Höltje M, Wolkowicz A, Brunk I, Baron J, Ahnert-Hilger G
Journal of neurochemistry (2024) : . 271 111 WB, ICC, IHC; KO verified; tested species: mouse

Balance of Go1α and Go2α expression regulates motor function via the striatal dopaminergic system.
Baron J, Bilbao A, Hörtnagl H, Birnbaumer L, Leixner S, Spanagel R, Ahnert-Hilger G, Brunk I
Journal of neurochemistry (2018) : . 271 111 WB; tested species: mouse

Galphao2 regulates vesicular glutamate transporter activity by changing its chloride dependence.
Winter S, Brunk I, Walther DJ, Höltje M, Jiang M, Peter JU, Takamori S, Jahn R, Birnbaumer L, Ahnert-Hilger G
The Journal of neuroscience : the official journal of the Society for Neuroscience (2005) 2518: 4672-80. 271 111 WB; KO verified

The α-subunit of the trimeric GTPase Go2 regulates axonal growth.
Baron J, Blex C, Rohrbeck A, Rachakonda SK, Birnbaumer L, Ahnert-Hilger G, Brunk I
Journal of neurochemistry (2013) 1246: 782-94. 271 111 ICC

Cat. No.: 271 111

Amount: 100 µg

Price: $415.00

ICC

IHC

IHC-P

Background

Neurotransmitters like monoamines, acetylcholine, glutamate, GABA, and glycine, are loaded into synaptic vesicles by transmitter specific vesicular transporters.
It has been shown that the monoamine transporters VmaT 1 and VmaT 2 are under negative regulation by different α subunits of trimeric G-proteins.
In contrast, the vesicular glutamate transporters (VGLUTs) are exclusively regulated by GαO 2 which modulates the chloride dependence of these proteins.

1 ) Tang G et al., Proc. Natl. Acad. Sci. U.S.A. (2012)
2 ) Höltje M et al., J. Neurosci. (2000)
3 ) Ahnert-Hilger G et al., EMBO J. (1998)

Protocols

Standard protocol

ICC

IHC

IHC-P

GalphaO1/2 antibody - 271 111 K.O.

References for GalphaO1/2 - 271 111