Synaptic Systems

Cat. No.: 174 203

Amount: 50 µg

Price: $375.00

Cat. No. 174 203	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP-staining) gallery IP: not tested yet ICC: 1 : 100 up to 1 : 500 gallery IHC: 1 : 200 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 403 from rat Doc2a (UniProt Id: P70611)
Reactivity	Reacts with: rat (P70611, P70610), mouse (Q7TNF0, P70169). No signal: zebrafish. Other species not tested yet.
Specificity	Detects preferentially Doc 2a with some cross-reactivity to Doc 2b.
Data sheet	174_203.pdf

References for Doc2a/b - 174 203

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 174 203 WB, ICC, IHC; tested species: mouse,rat

A dual function for Munc-18 in exocytosis of PC12 cells.
Schütz D, Zilly F, Lang T, Jahn R, Bruns D
The European journal of neuroscience (2005) 219: 2419-32. 174 203 WB

Trafficking proteins show limited differences in mobility across different postsynaptic spines.
Mougios N, Opazo F, Rizzoli SO, Reshetniak S
iScience (2023) 262: 105971. 174 203 ICC; tested species: rat

Cat. No.: 174 203

Amount: 50 µg

Price: $375.00

A dual function for Munc-18 in exocytosis of PC12 cells.
Schütz D, Zilly F, Lang T, Jahn R, Bruns D
The European journal of neuroscience (2005) 219: 2419-32. 174 203 WB

ICC

IHC

Background

The Doc 2 protein family comprises the three isoforms Doc 2a, b and c. All three proteins are composed of a short N-terminal Munc 13 binding region followed by two C-terminal calcium binding domains (C2A and C2B). Doc 2a and b are predominantly expressed in brain and colocalize with small secretory vesicles whereas Doc 2c is mainly expressed in the heart.
The exact mechanism how the Doc proteins are involved in Ca²⁺ induced exocytosis has yet to be determined.

1 ) Groffen AJ et al., J. Neurochem. (2006)
2 ) Groffen AJ et al., J. Biol. Chem. (2004)
3 ) Korteweg N et al., Eur. J. Neurosci. (2000)
4 ) Duncan RR et al., J. Biol. Chem. (1999)
5 ) Charvin N et al., Biochem. J. (1999)
6 ) Mochida S et al., Proc. Natl. Acad. Sci. U.S.A. (1998)
7 ) Orita S et al., J. Biol. Chem. (1997)
8 ) Verhage M et al., Neuron (1997)
9 ) Kojima T et al., J. Biochem. (1996)
10 ) Orita S et al., Biochem. Biophys. Res. Commun. (1995)

Protocols

ICC

IHC

Doc2a/b antibody - 174 203

References for Doc2a/b - 174 203