Synaptic Systems - Complexin1/2

Cat. No.: 122 002

Amount: 200 µl

Price: $355.00

Cat. No. 122 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery IP: yes ICC: 1 : 1000 gallery IHC: yes gallery IHC-P: 1 : 200 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 122 to 134 from human Complexin2 (UniProt Id: Q6PUV4)
Reactivity	Reacts with: human (O14810, Q6PUV4), rat (P63041, P84087), mouse (P63040, P84086), cow, electric ray, rabbit. Other species not tested yet.
Specificity	Recognizes complexin 1 and 2. K.O. validated PubMed: 11163241
Matching control protein/peptide	122-0P
Data sheet	122_002.pdf

References for Complexin1/2 - 122 002

VAMP4 maintains a Ca2+-sensitive pool of spontaneously recycling synaptic vesicles.
Lin PY, Chanaday NL, Horvath PM, Ramirez DMO, Monteggia LM, Kavalali ET
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 122 002 WB; tested species: rat

Acute Complexin Knockout Abates Spontaneous and Evoked Transmitter Release.
López-Murcia FJ, Reim K, Jahn O, Taschenberger H, Brose N
Cell reports (2019) 2610: 2521-2530.e5. 122 002 WB, ICC; KO verified; tested species: mouse

Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts.
Raja MK, Preobraschenski J, Del Olmo-Cabrera S, Martinez-Turrillas R, Jahn R, Perez-Otano I, Wesseling JF
eLife (2019) 8: . 122 002 WB; tested species: mouse

Disabling the Gβγ-SNARE interaction disrupts GPCR-mediated presynaptic inhibition, leading to physiological and behavioral phenotypes.
Zurawski Z, Thompson Gray AD, Brady LJ, Page B, Church E, Harris NA, Dohn MR, Yim YY, Hyde K, Mortlock DP, Jones CK, et al.
Science signaling (2019) 12569: . 122 002 WB; tested species: mouse

Synaptotagmin 1 clamps synaptic vesicle fusion in mammalian neurons independent of complexin.
Courtney NA, Bao H, Briguglio JS, Chapman ER
Nature communications (2019) 101: 4076. 122 002 WB, ICC; KD verified; tested species: mouse

SNARE Complex-associated Proteins in the Lateral Amygdala of Macaca mullatta Following Long-term Ethanol Drinking.
Alexander NJ, Rau AR, Jimenez VA, Daunais JB, Grant KA, McCool BA
Alcoholism, clinical and experimental research (2018) : . 122 002 WB; tested species: monkey

miR-135a Regulates Synaptic Transmission and Anxiety-Like Behavior in Amygdala.
Mannironi C, Biundo A, Rajendran S, De Vito F, Saba L, Caioli S, Zona C, Ciotti T, Caristi S, Perlas E, Del Vecchio G, et al.
Molecular neurobiology (2017) : . 122 002 WB, ICC; tested species: mouse

Complexin stabilizes newly primed synaptic vesicles and prevents their premature fusion at the mouse calyx of held synapse.
Chang S, Reim K, Pedersen M, Neher E, Brose N, Taschenberger H
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3521: 8272-90. 122 002 WB, IHC

Synaptotagmin-1 and -7 Are Redundantly Essential for Maintaining the Capacity of the Readily-Releasable Pool of Synaptic Vesicles.
Bacaj T, Wu D, Burré J, Malenka RC, Liu X, Südhof TC
PLoS biology (2015) 1310: e1002267. 122 002 WB

Synaptic function of nicastrin in hippocampal neurons.
Lee SH, Sharma M, Südhof TC, Shen J
Proceedings of the National Academy of Sciences of the United States of America (2014) 11124: 8973-8. 122 002 WB; tested species: mouse

Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP.
Kaeser-Woo YJ, Younts TJ, Yang X, Zhou P, Wu D, Castillo PE, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3323: 9769-80. 122 002 WB

Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity.
Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, et al.
The Journal of biological chemistry (2013) 28848: 34906-19. 122 002 WB, IP; tested species: mouse

CSPα knockout causes neurodegeneration by impairing SNAP-25 function.
Sharma M, Burré J, Bronk P, Zhang Y, Xu W, Südhof TC
The EMBO journal (2012) 314: 829-41. 122 002 WB; tested species: mouse

Complexin 2 modulates vesicle-associated membrane protein (VAMP) 2-regulated zymogen granule exocytosis in pancreatic acini.
Falkowski MA, Thomas DD, Groblewski GE
The Journal of biological chemistry (2010) 28546: 35558-66. 122 002 WB, ICC

Complexins facilitate neurotransmitter release at excitatory and inhibitory synapses in mammalian central nervous system.
Xue M, Stradomska A, Chen H, Brose N, Zhang W, Rosenmund C, Reim K
Proceedings of the National Academy of Sciences of the United States of America (2008) 10522: 7875-80. 122 002 WB; KO verified; tested species: mouse

Structurally and functionally unique complexins at retinal ribbon synapses.
Reim K, Wegmeyer H, Brandstätter JH, Xue M, Rosenmund C, Dresbach T, Hofmann K, Brose N
The Journal of cell biology (2005) 1694: 669-80. 122 002 WB, IHC

Complexins regulate a late step in Ca2+-dependent neurotransmitter release.
Reim K, Mansour M, Varoqueaux F, McMahon HT, Südhof TC, Brose N, Rosenmund C
Cell (2001) 1041: 71-81. 122 002 WB; KO verified; tested species: mouse

Compartmentalization of soluble endocytic proteins in synaptic vesicle clusters by phase separation.
Yoshida T, Takenaka KI, Sakamoto H, Kojima Y, Sakano T, Shibayama K, Nakamura K, Hanawa-Suetsugu K, Mori Y, Hirabayashi Y, Hirose K, et al.
iScience (2023) 266: 106826. 122 002 ICC; tested species: mouse

α-Synuclein induced cholesterol lowering increases tonic and reduces depolarization-evoked synaptic vesicle recycling and glutamate release.
Lazarevic V, Yang Y, Paslawski W, Svenningsson P
NPJ Parkinson's disease (2022) 81: 71. 122 002 ICC; tested species: mouse

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 122 002 ICC, IHC; tested species: mouse,rat

Complexin facilitates exocytosis and synchronizes vesicle release in two secretory model systems.
Lin MY, Rohan JG, Cai H, Reim K, Ko CP, Chow RH
The Journal of physiology (2013) 59110: 2463-73. 122 002 IHC

Cellular distribution and subcellular localization of molecular components of vesicular transmitter release in horizontal cells of rabbit retina.
Hirano AA, Brandstätter JH, Brecha NC
The Journal of comparative neurology (2005) 4881: 70-81. 122 002 IHC; tested species: rabbit

Cat. No.: 122 002

Amount: 200 µl

Price: $355.00

ICC

IHC

IHC-P

Background

Complexins are enriched in neurons where they colocalize with syntaxin 1 and SNAP 25. In addition, complexin 2, also referred to as synaphin 1, is expressed ubiquitously at low levels. Complexins bind weakly to syntaxin 1 alone and not at all to synaptobrevin and SNAP 25, but strongly to the SNAP receptor-core complex composed of these three molecules. They compete with α-SNAP for binding to the core complex but not with other interacting molecules, suggesting that complexins regulate the sequential interactions of α-SNAP and synaptotagmins with the SNAP receptor during exocytosis.
In retinal ribbon synapses complexin 3 and complexin 4 functionally replace complexin 1 (synaphin 2) and 2. They have similar biochemical binding properties and are farnesylated at their C-terminus.

1 ) Südhof TC et al., Nature (1995)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) McMahon HT et al., Cell (1995)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Complexin1/2 antibody C-terminus - 122 002 K.O.

References for Complexin1/2 - 122 002