Synaptic Systems

Cat. No.: 359 004

Amount: 100 µl

Price: $365.00

Cat. No. 359 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 187 from mouse CAML (UniProt Id: P49070)
Reactivity	Reacts with: rat (Q6DGG9), mouse (P49070). Other species not tested yet.
Specificity	K.O. validated PubMed: 36055406
Data sheet	359_004.pdf

References for CAML - 359 004

The GET insertase exhibits conformational plasticity and induces membrane thinning.
McDowell MA, Heimes M, Enkavi G, Farkas Á, Saar D, Wild K, Schwappach B, Vattulainen I, Sinning I
Nature communications (2023) 141: 7355. 359 004 WB

ER entry pathway and glycosylation of GPI-anchored proteins are determined by N-terminal signal sequence and C-terminal GPI-attachment sequence.
Hirata T, Yang J, Tomida S, Tokoro Y, Kinoshita T, Fujita M, Kizuka Y
The Journal of biological chemistry (2022) : 102444. 359 004 WB; KO verified; tested species: human

A trap mutant reveals the physiological client spectrum of TRC40.
Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science (2019) 13213: . 359 004 WB; tested species: human

Cat. No.: 359 004

Amount: 100 µl

Price: $365.00

Background

The integral membrane protein CAML (Calcium signal-modulating cyclophilin ligand) is highly conserved throughout species and constitutively expressed in immune cells and various cells of the nervous system. Besides its link to calcium signaling, it has recently been shown that CAML reconstitutes together with WRB the TRC40 receptor at the endoplasmic reticulum, which mediates post-translationally insertion of tail-anchored membrane proteins into the ER.

1 ) Vilardi F et al., PLoS ONE (2014)
2 ) Yamamoto Y et al., Mol. Cell (2012)
3 ) Bram RJ et al., Nature (1994)

Protocols

Standard protocol

Other products for this target

CAML antibody - 359 004 K.O.

References for CAML - 359 004