Synaptic Systems - Calretinin

Cat. No.: 214 104

Amount: 100 µl

Price: $370.00

Cat. No. 214 104	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 200 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Full-length recombinant mouse Calretinin (UniProt Id: Q08331)
Reactivity	Reacts with: rat (P47728), mouse (Q08331), chicken, zebrafish. Other species not tested yet.
Matching control protein/peptide	214-1P
Data sheet	214_104.pdf

References for Calretinin - 214 104

Maturation of type I and type II rat vestibular hair cells in vivo and in vitro.
Borrajo M, Sedano D, Palou A, Giménez-Esbrí V, Barrallo-Gimeno A, Llorens J
Frontiers in cell and developmental biology (2024) 12: 1404894. 214 104 IHC; tested species: rat

The vestibular calyceal junction is dismantled following subchronic streptomycin in rats and sensory epithelium stress in humans.
Maroto AF, Borrajo M, Prades S, Callejo À, Amilibia E, Pérez-Grau M, Roca-Ribas F, Castellanos E, Barrallo-Gimeno A, Llorens J
Archives of toxicology (2023) 977: 1943-1961. 214 104 IHC; tested species: rat

Apical-basal distribution of different subtypes of spiral ganglion neurons in the cochlea and the changes during aging.
Wang M, Lin S, Xie R
PloS one (2023) 1810: e0292676. 214 104 IHC; tested species: mouse

Multisensory gaze stabilization in response to subchronic alteration of vestibular type I hair cells.
Schenberg L, Palou A, Simon F, Bonnard T, Barton CE, Fricker D, Tagliabue M, Llorens J, Beraneck M
eLife (2023) 12: . 214 104 IHC; tested species: mouse

Total Number and Ratio of GABAergic Neuron Types in the Mouse Lateral and Basal Amygdala.
Vereczki VK, Müller K, Krizsán É, Máté Z, Fekete Z, Rovira-Esteban L, Veres JM, Erdélyi F, Hájos N
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) 4121: 4575-4595. 214 104 IHC; tested species: mouse

Contribution of GABAergic interneurons to amyloid-β plaque pathology in an APP knock-in mouse model.
Rice HC, Marcassa G, Chrysidou I, Horré K, Young-Pearse TL, Müller UC, Saito T, Saido TC, Vassar R, de Wit J, De Strooper B, et al.
Molecular neurodegeneration (2020) 151: 3. 214 104 IHC; tested species: mouse

Defining the Vulnerability Window of Anesthesia-Induced Neuroapoptosis in Developing Dentate Gyrus Granule Cells - A Transgenic Approach Utilizing POMC-EGFP Mice.
Wei K, Chen P, Shen FY, Zhang Y, Liu YH, Wang ZR, Loepke AW, Wang YW, Deng M
Neuroscience (2019) : . 214 104 IHC; tested species: mouse

Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice.
Mensching L, Djogo N, Keller C, Rading S, Karsak M
International journal of molecular sciences (2019) 2015: . 214 104 IHC; tested species: mouse

Secretagogin expression in the vertebrate brainstem with focus on the noradrenergic system and implications for Alzheimer's disease.
Zahola P, Hanics J, Pintér A, Máté Z, Gáspárdy A, Hevesi Z, Echevarria D, Adori C, Barde S, Törőcsik B, Erdélyi F, et al.
Brain structure & function (2019) : . 214 104 IHC; tested species: rat

VIP-immunoreactive interneurons within circuits of the mouse basolateral amygdala.
Rhomberg T, Rovira-Esteban L, Vikór A, Paradiso E, Kremser C, Nagy-Pál P, Papp OI, Tasan R, Erdélyi F, Szabó G, Ferraguti F, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 214 104 IHC; tested species: mouse

Non-canonical heterogeneous cellular distribution and co-localization of CaMKIIα and CaMKIIβ in the spinal superficial dorsal horn.
Larsson M
Brain structure & function (2018) 2233: 1437-1457. 214 104 IHC; tested species: rat

Multiple amygdaloid divisions of arcopallium send convergent projections to the nucleus accumbens and neighboring subpallial amygdala regions in the domestic chicken: a selective pathway tracing and reconstruction study.
Hanics J, Teleki G, Alpár A, Székely AD, Csillag A
Brain structure & function (2017) 2221: 301-315. 214 104 IHC

Developmental abnormalities of cortical interneurons precede symptoms onset in a mouse model of Rett syndrome.
Tomassy GS, Morello N, Calcagno E, Giustetto M
Journal of neurochemistry (2014) 1311: 115-27. 214 104 IHC; tested species: mouse

Dentate gyrus network dysfunctions precede the symptomatic phase in a genetic mouse model of seizures.
Toader O, Forte N, Orlando M, Ferrea E, Raimondi A, Baldelli P, Benfenati F, Medrihan L
Frontiers in cellular neuroscience (2013) 7: 138. 214 104 IHC; tested species: mouse

Cell age-specific vulnerability of neurons to anesthetic toxicity.
Hofacer RD, Deng M, Ward CG, Joseph B, Hughes EA, Jiang C, Danzer SC, Loepke AW
Annals of neurology (2013) 736: 695-704. 214 104 IHC

Synaptic Organization of VGLUT3 Expressing Low-Threshold Mechanosensitive C Fiber Terminals in the Rodent Spinal Cord.
Larsson M, Broman J
eNeuro () 61: . 214 104 IHC; tested species: rat

Loss of Mecp2 Causes Atypical Synaptic and Molecular Plasticity of Parvalbumin-Expressing Interneurons Reflecting Rett Syndrome-Like Sensorimotor Defects.
Morello N, Schina R, Pilotto F, Phillips M, Melani R, Plicato O, Pizzorusso T, Pozzo-Miller L, Giustetto M
eNeuro () 55: . 214 104 IHC; tested species: mouse

Cat. No.: 214 104

Amount: 100 µl

Price: $370.00

Synaptic Organization of VGLUT3 Expressing Low-Threshold Mechanosensitive C Fiber Terminals in the Rodent Spinal Cord.
Larsson M, Broman J
eNeuro () 61: . 214 104 IHC; tested species: rat

ICC

IHC

IHC-P

Background

Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: Calretinin, also referred to as calbindin D29k, calbindin 2, CALB 2, CAL 2, and CAB 29, and calbindin D28k. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium.

1 ) Klejbor I et al., J. Physiol. Pharmacol. (2006)
2 ) Haworth R et al., Exp. Toxicol. Pathol. (2006)
3 ) Schmidt H et al., J. Physiol. (Lond.) (2003)
4 ) Barski JJ et al., J. Neurosci. (2003)
5 ) Schwaller B et al., Cerebellum (2002)
6 ) Faucheux C et al., Biochem. J. (1998)
7 ) Lee YS et al., Proc. Natl. Acad. Sci. U.S.A. (1987)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Calretinin antibody - 214 104

References for Calretinin - 214 104