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Ca2+ channel P/Q-type alpha-1A antibody - 152 203 K.O.

Rabbit polyclonal purified antibody
Cat. No.: 152 203
Amount: 50 µg
Price: $400.00
Cat. No. 152 203 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: not tested yet
ExM: 1 : 100 (see remarks) gallery  
DNA-PAINT: yes (see remarks)
EM: yes gallery  
Immunogen Recombinant protein corresponding to AA 1921 to 2212 from rat Ca2+ channel P/Q-type α-1A (Cav2.1) (UniProt Id: P54282)
Reactivity Reacts with: rat (P54282), mouse (P97445).
Other species not tested yet.
Specificity K.O. validated PubMed: 27537483
Remarks

WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. Ca2+ channel P/Q-type alpha-1A aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
ExM: This antibody has been successfully used for the magnified analysis of the proteome (MAP) expansion microscopy method (MAP; Ku et al. 2016. Nature Biotechnology 34:973-981)
DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).

Data sheet 152_203.pdf

References for Ca2+ channel P/Q-type alpha-1A - 152 203

The intracellular C-terminus confers compartment-specific targeting of voltage-gated Ca2+ channels.
Chin M, Kaeser PS
bioRxiv : the preprint server for biology (2023) : . 152 203 WB, ICC; KO verified; tested species: mouse
Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht MT, Krause E, Maritzen T, Schmitz D, Haucke V
Neuron (2021) 1092: 299-313.e9. 152 203 WB; tested species: mouse
PRRT2 modulates presynaptic Ca2+ influx by interacting with P/Q-type channels.
Ferrante D, Sterlini B, Prestigio C, Marte A, Corradi A, Onofri F, Tortarolo G, Vicidomini G, Petretto A, Muià J, Thalhammer A, et al.
Cell reports (2021) 3511: 109248. 152 203 WB, ICC; tested species: mouse
Receptor protein tyrosine phosphatase delta is not essential for synapse maintenance or transmission at hippocampal synapses.
Han KA, Lee HY, Lim D, Shin J, Yoon TH, Liu X, Um JW, Choi SY, Ko J
Molecular brain (2020) 131: 94. 152 203 WB; tested species: mouse
Auxiliary α2δ1 and α2δ3 Subunits of Calcium Channels Drive Excitatory and Inhibitory Neuronal Network Development.
Bikbaev A, Ciuraszkiewicz-Wojciech A, Heck J, Klatt O, Freund R, Mitlöhner J, Enrile Lacalle S, Sun M, Repetto D, Frischknecht R, Ablinger C, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4025: 4824-4841. 152 203 WB; tested species: mouse
α-Neurexins Together with α2δ-1 Auxiliary Subunits Regulate Ca2+ Influx through Cav2.1 Channels.
Brockhaus J, Schreitmüller M, Repetto D, Klatt O, Reissner C, Elmslie K, Heine M, Missler M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) 3838: 8277-8294. 152 203 WB, ICC; tested species: mouse
Structural and functional reorganization of inhibitory synapses by activity-dependent cleavage of neuroligin-2.
Xu N, Cao R, Chen SY, Gou XZ, Wang B, Luo HM, Gao F, Tang AH
Proceedings of the National Academy of Sciences of the United States of America (2024) 12118: e2314541121. 152 203 ICC; tested species: mouse
Liprin-α proteins are master regulators of human presynapse assembly.
Marcó de la Cruz B, Campos J, Molinaro A, Xie X, Jin G, Wei Z, Acuna C, Sterky FH
Nature neuroscience (2024) : . 152 203 ICC; tested species: human
The intracellular C-terminus confers compartment-specific targeting of voltage-gated Ca2+ channels.
Chin M, Kaeser PS
bioRxiv : the preprint server for biology (2023) : . 152 203 WB, ICC; KO verified; tested species: mouse
Molecular definition of distinct active zone protein machineries for Ca2+ channel clustering and synaptic vesicle priming.
Emperador-Melero J, Andersen JW, Metzbower SR, Levy AD, Dharmasri PA, de Nola G, Blanpied TA, Kaeser PS
bioRxiv : the preprint server for biology (2023) : . 152 203 ICC, DNA-PAINT; tested species: mouse
Rebuilding essential active zone functions within a synapse.
Tan C, Wang SSH, de Nola G, Kaeser PS
Neuron (2022) 1109: 1498-1515.e8. 152 203 ICC; tested species: mouse
The lipid transporter ORP2 regulates synaptic neurotransmitter release via two distinct mechanisms.
Weber-Boyvat M, Kroll J, Trimbuch T, Olkkonen VM, Rosenmund C
Cell reports (2022) 4113: 111882. 152 203 ICC; tested species: mouse
PRRT2 modulates presynaptic Ca2+ influx by interacting with P/Q-type channels.
Ferrante D, Sterlini B, Prestigio C, Marte A, Corradi A, Onofri F, Tortarolo G, Vicidomini G, Petretto A, Muià J, Thalhammer A, et al.
Cell reports (2021) 3511: 109248. 152 203 WB, ICC; tested species: mouse
PKC-phosphorylation of Liprin-α3 triggers phase separation and controls presynaptic active zone structure.
Emperador-Melero J, Wong MY, Wang SSH, de Nola G, Nyitrai H, Kirchhausen T, Kaeser PS
Nature communications (2021) 121: 3057. 152 203 ICC; tested species: mouse
Intact synapse structure and function after combined knockout of PTPδ, PTPσ, and LAR.
Emperador-Melero J, de Nola G, Kaeser PS
eLife (2021) 10: . 152 203 ICC; tested species: mouse
Synapse and Active Zone Assembly in the Absence of Presynaptic Ca2+ Channels and Ca2+ Entry.
Held RG, Liu C, Ma K, Ramsey AM, Tarr TB, De Nola G, Wang SSH, Wang J, van den Maagdenberg AMJM, Schneider T, Sun J, et al.
Neuron (2020) 1074: 667-683.e9. 152 203 ICC; KO verified; tested species: mouse
Transient Confinement of CaV2.1 Ca2+-Channel Splice Variants Shapes Synaptic Short-Term Plasticity.
Heck J, Parutto P, Ciuraszkiewicz A, Bikbaev A, Freund R, Mitlöhner J, Alonso M, Fejtova A, Holcman D, Heine M
Neuron (2019) : . 152 203 ICC; tested species: human,rat
α-Neurexins Together with α2δ-1 Auxiliary Subunits Regulate Ca2+ Influx through Cav2.1 Channels.
Brockhaus J, Schreitmüller M, Repetto D, Klatt O, Reissner C, Elmslie K, Heine M, Missler M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) 3838: 8277-8294. 152 203 WB, ICC; tested species: mouse
Bicistronic CACNA1A Gene Expression in Neurons Derived from Spinocerebellar Ataxia Type 6 Patient-Induced Pluripotent Stem Cells.
Bavassano C, Eigentler A, Stanika R, Obermair GJ, Boesch S, Dechant G, Nat R
Stem cells and development (2017) 2622: 1612-1625. 152 203 ICC; tested species: human
Alternative Splicing of P/Q-Type Ca2+ Channels Shapes Presynaptic Plasticity.
Thalhammer A, Contestabile A, Ermolyuk YS, Ng T, Volynski KE, Soong TW, Goda Y, Cingolani LA
Cell reports (2017) 202: 333-343. 152 203 ICC; tested species: rat
Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking.
Wang SSH, Held RG, Wong MY, Liu C, Karakhanyan A, Kaeser PS
Neuron (2016) 914: 777-791. 152 203 ICC; KO verified
BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons.
Shinoda Y, Ahmed S, Ramachandran B, Bharat V, Brockelt D, Altas B, Dean C
Frontiers in synaptic neuroscience (2014) 6: 27. 152 203 ICC; tested species: rat
Differential neuronal targeting of a new and two known calcium channel β4 subunit splice variants correlates with their regulation of gene expression.
Etemad S, Obermair GJ, Bindreither D, Benedetti A, Stanika R, Di Biase V, Burtscher V, Koschak A, Kofler R, Geley S, Wille A, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 344: 1446-61. 152 203 ICC; tested species: mouse
Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission.
Spangler SA, Schmitz SK, Kevenaar JT, de Graaff E, de Wit H, Demmers J, Toonen RF, Hoogenraad CC
The Journal of cell biology (2013) 2016: 915-28. 152 203 ICC; tested species: rat
Organization of Purkinje cell development by neuronal MEGF11 in cerebellar granule cells.
Jun S, Kim M, Park H, Hwang E, Yamamoto Y, Tanaka-Yamamoto K
Cell reports (2023) 429: 113137. 152 203 IHC; tested species: mouse
Plastin 3 rescues cell surface translocation and activation of TrkB in spinal muscular atrophy.
Hennlein L, Ghanawi H, Gerstner F, Palominos García E, Yildirim E, Saal-Bauernschubert L, Moradi M, Deng C, Klein T, Appenzeller S, Sauer M, et al.
The Journal of cell biology (2023) 2223: . 152 203 IHC; tested species: mouse
Deletion of the P/Q-type calcium channel from serotonergic neurons drives male aggression in mice.
Bohne P, Volkmann A, Schwarz MK, Mark MD
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) : . 152 203 IHC; tested species: mouse
Neurexins cluster Ca2+ channels within the presynaptic active zone.
Luo F, Sclip A, Jiang M, Südhof TC
The EMBO journal (2020) 397: e103208. 152 203 IHC; tested species: mouse
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 152 203 IHC; tested species: rat
Super-resolution imaging reveals the nanoscale organization of metabotropic glutamate receptors at presynaptic active zones.
Siddig S, Aufmkolk S, Doose S, Jobin ML, Werner C, Sauer M, Calebiro D
Science advances (2020) 616: eaay7193. 152 203 IHC; tested species: mouse
Deletion of the Ca2+ Channel Subunit α2δ3 Differentially Affects Cav2.1 and Cav2.2 Currents in Cultured Spiral Ganglion Neurons Before and After the Onset of Hearing.
Stephani F, Scheuer V, Eckrich T, Blum K, Wang W, Obermair GJ, Engel J
Frontiers in cellular neuroscience (2019) 13: 278. 152 203 IHC; tested species: mouse
Interaction of Axonal Chondrolectin with Collagen XIXa1 Is Necessary for Precise Neuromuscular Junction Formation.
Oprişoreanu AM, Smith HL, Arya S, Webster R, Zhong Z, Wehner D, Cardozo MJ, Becker T, Talbot K, Becker CG
Cell reports (2019) 295: 1082-1098.e10. 152 203 IHC; tested species: mouse
RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses.
Brockmann MM, Maglione M, Willmes CG, Stumpf A, Bouazza BA, Velasquez LM, Grauel MK, Beed P, Lehmann M, Gimber N, Schmoranzer J, et al.
eLife (2019) 8: . 152 203 IHC; tested species: mouse
Multiple roles of integrin-α3 at the neuromuscular junction.
Ross JA, Webster RG, Lechertier T, Reynolds LE, Turmaine M, Bencze M, Jamshidi Y, Cetin H, Muntoni F, Beeson D, Hodilvala-Dilke K, et al.
Journal of cell science (2017) 13010: 1772-1784. 152 203 IHC; tested species: mouse
RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses.
Grauel MK, Maglione M, Reddy-Alla S, Willmes CG, Brockmann MM, Trimbuch T, Rosenmund T, Pangalos M, Vardar G, Stumpf A, Walter AM, et al.
Proceedings of the National Academy of Sciences of the United States of America (2016) 11341: 11615-11620. 152 203 IHC; tested species: mouse
Isolated P/Q Calcium Channel Deletion in Layer VI Corticothalamic Neurons Generates Absence Epilepsy.
Bomben VC, Aiba I, Qian J, Mark MD, Herlitze S, Noebels JL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 362: 405-18. 152 203 IHC; tested species: mouse
Dual-color STED microscopy reveals a sandwich structure of Bassoon and Piccolo in active zones of adult and aged mice.
Nishimune H, Badawi Y, Mori S, Shigemoto K
Scientific reports (2016) 6: 27935. 152 203 IHC; KO verified; tested species: mouse
RIM-BP2 regulates Ca2+ channel abundance and neurotransmitter release at hippocampal mossy fiber terminals.
Miyano R, Sakamoto H, Hirose K, Sakaba T
eLife (2024) 12: . 152 203 IHC-Fr; tested species: mouse
Developmental refinement of the active zone nanotopography and axon wiring at the somatosensory thalamus.
Midorikawa M, Sakamoto H, Nakamura Y, Hirose K, Miyata M
Cell reports (2024) 4310: 114770. 152 203 IHC-Fr; tested species: mouse
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 152 203 DNA-PAINT; tested species: rat
Molecular definition of distinct active zone protein machineries for Ca2+ channel clustering and synaptic vesicle priming.
Emperador-Melero J, Andersen JW, Metzbower SR, Levy AD, Dharmasri PA, de Nola G, Blanpied TA, Kaeser PS
bioRxiv : the preprint server for biology (2023) : . 152 203 ICC, DNA-PAINT; tested species: mouse
Distinct Nanoscale Calcium Channel and Synaptic Vesicle Topographies Contribute to the Diversity of Synaptic Function.
Rebola N, Reva M, Kirizs T, Szoboszlay M, Lőrincz A, Moneron G, Nusser Z, DiGregorio DA
Neuron (2019) 1044: 693-710.e9. 152 203 EM; KO verified; tested species: mouse
Target Cell Type-Dependent Differences in Ca2+ Channel Function Underlie Distinct Release Probabilities at Hippocampal Glutamatergic Terminals.
Éltes T, Kirizs T, Nusser Z, Holderith N
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 377: 1910-1924. 152 203 EM; tested species: rat
Inhibitory and excitatory axon terminals share a common nano-architecture of their Cav2.1 (P/Q-type) Ca(2+) channels.
Althof D, Baehrens D, Watanabe M, Suzuki N, Fakler B, Kulik Á
Frontiers in cellular neuroscience (2015) 9: 315. 152 203 EM
Tonic endocannabinoid-mediated modulation of GABA release is independent of the CB1 content of axon terminals.
Lenkey N, Kirizs T, Holderith N, Máté Z, Szabó G, Vizi ES, Hájos N, Nusser Z
Nature communications (2015) 6: 6557. 152 203 EM
Release probability of hippocampal glutamatergic terminals scales with the size of the active zone.
Holderith N, Lorincz A, Katona G, Rózsa B, Kulik A, Watanabe M, Nusser Z
Nature neuroscience (2012) 157: 988-97. 152 203 EM
Cat. No.: 152 203
Amount: 50 µg
Price: $400.00
The intracellular C-terminus confers compartment-specific targeting of voltage-gated Ca2+ channels.
Chin M, Kaeser PS
bioRxiv : the preprint server for biology (2023) : . 152 203 WB, ICC; KO verified; tested species: mouse
Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht MT, Krause E, Maritzen T, Schmitz D, Haucke V
Neuron (2021) 1092: 299-313.e9. 152 203 WB; tested species: mouse
PRRT2 modulates presynaptic Ca2+ influx by interacting with P/Q-type channels.
Ferrante D, Sterlini B, Prestigio C, Marte A, Corradi A, Onofri F, Tortarolo G, Vicidomini G, Petretto A, Muià J, Thalhammer A, et al.
Cell reports (2021) 3511: 109248. 152 203 WB, ICC; tested species: mouse
Receptor protein tyrosine phosphatase delta is not essential for synapse maintenance or transmission at hippocampal synapses.
Han KA, Lee HY, Lim D, Shin J, Yoon TH, Liu X, Um JW, Choi SY, Ko J
Molecular brain (2020) 131: 94. 152 203 WB; tested species: mouse
Auxiliary α2δ1 and α2δ3 Subunits of Calcium Channels Drive Excitatory and Inhibitory Neuronal Network Development.
Bikbaev A, Ciuraszkiewicz-Wojciech A, Heck J, Klatt O, Freund R, Mitlöhner J, Enrile Lacalle S, Sun M, Repetto D, Frischknecht R, Ablinger C, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4025: 4824-4841. 152 203 WB; tested species: mouse
α-Neurexins Together with α2δ-1 Auxiliary Subunits Regulate Ca2+ Influx through Cav2.1 Channels.
Brockhaus J, Schreitmüller M, Repetto D, Klatt O, Reissner C, Elmslie K, Heine M, Missler M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) 3838: 8277-8294. 152 203 WB, ICC; tested species: mouse
Structural and functional reorganization of inhibitory synapses by activity-dependent cleavage of neuroligin-2.
Xu N, Cao R, Chen SY, Gou XZ, Wang B, Luo HM, Gao F, Tang AH
Proceedings of the National Academy of Sciences of the United States of America (2024) 12118: e2314541121. 152 203 ICC; tested species: mouse
Liprin-α proteins are master regulators of human presynapse assembly.
Marcó de la Cruz B, Campos J, Molinaro A, Xie X, Jin G, Wei Z, Acuna C, Sterky FH
Nature neuroscience (2024) : . 152 203 ICC; tested species: human
The intracellular C-terminus confers compartment-specific targeting of voltage-gated Ca2+ channels.
Chin M, Kaeser PS
bioRxiv : the preprint server for biology (2023) : . 152 203 WB, ICC; KO verified; tested species: mouse
Molecular definition of distinct active zone protein machineries for Ca2+ channel clustering and synaptic vesicle priming.
Emperador-Melero J, Andersen JW, Metzbower SR, Levy AD, Dharmasri PA, de Nola G, Blanpied TA, Kaeser PS
bioRxiv : the preprint server for biology (2023) : . 152 203 ICC, DNA-PAINT; tested species: mouse
Rebuilding essential active zone functions within a synapse.
Tan C, Wang SSH, de Nola G, Kaeser PS
Neuron (2022) 1109: 1498-1515.e8. 152 203 ICC; tested species: mouse
The lipid transporter ORP2 regulates synaptic neurotransmitter release via two distinct mechanisms.
Weber-Boyvat M, Kroll J, Trimbuch T, Olkkonen VM, Rosenmund C
Cell reports (2022) 4113: 111882. 152 203 ICC; tested species: mouse
PRRT2 modulates presynaptic Ca2+ influx by interacting with P/Q-type channels.
Ferrante D, Sterlini B, Prestigio C, Marte A, Corradi A, Onofri F, Tortarolo G, Vicidomini G, Petretto A, Muià J, Thalhammer A, et al.
Cell reports (2021) 3511: 109248. 152 203 WB, ICC; tested species: mouse
PKC-phosphorylation of Liprin-α3 triggers phase separation and controls presynaptic active zone structure.
Emperador-Melero J, Wong MY, Wang SSH, de Nola G, Nyitrai H, Kirchhausen T, Kaeser PS
Nature communications (2021) 121: 3057. 152 203 ICC; tested species: mouse
Intact synapse structure and function after combined knockout of PTPδ, PTPσ, and LAR.
Emperador-Melero J, de Nola G, Kaeser PS
eLife (2021) 10: . 152 203 ICC; tested species: mouse
Synapse and Active Zone Assembly in the Absence of Presynaptic Ca2+ Channels and Ca2+ Entry.
Held RG, Liu C, Ma K, Ramsey AM, Tarr TB, De Nola G, Wang SSH, Wang J, van den Maagdenberg AMJM, Schneider T, Sun J, et al.
Neuron (2020) 1074: 667-683.e9. 152 203 ICC; KO verified; tested species: mouse
Transient Confinement of CaV2.1 Ca2+-Channel Splice Variants Shapes Synaptic Short-Term Plasticity.
Heck J, Parutto P, Ciuraszkiewicz A, Bikbaev A, Freund R, Mitlöhner J, Alonso M, Fejtova A, Holcman D, Heine M
Neuron (2019) : . 152 203 ICC; tested species: human,rat
α-Neurexins Together with α2δ-1 Auxiliary Subunits Regulate Ca2+ Influx through Cav2.1 Channels.
Brockhaus J, Schreitmüller M, Repetto D, Klatt O, Reissner C, Elmslie K, Heine M, Missler M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) 3838: 8277-8294. 152 203 WB, ICC; tested species: mouse
Bicistronic CACNA1A Gene Expression in Neurons Derived from Spinocerebellar Ataxia Type 6 Patient-Induced Pluripotent Stem Cells.
Bavassano C, Eigentler A, Stanika R, Obermair GJ, Boesch S, Dechant G, Nat R
Stem cells and development (2017) 2622: 1612-1625. 152 203 ICC; tested species: human
Alternative Splicing of P/Q-Type Ca2+ Channels Shapes Presynaptic Plasticity.
Thalhammer A, Contestabile A, Ermolyuk YS, Ng T, Volynski KE, Soong TW, Goda Y, Cingolani LA
Cell reports (2017) 202: 333-343. 152 203 ICC; tested species: rat
Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking.
Wang SSH, Held RG, Wong MY, Liu C, Karakhanyan A, Kaeser PS
Neuron (2016) 914: 777-791. 152 203 ICC; KO verified
BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons.
Shinoda Y, Ahmed S, Ramachandran B, Bharat V, Brockelt D, Altas B, Dean C
Frontiers in synaptic neuroscience (2014) 6: 27. 152 203 ICC; tested species: rat
Differential neuronal targeting of a new and two known calcium channel β4 subunit splice variants correlates with their regulation of gene expression.
Etemad S, Obermair GJ, Bindreither D, Benedetti A, Stanika R, Di Biase V, Burtscher V, Koschak A, Kofler R, Geley S, Wille A, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 344: 1446-61. 152 203 ICC; tested species: mouse
Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission.
Spangler SA, Schmitz SK, Kevenaar JT, de Graaff E, de Wit H, Demmers J, Toonen RF, Hoogenraad CC
The Journal of cell biology (2013) 2016: 915-28. 152 203 ICC; tested species: rat
Organization of Purkinje cell development by neuronal MEGF11 in cerebellar granule cells.
Jun S, Kim M, Park H, Hwang E, Yamamoto Y, Tanaka-Yamamoto K
Cell reports (2023) 429: 113137. 152 203 IHC; tested species: mouse
Plastin 3 rescues cell surface translocation and activation of TrkB in spinal muscular atrophy.
Hennlein L, Ghanawi H, Gerstner F, Palominos García E, Yildirim E, Saal-Bauernschubert L, Moradi M, Deng C, Klein T, Appenzeller S, Sauer M, et al.
The Journal of cell biology (2023) 2223: . 152 203 IHC; tested species: mouse
Deletion of the P/Q-type calcium channel from serotonergic neurons drives male aggression in mice.
Bohne P, Volkmann A, Schwarz MK, Mark MD
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) : . 152 203 IHC; tested species: mouse
Neurexins cluster Ca2+ channels within the presynaptic active zone.
Luo F, Sclip A, Jiang M, Südhof TC
The EMBO journal (2020) 397: e103208. 152 203 IHC; tested species: mouse
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 152 203 IHC; tested species: rat
Super-resolution imaging reveals the nanoscale organization of metabotropic glutamate receptors at presynaptic active zones.
Siddig S, Aufmkolk S, Doose S, Jobin ML, Werner C, Sauer M, Calebiro D
Science advances (2020) 616: eaay7193. 152 203 IHC; tested species: mouse
Deletion of the Ca2+ Channel Subunit α2δ3 Differentially Affects Cav2.1 and Cav2.2 Currents in Cultured Spiral Ganglion Neurons Before and After the Onset of Hearing.
Stephani F, Scheuer V, Eckrich T, Blum K, Wang W, Obermair GJ, Engel J
Frontiers in cellular neuroscience (2019) 13: 278. 152 203 IHC; tested species: mouse
Interaction of Axonal Chondrolectin with Collagen XIXa1 Is Necessary for Precise Neuromuscular Junction Formation.
Oprişoreanu AM, Smith HL, Arya S, Webster R, Zhong Z, Wehner D, Cardozo MJ, Becker T, Talbot K, Becker CG
Cell reports (2019) 295: 1082-1098.e10. 152 203 IHC; tested species: mouse
RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses.
Brockmann MM, Maglione M, Willmes CG, Stumpf A, Bouazza BA, Velasquez LM, Grauel MK, Beed P, Lehmann M, Gimber N, Schmoranzer J, et al.
eLife (2019) 8: . 152 203 IHC; tested species: mouse
Multiple roles of integrin-α3 at the neuromuscular junction.
Ross JA, Webster RG, Lechertier T, Reynolds LE, Turmaine M, Bencze M, Jamshidi Y, Cetin H, Muntoni F, Beeson D, Hodilvala-Dilke K, et al.
Journal of cell science (2017) 13010: 1772-1784. 152 203 IHC; tested species: mouse
RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses.
Grauel MK, Maglione M, Reddy-Alla S, Willmes CG, Brockmann MM, Trimbuch T, Rosenmund T, Pangalos M, Vardar G, Stumpf A, Walter AM, et al.
Proceedings of the National Academy of Sciences of the United States of America (2016) 11341: 11615-11620. 152 203 IHC; tested species: mouse
Isolated P/Q Calcium Channel Deletion in Layer VI Corticothalamic Neurons Generates Absence Epilepsy.
Bomben VC, Aiba I, Qian J, Mark MD, Herlitze S, Noebels JL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 362: 405-18. 152 203 IHC; tested species: mouse
Dual-color STED microscopy reveals a sandwich structure of Bassoon and Piccolo in active zones of adult and aged mice.
Nishimune H, Badawi Y, Mori S, Shigemoto K
Scientific reports (2016) 6: 27935. 152 203 IHC; KO verified; tested species: mouse
RIM-BP2 regulates Ca2+ channel abundance and neurotransmitter release at hippocampal mossy fiber terminals.
Miyano R, Sakamoto H, Hirose K, Sakaba T
eLife (2024) 12: . 152 203 IHC-Fr; tested species: mouse
Developmental refinement of the active zone nanotopography and axon wiring at the somatosensory thalamus.
Midorikawa M, Sakamoto H, Nakamura Y, Hirose K, Miyata M
Cell reports (2024) 4310: 114770. 152 203 IHC-Fr; tested species: mouse
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 152 203 DNA-PAINT; tested species: rat
Molecular definition of distinct active zone protein machineries for Ca2+ channel clustering and synaptic vesicle priming.
Emperador-Melero J, Andersen JW, Metzbower SR, Levy AD, Dharmasri PA, de Nola G, Blanpied TA, Kaeser PS
bioRxiv : the preprint server for biology (2023) : . 152 203 ICC, DNA-PAINT; tested species: mouse
Distinct Nanoscale Calcium Channel and Synaptic Vesicle Topographies Contribute to the Diversity of Synaptic Function.
Rebola N, Reva M, Kirizs T, Szoboszlay M, Lőrincz A, Moneron G, Nusser Z, DiGregorio DA
Neuron (2019) 1044: 693-710.e9. 152 203 EM; KO verified; tested species: mouse
Target Cell Type-Dependent Differences in Ca2+ Channel Function Underlie Distinct Release Probabilities at Hippocampal Glutamatergic Terminals.
Éltes T, Kirizs T, Nusser Z, Holderith N
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 377: 1910-1924. 152 203 EM; tested species: rat
Inhibitory and excitatory axon terminals share a common nano-architecture of their Cav2.1 (P/Q-type) Ca(2+) channels.
Althof D, Baehrens D, Watanabe M, Suzuki N, Fakler B, Kulik Á
Frontiers in cellular neuroscience (2015) 9: 315. 152 203 EM
Tonic endocannabinoid-mediated modulation of GABA release is independent of the CB1 content of axon terminals.
Lenkey N, Kirizs T, Holderith N, Máté Z, Szabó G, Vizi ES, Hájos N, Nusser Z
Nature communications (2015) 6: 6557. 152 203 EM
Release probability of hippocampal glutamatergic terminals scales with the size of the active zone.
Holderith N, Lorincz A, Katona G, Rózsa B, Kulik A, Watanabe M, Nusser Z
Nature neuroscience (2012) 157: 988-97. 152 203 EM
Background

Voltage gated calcium channels (VGCCs), also referred to as voltage sensitive calcium channels (VSCCs), are present in most excitable cells. They mediate the influx of Ca2+ ions into the cell and trigger the release of neurotransmitters or hormons but are also involved in other calcium dependent processes like metabolism, cell proliferation and cell death.
VGCCs are composed of four subunits (α-1, α-2, β and δ) in a 1:1:1:1 ratio. The α-1A isoform occurs in VGCCs of the P/Q-type while isoform α-1B is found in the N-type. Both belong to the high voltage activated group (hva).