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Home > Products > 386 004

Ankyrin G antibody - 386 004 K.D.

AnkyrinG is a membrane-cytoskeleton linker
Guinea pig polyclonal antiserum
Cat. No.: 386 004
Amount: 100 µl
Price: $370.00
Cat. No. 386 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of mouse Ankyrin G. (UniProt Id: G5E8K5-1)
Reactivity Reacts with: rat (O70511-1), mouse (G5E8K5-1).
Other species not tested yet.
Specificity Specific for Ankyrin G; detects all described splice variants. K.D. validated PubMed: 31727776
Data sheet 386_004.pdf

References for Ankyrin G - 386 004

Postsynaptic β1 spectrin maintains Na+ channels at the neuromuscular junction.
Sert O, Ding X, Zhang C, Mi R, Hoke A, Rasband MN
The Journal of physiology (2024) 6026: 1127-1145. 386 004 WB; tested species: mouse
Membrane mechanics dictate axonal pearls-on-a-string morphology and function.
Griswold JM, Bonilla-Quintana M, Pepper R, Lee CT, Raychaudhuri S, Ma S, Gan Q, Syed S, Zhu C, Bell M, Suga M, et al.
Nature neuroscience (2024) : . 386 004 ICC; tested species: mouse
Transcriptomic alterations in APP/PS1 mice astrocytes lead to early postnatal axon initial segment structural changes.
Benitez MJ, Retana D, Ordoñez-Gutiérrez L, Colmena I, Goméz MJ, Álvarez R, Ciorraga M, Dopazo A, Wandosell F, Garrido JJ
Cellular and molecular life sciences : CMLS (2024) 811: 444. 386 004 ICC; tested species: mouse
Immunoproximity biotinylation reveals the axon initial segment proteome.
Zhang W, Fu Y, Peng L, Ogawa Y, Ding X, Rasband A, Zhou X, Shelly M, Rasband MN, Zou P
Nature communications (2023) 141: 8201. 386 004 ICC, IHC; tested species: rat
Disruption of tubulin-alpha4a polyglutamylation prevents aggregation of hyper-phosphorylated tau and microglia activation in mice.
Hausrat TJ, Janiesch PC, Breiden P, Lutz D, Hoffmeister-Ullerich S, Hermans-Borgmeyer I, Failla AV, Kneussel M
Nature communications (2022) 131: 4192. 386 004 ICC; tested species: mouse
The amyloid precursor protein is a conserved Wnt receptor.
Liu T, Zhang T, Nicolas M, Boussicault L, Rice H, Soldano A, Claeys A, Petrova I, Fradkin L, De Strooper B, Potier MC, et al.
eLife (2021) 10: . 386 004 ICC; tested species: mouse
NuMA1 promotes axon initial segment assembly through inhibition of endocytosis.
Torii T, Ogawa Y, Liu CH, Ho TS, Hamdan H, Wang CC, Oses-Prieto JA, Burlingame AL, Rasband MN
The Journal of cell biology (2020) 2192: . 386 004 ICC; KD verified; tested species: mouse
TRIM46 organizes microtubule fasciculation in the axon initial segment.
Harterink M, Vocking K, Pan X, Soriano Jerez EM, Slenders L, Fréal A, Tas RP, van de Wetering WJ, Timmer K, Motshagen J, van Beuningen SFB, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 386 004 ICC; tested species: rat
The awakening of dormant neuronal precursors in the adult and aged brain.
Benedetti B, Reisinger M, Hochwartner M, Gabriele G, Jakubecova D, Benedetti A, Bonfanti L, Couillard-Despres S
Aging cell (2023) : e13974. 386 004 IHC; tested species: mouse
Immunoproximity biotinylation reveals the axon initial segment proteome.
Zhang W, Fu Y, Peng L, Ogawa Y, Ding X, Rasband A, Zhou X, Shelly M, Rasband MN, Zou P
Nature communications (2023) 141: 8201. 386 004 ICC, IHC; tested species: rat
Chronic partial TrkB activation reduces seizures and mortality in a mouse model of Dravet syndrome.
Gu F, Parada I, Yang T, Longo FM, Prince DA
Proceedings of the National Academy of Sciences of the United States of America (2022) 1197: . 386 004 IHC; tested species: mouse
Aging-Associated Changes in Cognition, Expression and Epigenetic Regulation of Chondroitin 6-Sulfotransferase Chst3.
Baidoe-Ansah D, Sakib S, Jia S, Mirzapourdelavar H, Strackeljan L, Fischer A, Aleshin S, Kaushik R, Dityatev A
Cells (2022) 1113: . 386 004 IHC; tested species: mouse
Morphological properties of the axon initial segment-like process of AII amacrine cells in the rat retina.
Liu JH, Singh JB, Veruki ML, Hartveit E
The Journal of comparative neurology (2021) 52916: 3593-3620. 386 004 IHC; tested species: rat
Granule Cell Ensembles in Mouse Dentate Gyrus Rapidly Upregulate the Plasticity-Related Protein Synaptopodin after Exploration Behavior.
Paul MH, Choi M, Schlaudraff J, Deller T, Del Turco D
Cerebral cortex (New York, N.Y. : 1991) (2019) : . 386 004 IHC; tested species: mouse
An ankyrin G-binding motif mediates TRAAK periodic localization at axon initial segments of hippocampal pyramidal neurons.
Luque-Fernández V, Vanspauwen SK, Landra-Willm A, Arvedsen E, Besquent M, Sandoz G, Rasmussen HB
Proceedings of the National Academy of Sciences of the United States of America (2024) 12131: e2310120121. 386 004 IHC-Fr; tested species: rat
Cat. No.: 386 004
Amount: 100 µl
Price: $370.00
Postsynaptic β1 spectrin maintains Na+ channels at the neuromuscular junction.
Sert O, Ding X, Zhang C, Mi R, Hoke A, Rasband MN
The Journal of physiology (2024) 6026: 1127-1145. 386 004 WB; tested species: mouse
Membrane mechanics dictate axonal pearls-on-a-string morphology and function.
Griswold JM, Bonilla-Quintana M, Pepper R, Lee CT, Raychaudhuri S, Ma S, Gan Q, Syed S, Zhu C, Bell M, Suga M, et al.
Nature neuroscience (2024) : . 386 004 ICC; tested species: mouse
Transcriptomic alterations in APP/PS1 mice astrocytes lead to early postnatal axon initial segment structural changes.
Benitez MJ, Retana D, Ordoñez-Gutiérrez L, Colmena I, Goméz MJ, Álvarez R, Ciorraga M, Dopazo A, Wandosell F, Garrido JJ
Cellular and molecular life sciences : CMLS (2024) 811: 444. 386 004 ICC; tested species: mouse
Immunoproximity biotinylation reveals the axon initial segment proteome.
Zhang W, Fu Y, Peng L, Ogawa Y, Ding X, Rasband A, Zhou X, Shelly M, Rasband MN, Zou P
Nature communications (2023) 141: 8201. 386 004 ICC, IHC; tested species: rat
Disruption of tubulin-alpha4a polyglutamylation prevents aggregation of hyper-phosphorylated tau and microglia activation in mice.
Hausrat TJ, Janiesch PC, Breiden P, Lutz D, Hoffmeister-Ullerich S, Hermans-Borgmeyer I, Failla AV, Kneussel M
Nature communications (2022) 131: 4192. 386 004 ICC; tested species: mouse
The amyloid precursor protein is a conserved Wnt receptor.
Liu T, Zhang T, Nicolas M, Boussicault L, Rice H, Soldano A, Claeys A, Petrova I, Fradkin L, De Strooper B, Potier MC, et al.
eLife (2021) 10: . 386 004 ICC; tested species: mouse
NuMA1 promotes axon initial segment assembly through inhibition of endocytosis.
Torii T, Ogawa Y, Liu CH, Ho TS, Hamdan H, Wang CC, Oses-Prieto JA, Burlingame AL, Rasband MN
The Journal of cell biology (2020) 2192: . 386 004 ICC; KD verified; tested species: mouse
TRIM46 organizes microtubule fasciculation in the axon initial segment.
Harterink M, Vocking K, Pan X, Soriano Jerez EM, Slenders L, Fréal A, Tas RP, van de Wetering WJ, Timmer K, Motshagen J, van Beuningen SFB, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 386 004 ICC; tested species: rat
The awakening of dormant neuronal precursors in the adult and aged brain.
Benedetti B, Reisinger M, Hochwartner M, Gabriele G, Jakubecova D, Benedetti A, Bonfanti L, Couillard-Despres S
Aging cell (2023) : e13974. 386 004 IHC; tested species: mouse
Immunoproximity biotinylation reveals the axon initial segment proteome.
Zhang W, Fu Y, Peng L, Ogawa Y, Ding X, Rasband A, Zhou X, Shelly M, Rasband MN, Zou P
Nature communications (2023) 141: 8201. 386 004 ICC, IHC; tested species: rat
Chronic partial TrkB activation reduces seizures and mortality in a mouse model of Dravet syndrome.
Gu F, Parada I, Yang T, Longo FM, Prince DA
Proceedings of the National Academy of Sciences of the United States of America (2022) 1197: . 386 004 IHC; tested species: mouse
Aging-Associated Changes in Cognition, Expression and Epigenetic Regulation of Chondroitin 6-Sulfotransferase Chst3.
Baidoe-Ansah D, Sakib S, Jia S, Mirzapourdelavar H, Strackeljan L, Fischer A, Aleshin S, Kaushik R, Dityatev A
Cells (2022) 1113: . 386 004 IHC; tested species: mouse
Morphological properties of the axon initial segment-like process of AII amacrine cells in the rat retina.
Liu JH, Singh JB, Veruki ML, Hartveit E
The Journal of comparative neurology (2021) 52916: 3593-3620. 386 004 IHC; tested species: rat
Granule Cell Ensembles in Mouse Dentate Gyrus Rapidly Upregulate the Plasticity-Related Protein Synaptopodin after Exploration Behavior.
Paul MH, Choi M, Schlaudraff J, Deller T, Del Turco D
Cerebral cortex (New York, N.Y. : 1991) (2019) : . 386 004 IHC; tested species: mouse
An ankyrin G-binding motif mediates TRAAK periodic localization at axon initial segments of hippocampal pyramidal neurons.
Luque-Fernández V, Vanspauwen SK, Landra-Willm A, Arvedsen E, Besquent M, Sandoz G, Rasmussen HB
Proceedings of the National Academy of Sciences of the United States of America (2024) 12131: e2310120121. 386 004 IHC-Fr; tested species: rat
WB
ICC
IHC
IHC
IHC-P
IHC-P
Background
Ankyrin G is a membrane-cytoskeleton linker. It may participate in the targeting and clustering of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments (AIS).
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