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Home > Products > 386 003

Ankyrin G antibody - 386 003

AnkyrinG is a membrane-cytoskeleton linker
Rabbit polyclonal purified antibody
Cat. No.: 386 003
Amount: 50 µg
Price: $380.00
Cat. No. 386 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500      gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of mouse Ankyrin G. (UniProt Id: G5E8K5-1)
Reactivity Reacts with: rat (O70511-1), mouse (G5E8K5-1), human (Q12955).
Other species not tested yet.
Specificity Specific for Ankyrin G, detects all described splice variants.
Data sheet 386_003.pdf

References for Ankyrin G - 386 003

Impaired OTUD7A-dependent Ankyrin regulation mediates neuronal dysfunction in mouse and human models of the 15q13.3 microdeletion syndrome.
Unda BK, Chalil L, Yoon S, Kilpatrick S, Irwin C, Xing S, Murtaza N, Cheng A, Brown C, Afonso A, McCready E, et al.
Molecular psychiatry (2023) 284: 1747-1769. 386 003 WB, ICC; tested species: human,mouse
Neurofilament Levels in Dendritic Spines Associate with Synaptic Status.
Gürth CM, do Rego Barros Fernandes Lima MA, Macarrón Palacios V, Cereceda Delgado AR, Hubrich J, D'Este E
Cells (2023) 126: . 386 003 ICC; tested species: rat
Spectrin-beta 2 facilitates the selective accumulation of GABAA receptors at somatodendritic synapses.
Smalley JL, Cho N, Ng SFJ, Choi C, Lemons AHS, Chaudry S, Bope CE, Dengler JS, Zhang C, Rasband MN, Davies PA, et al.
Communications biology (2023) 61: 11. 386 003 ICC; tested species: mouse
Impaired OTUD7A-dependent Ankyrin regulation mediates neuronal dysfunction in mouse and human models of the 15q13.3 microdeletion syndrome.
Unda BK, Chalil L, Yoon S, Kilpatrick S, Irwin C, Xing S, Murtaza N, Cheng A, Brown C, Afonso A, McCready E, et al.
Molecular psychiatry (2023) 284: 1747-1769. 386 003 WB, ICC; tested species: human,mouse
Endocytosis in the axon initial segment maintains neuronal polarity.
Eichel K, Uenaka T, Belapurkar V, Lu R, Cheng S, Pak JS, Taylor CA, Südhof TC, Malenka R, Wernig M, Özkan E, et al.
Nature (2022) : . 386 003 ICC; tested species: mouse
The core PCP protein Prickle2 regulates axon number and AIS maturation by binding to AnkG and modulating microtubule bundling.
Dorrego-Rivas A, Ezan J, Moreau MM, Poirault-Chassac S, Aubailly N, De Neve J, Blanchard C, Castets F, Fréal A, Battefeld A, Sans N, et al.
Science advances (2022) 836: eabo6333. 386 003 ICC; tested species: rat
Neuregulins 1, 2, and 3 Promote Early Neurite Outgrowth in ErbB4-Expressing Cortical GABAergic Interneurons.
Rahman-Enyart A, Lai C, Prieto AL
Molecular neurobiology (2020) : . 386 003 ICC; tested species: rat
Phosphorylation on Ser 359 of the α2 subunit in GABA type A receptors down-regulates their density at inhibitory synapses.
Nakamura Y, Morrow DH, Nathanson AJ, Henley JM, Wilkinson KA, Moss SJ
The Journal of biological chemistry (2020) : . 386 003 ICC; tested species: rat
Loss of Neurofascin-186 Disrupts Alignment of AnkyrinG Relative to Its Binding Partners in the Axon Initial Segment.
Alpizar SA, Baker AL, Gulledge AT, Hoppa MB
Frontiers in cellular neuroscience (2019) 13: 1. 386 003 ICC; tested species: rat
Development of myelination and axon diameter for fast and precise action potential conductance.
Nabel AL, Teich L, Wohlfrom H, Alexandrova O, Heß M, Pecka M, Grothe B
Glia (2024) : . 386 003 IHC
Motor learning changes the axon initial segment of the spinal motoneuron.
Wang Y, Chen Y, Chen L, Herron BJ, Chen XY, Wolpaw JR
The Journal of physiology (2024) 6029: 2107-2126. 386 003 IHC; tested species: rat
Kv7/KCNQ potassium channels in cortical hyperexcitability and juvenile seizure-related death in Ank2-mutant mice.
Oh H, Lee S, Oh Y, Kim S, Kim YS, Yang Y, Choi W, Yoo YE, Cho H, Lee S, Yang E, et al.
Nature communications (2023) 141: 3547. 386 003 IHC; tested species: mouse
Development of microglia in fetal and postnatal neocortex of the pig, the European wild boar (Sus scrofa).
Sobierajski E, Lauer G, Aktas M, Beemelmans C, Beemelmans C, Meyer G, Wahle P
The Journal of comparative neurology (2021) : . 386 003 IHC; tested species: pig
Deletion of class II ARFs in mice causes tremor by the Nav1.6 loss in cerebellar Purkinje cell axon initial segments.
Hosoi N, Shibasaki K, Hosono M, Konno A, Shinoda Y, Kiyonari H, Inoue K, Muramatsu SI, Ishizaki Y, Hirai H, Furuichi T, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 386 003 IHC; tested species: mouse
Cat. No.: 386 003
Amount: 50 µg
Price: $380.00
Impaired OTUD7A-dependent Ankyrin regulation mediates neuronal dysfunction in mouse and human models of the 15q13.3 microdeletion syndrome.
Unda BK, Chalil L, Yoon S, Kilpatrick S, Irwin C, Xing S, Murtaza N, Cheng A, Brown C, Afonso A, McCready E, et al.
Molecular psychiatry (2023) 284: 1747-1769. 386 003 WB, ICC; tested species: human,mouse
Neurofilament Levels in Dendritic Spines Associate with Synaptic Status.
Gürth CM, do Rego Barros Fernandes Lima MA, Macarrón Palacios V, Cereceda Delgado AR, Hubrich J, D'Este E
Cells (2023) 126: . 386 003 ICC; tested species: rat
Spectrin-beta 2 facilitates the selective accumulation of GABAA receptors at somatodendritic synapses.
Smalley JL, Cho N, Ng SFJ, Choi C, Lemons AHS, Chaudry S, Bope CE, Dengler JS, Zhang C, Rasband MN, Davies PA, et al.
Communications biology (2023) 61: 11. 386 003 ICC; tested species: mouse
Impaired OTUD7A-dependent Ankyrin regulation mediates neuronal dysfunction in mouse and human models of the 15q13.3 microdeletion syndrome.
Unda BK, Chalil L, Yoon S, Kilpatrick S, Irwin C, Xing S, Murtaza N, Cheng A, Brown C, Afonso A, McCready E, et al.
Molecular psychiatry (2023) 284: 1747-1769. 386 003 WB, ICC; tested species: human,mouse
Endocytosis in the axon initial segment maintains neuronal polarity.
Eichel K, Uenaka T, Belapurkar V, Lu R, Cheng S, Pak JS, Taylor CA, Südhof TC, Malenka R, Wernig M, Özkan E, et al.
Nature (2022) : . 386 003 ICC; tested species: mouse
The core PCP protein Prickle2 regulates axon number and AIS maturation by binding to AnkG and modulating microtubule bundling.
Dorrego-Rivas A, Ezan J, Moreau MM, Poirault-Chassac S, Aubailly N, De Neve J, Blanchard C, Castets F, Fréal A, Battefeld A, Sans N, et al.
Science advances (2022) 836: eabo6333. 386 003 ICC; tested species: rat
Neuregulins 1, 2, and 3 Promote Early Neurite Outgrowth in ErbB4-Expressing Cortical GABAergic Interneurons.
Rahman-Enyart A, Lai C, Prieto AL
Molecular neurobiology (2020) : . 386 003 ICC; tested species: rat
Phosphorylation on Ser 359 of the α2 subunit in GABA type A receptors down-regulates their density at inhibitory synapses.
Nakamura Y, Morrow DH, Nathanson AJ, Henley JM, Wilkinson KA, Moss SJ
The Journal of biological chemistry (2020) : . 386 003 ICC; tested species: rat
Loss of Neurofascin-186 Disrupts Alignment of AnkyrinG Relative to Its Binding Partners in the Axon Initial Segment.
Alpizar SA, Baker AL, Gulledge AT, Hoppa MB
Frontiers in cellular neuroscience (2019) 13: 1. 386 003 ICC; tested species: rat
Development of myelination and axon diameter for fast and precise action potential conductance.
Nabel AL, Teich L, Wohlfrom H, Alexandrova O, Heß M, Pecka M, Grothe B
Glia (2024) : . 386 003 IHC
Motor learning changes the axon initial segment of the spinal motoneuron.
Wang Y, Chen Y, Chen L, Herron BJ, Chen XY, Wolpaw JR
The Journal of physiology (2024) 6029: 2107-2126. 386 003 IHC; tested species: rat
Kv7/KCNQ potassium channels in cortical hyperexcitability and juvenile seizure-related death in Ank2-mutant mice.
Oh H, Lee S, Oh Y, Kim S, Kim YS, Yang Y, Choi W, Yoo YE, Cho H, Lee S, Yang E, et al.
Nature communications (2023) 141: 3547. 386 003 IHC; tested species: mouse
Development of microglia in fetal and postnatal neocortex of the pig, the European wild boar (Sus scrofa).
Sobierajski E, Lauer G, Aktas M, Beemelmans C, Beemelmans C, Meyer G, Wahle P
The Journal of comparative neurology (2021) : . 386 003 IHC; tested species: pig
Deletion of class II ARFs in mice causes tremor by the Nav1.6 loss in cerebellar Purkinje cell axon initial segments.
Hosoi N, Shibasaki K, Hosono M, Konno A, Shinoda Y, Kiyonari H, Inoue K, Muramatsu SI, Ishizaki Y, Hirai H, Furuichi T, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 386 003 IHC; tested species: mouse
WB
ICC
IHC
IHC
IHC-P
Background
Ankyrin G is a membrane-cytoskeleton linker. It may participate in the targeting and clustering of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments (AIS).
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