Synaptic Systems

Cat. No.: 218 202

Amount: 200 µl

Price: $355.00

WB

IHC-P

Background

Amyloid deposits, also called plaques, of Alzheimer's patients consist of several protein components like the amyloid beta-peptides (Abeta, Aβ) 1-40/42/43 and additional C- and N-terminally modified fragments of Abeta as for instance Abeta pE3 and Abeta pE11.
An additional Abeta variant, Abeta38, is more soluble compared to other Abeta species and is not found in plaques of sporadic Alzheimer´s cases. However, it is detected in the blood-vessel walls of a subset of patients with severe cerebral amyloid angiopathy. It especially accumulates in brains of patients carrying mutations in the Abeta coding region.
Cleavage of amyloid precursor protein APP by β- and γ- secretases results in the generation of the Aβ (βA4)peptide, whereas α-secretase cleaves within the Aβ sequence and prevents the formation of Abeta from APP.

1 ) Marcello A et al., J Neural Transm (Vienna) (2009)
2 ) Baril L et al., Neurosci. Lett. (2004)
3 ) Bayer TA et al., Proc. Natl. Acad. Sci. U.S.A. (2003)
4 ) Hsiao K et al., Science (1996)
5 ) Wood SJ et al., J. Mol. Biol. (1996)
6 ) Kuo YM et al., J. Biol. Chem. (1996)

Protocols

WB

IHC-P

Other products for this target

Cat. No. 218 202	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet (see remarks) IHC-P: 1 : 100 up to 1 : 500 (see remarks) gallery ELISA: (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Immunogen	Synthetic peptide corresponding to AA 33 to 40 from human Abeta40 (UniProt Id: P05067)
Reactivity	Reacts with: human (P05067), rat (P08592), mouse (P12023). Other species not tested yet.
Specificity	Specific for Abeta 40, no cross reactivity to Abeta 38 and 42.
Remarks	WB: Detects purified Abeta 40. Complex samples like brain extracts still have to be tested. Boil membrane after blotting for 3min. IHC: Antigen retrieval with formic acid is required. IHC-P: Antigen retrieval with formic acid is required. ELISA: suitable as capture and detector antibody for sandwich-ELISA.
Data sheet	218_202.pdf

Abeta40 antibody - 218 202