Synaptic Systems - alpha/beta SNAP

Cat. No.: 112 011

Amount: 100 µg

Price: $420.00

Cat. No. 112 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery IP: yes (see remarks) ICC: not recommended (denaturation of the antigen during fixation) IHC: not tested yet IHC-P: not tested yet
Clone	77.1
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 295 from rat α SNAP (UniProt Id: P54921)
Reactivity	Reacts with: human (P54920, P60880), rat (P54921, P60881), mouse (Q9DB05, P28663), zebrafish. Other species not tested yet.
Specificity	Specific for α- and β SNAP, does not cross-react to γ SNAP.
Remarks	IP: The antibody does not immunoprecipitate the 20 S SNARE-complex.
Data sheet	112_011.pdf

CSPα knockout causes neurodegeneration by impairing SNAP-25 function.
Sharma M, Burré J, Bronk P, Zhang Y, Xu W, Südhof TC
The EMBO journal (2012) 314: 829-41. 112 011 WB; tested species: mouse

Sperm from hyh mice carrying a point mutation in alphaSNAP have a defect in acrosome reaction.
Bátiz LF, De Blas GA, Michaut MA, Ramírez AR, Rodríguez F, Ratto MH, Oliver C, Tomes CN, Rodríguez EM, Mayorga LS
PloS one (2009) 43: e4963. 112 011 WB; tested species: mouse

alpha-SNAP and NSF are required in a priming step during the human sperm acrosome reaction.
Tomes CN, De Blas GA, Michaut MA, Farré EV, Cherhitin O, Visconti PE, Mayorga LS
Molecular human reproduction (2005) 111: 43-51. 112 011 WB; tested species: human

The N-ethylmaleimide-sensitive fusion protein and alpha-SNAP induce a conformational change in syntaxin.
Hanson PI, Otto H, Barton N, Jahn R
The Journal of biological chemistry (1995) 27028: 16955-61. 112 011 WB

Cat. No.: 112 011

Amount: 100 µg

Price: $420.00

Background

Protocols

alpha/beta SNAP antibody - 112 011