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alpha/beta SNAP antibody - 112 011

alpha/beta SNAP are peripheral membrane proteins required for membrane traffic
Mouse monoclonal purified IgG
Cat. No.: 112 011
Amount: 100 µg
Price: $415.00
Cat. No. 112 011 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery  
IP: yes (see remarks)
ICC: not recommended (denaturation of the antigen during fixation)
IHC: not tested yet
IHC-P: not tested yet
Clone 77.1
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 295 from rat α SNAP (UniProt Id: P54921)
Reactivity Reacts with: human (P54920, P60880), rat (P54921, P60881), mouse (Q9DB05, P28663), zebrafish.
Other species not tested yet.
Specificity Specific for α- and β SNAP, does not cross-react to γ SNAP.
Remarks

IP: The antibody does not immunoprecipitate the 20 S SNARE-complex.

Data sheet 112_011.pdf

References for alpha/beta SNAP - 112 011

CSPα knockout causes neurodegeneration by impairing SNAP-25 function.
Sharma M, Burré J, Bronk P, Zhang Y, Xu W, Südhof TC
The EMBO journal (2012) 314: 829-41. 112 011 WB; tested species: mouse
Sperm from hyh mice carrying a point mutation in alphaSNAP have a defect in acrosome reaction.
Bátiz LF, De Blas GA, Michaut MA, Ramírez AR, Rodríguez F, Ratto MH, Oliver C, Tomes CN, Rodríguez EM, Mayorga LS
PloS one (2009) 43: e4963. 112 011 WB; tested species: mouse
alpha-SNAP and NSF are required in a priming step during the human sperm acrosome reaction.
Tomes CN, De Blas GA, Michaut MA, Farré EV, Cherhitin O, Visconti PE, Mayorga LS
Molecular human reproduction (2005) 111: 43-51. 112 011 WB; tested species: human
The N-ethylmaleimide-sensitive fusion protein and alpha-SNAP induce a conformational change in syntaxin.
Hanson PI, Otto H, Barton N, Jahn R
The Journal of biological chemistry (1995) 27028: 16955-61. 112 011 WB
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 112 011
Cat. No.: 112 011
Amount: 100 µg
Price: $415.00
CSPα knockout causes neurodegeneration by impairing SNAP-25 function.
Sharma M, Burré J, Bronk P, Zhang Y, Xu W, Südhof TC
The EMBO journal (2012) 314: 829-41. 112 011 WB; tested species: mouse
Sperm from hyh mice carrying a point mutation in alphaSNAP have a defect in acrosome reaction.
Bátiz LF, De Blas GA, Michaut MA, Ramírez AR, Rodríguez F, Ratto MH, Oliver C, Tomes CN, Rodríguez EM, Mayorga LS
PloS one (2009) 43: e4963. 112 011 WB; tested species: mouse
alpha-SNAP and NSF are required in a priming step during the human sperm acrosome reaction.
Tomes CN, De Blas GA, Michaut MA, Farré EV, Cherhitin O, Visconti PE, Mayorga LS
Molecular human reproduction (2005) 111: 43-51. 112 011 WB; tested species: human
The N-ethylmaleimide-sensitive fusion protein and alpha-SNAP induce a conformational change in syntaxin.
Hanson PI, Otto H, Barton N, Jahn R
The Journal of biological chemistry (1995) 27028: 16955-61. 112 011 WB
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 112 011
Background

The proteins α/β-SNAP are two related soluble and highly conserved proteins that bind to the fusion complex (SNARE complex), thus allowing the N-ethylmaleimide sensitive fusion protein NSF to bind to the complex. γ-SNAP binds directly to NSF and Gaf-1/Rip11, a protein of the Rab11 interacting family. In contrast to α/β-SNAP it does not interact directly with SNARE proteins and is not required for ER-Golgi transport. SNAP-proteins are abundantly expressed in all tissues. They are partially soluble, partially membrane-bound.