Cat. No.: N3583
Amount: 100 µg
Price:
$515.00
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This product was developed by |
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FluoTags are camelid single domain antibodies consisting only of one antigen binding site of a Alpaka heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
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| Cat. No. N3583 |
100 µg purified recombinant IgG, lyophilized. Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications |
IHC: yes |
| Clone | W25 |
| Subtype | IgG1 |
| Immunogen | recombinant S1-Spike protein from SARS-CoV-2 (UniProt Id: P0DTC2) |
| Specificity | RBD of S1 protein from SARS-CoV-2 |
| Remarks |
This antibody is a chimeric construct consisting of the anti- single-domain S1 SARS-CoV-2 antibody W25 genetically fused to rabbit IgG1 Fc domain. It can be used in combination with standard anti-rabbit secondary reagents binding the IgG Fc-domain. |
| Data sheet | n3583.pdf |
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This anti-S1 SARS-CoV-2 nanobody was developed by the team of Alejandro Rojas-Fernandez at the Institute of Medicine from the Universidad Austral de Chile. NanoTag acquired the exclusive license to produce this nanobody and make it available or research and development purposes. The FluoTag-Q anti-S1 SARS-CoV-2 is based on the W25 clone, which has a sub-nanomolar affinity for the SARS-CoV-2 S1 protein receptor binding domain (RBD) and can neutralize infections as it efficiently competes with ACE-2 receptor binding.
In FluoTag-X two fluorophore molecules are site-specifically coupled to each FluoTag molecule which ensures bright signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is below 4 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.