Cat. No.: N3105-DBCO
Amount: 250 µg
Price:
$770.00
|
This product was developed by |
 |
Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
|
|
| Cat. No. N3105-DBCO |
250 µg purified antibody, lyophilized from PBS, fluorescence-labeled with DBCO.
Dibenzocyclooctyne (DBCO, ADIBO) is one of the most reactive cycloalkynes for strain promoted alkyne azide cycloaddition (spAAC), a copper-free click chemistry reaction. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Applications |
|
| Label | DBCO |
| Clone | 1E8 |
| Immunogen | recombinant mEos (UniProt Id: Q5S6Z9) |
| Specificity | Recognizes mEOS and mEOS derivatives |
|
|
mEOS, is a bright monomeric green photoswitchable fluorescent protein derived from Lobophyllia hemprichii.
Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
In FluoTag®-X2 two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets two fluorophores to the protein of interest, which ensures up to two-fold („2X“)-brighter signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.