Cat. No.: N3105-250ug
Amount: 250 µg
Price:
$355.00
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This product was developed by |
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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
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| Cat. No. N3105-250ug |
250 µg purified antibody, lyophilized from PBS. For reconstitution add 250 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
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| Clone | 1E8 |
| Immunogen | recombinant mEos (UniProt Id: Q5S6Z9) |
| Specificity | Recognizes mEOS and mEOS derivatives |
| Remarks |
This single domain antibody carries a C-terminal cystein available for conjugation. |
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mEOS, is a bright monomeric green photoswitchable fluorescent protein derived from Lobophyllia hemprichii.
Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
In FluoTag®-X2 two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets two fluorophores to the protein of interest, which ensures up to two-fold („2X“)-brighter signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.