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anti-Rabbit IgG sdAb - N2405-HRP

A monovalent single domain secondary antibody specific for rabbit IgG

 

This product was developed by   

NanoTag-Biotechnologies

Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody
Cat. No.: N2405-HRP
Amount: 200 µl
Price: $515.00
Cat. No. N2405-HRP 200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with HRP.

Horseradish peroxidase - usually abbreviated as HRP, is one of the most commonly used reporter enzymes in biochemistry. It can be used with a huge panel of chromogenic and fluorescent substrates.

Albumin was added for stabilization. For reconstitution add 200 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: yes
Label HRP
Clone 10E10
Reactivity Reacts with: rabbit.
No signal: chicken, mouse, Guinea pig, donkey, rat, goat, pig, horse, cow, human.
Other species not tested yet.
Specificity Rabbit IgG, does not cross-react with mouse, guinea pig or chicken immunoglobulins

References for anti-Rabbit IgG sdAb - N2405-HRP

METTL3 Promotes Osteogenic Differentiation of Human Periodontal Ligament Stem Cells through IGF2BP1-Mediated Regulation of Runx2 Stability.
Sun X, Meng X, Piao Y, Dong S, Dong Q
International journal of medical sciences (2024) 214: 664-673. N2405-HRP WB; tested species: human
Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives.
Kilisch M, Gere-Becker M, Wüstefeld L, Bonnas C, Crauel A, Mechmershausen M, Martens H, Götzke H, Opazo F, Frey S
International journal of molecular sciences (2023) 248: . N2405-HRP WB; tested species: mouse
Cat. No.: N2405-HRP
Amount: 200 µl
Price: $515.00
METTL3 Promotes Osteogenic Differentiation of Human Periodontal Ligament Stem Cells through IGF2BP1-Mediated Regulation of Runx2 Stability.
Sun X, Meng X, Piao Y, Dong S, Dong Q
International journal of medical sciences (2024) 214: 664-673. N2405-HRP WB; tested species: human
Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives.
Kilisch M, Gere-Becker M, Wüstefeld L, Bonnas C, Crauel A, Mechmershausen M, Martens H, Götzke H, Opazo F, Frey S
International journal of molecular sciences (2023) 248: . N2405-HRP WB; tested species: mouse
Background

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.

 

FluoTag®-X anti-Rabbit is a species-specific FluoTag® directed against the IgG from Rabbit. In FluoTag-X2, two fluorophore molecules are site-specifically coupled to one individual FluoTag molecule. The reagent therefore targets four fluorophores to your primary Rabbit antibody. FluoTag-X4 is a blend of two nanobodies carrying two fluorophores each. Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. Since FluoTags are recombinantly expressed, there is no batch-to-batch variation.