Cat. No.: N2405-DBCO
Amount: 250 µg
Price:
$770.00
|
This product was developed by |
 |
Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
|
|
| Cat. No. N2405-DBCO |
250 µg purified antibody, lyophilized from PBS, fluorescence-labeled with DBCO.
Dibenzocyclooctyne (DBCO, ADIBO) is one of the most reactive cycloalkynes for strain promoted alkyne azide cycloaddition (spAAC), a copper-free click chemistry reaction. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Applications |
ICC: yes IHC: yes FACS: yes |
| Label | DBCO |
| Clone | 10E10 |
| Reactivity |
Reacts with: rabbit. No signal: chicken, mouse, Guinea pig, donkey, rat, goat, pig, horse, cow, human. Other species not tested yet. |
| Specificity | Rabbit IgG, does not cross-react with mouse, guinea pig or chicken immunoglobulins |
|
|
Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
FluoTag®-X anti-Rabbit is a species-specific FluoTag® directed against the IgG from Rabbit. In FluoTag-X2, two fluorophore molecules are site-specifically coupled to one individual FluoTag molecule. The reagent therefore targets four fluorophores to your primary Rabbit antibody. FluoTag-X4 is a blend of two nanobodies carrying two fluorophores each. Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. Since FluoTags are recombinantly expressed, there is no batch-to-batch variation.