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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
Cat. No. N2405-250ug | 250 µg purified antibody, lyophilized from PBS. For reconstitution add 250 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. |
Applications |
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Clone | 10E10 |
Reactivity |
Reacts with: rabbit. No signal: chicken, mouse, Guinea pig, donkey, rat, goat, pig, horse, cow, human. Other species not tested yet. |
Specificity | Rabbit IgG, does not cross-react with mouse, guinea pig or chicken immunoglobulins |
Remarks |
This single domain antibody carries a C-terminal cystein available for conjugation. |
Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
FluoTag®-X anti-Rabbit is a species-specific FluoTag® directed against the IgG from Rabbit. In FluoTag-X2, two fluorophore molecules are site-specifically coupled to one individual FluoTag molecule. The reagent therefore targets four fluorophores to your primary Rabbit antibody. FluoTag-X4 is a blend of two nanobodies carrying two fluorophores each. Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. Since FluoTags are recombinantly expressed, there is no batch-to-batch variation.