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Home > Products > N2404-At488-S

anti-Rabbit IgG sdAb - FluoTag-X4 - N2404-At488-S

Secondary single domain antibody specific for rabbit IgG

 

This product was developed by   

 NanoTag-Biotechnologies

Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N2404-At488-S
Amount: 500 µl
Price: $250.00
Cat. No. N2404-At488-S 500 µl purified antibody, lyophilized from PBS, fluorescence-labeled with ATTO® 488.

For many of the fluorescence labeled antibodies conjugated to ATTO® dyes from ATTO-TEC the established fluorescence detection systems can be used.

ATTO® 488: λex 500 nm / λem 520 nm
ATTO® 550: λex 554 nm / λem 576 nm
ATTO® 565: λex 564 nm / λem 590 nm
ATTO® 594: λex 603 nm / λem 626 nm
ATTO® 647N: λex 646 nm / λem 664 nm
 

ATTO®488, ATTO®565, ATTO®647N and ATTO®594 dyes are suitable for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy.
ATTO®488, ATTO®565 and ATTO®594 are also recommended for PALM and dSTORM high resolution microscopy.

This product or portions thereof is manufactured under license from ATTO-TEC GmbH.
ATTO is a trademarks of ATTO-TEC GmbH, Siegen/Germany.
Purchase of reagents related to the AbberiorStar technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from ATTO-TEC GmbH.

 Albumin was added for stabilization. For reconstitution add 500 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Storage Up to 3 months: –20 °C
Up to 12 months: –80 °C or below
Protect from light!
Applications
 
ICC: 1 : 500 gallery  
IHC: 1 : 500
Label ATTO 488, two fluorophores coupled to two FluoTags each
Clone 8C10/10E10
Subtype single domain
Reactivity Reacts with: rabbit.
No signal: chicken, mouse, Guinea pig, donkey, rat, goat, pig, horse, cow, human.
Other species not tested yet.

Cat. No.: N2404-At488-S
Amount: 500 µl
Price: $250.00
ICC
Background

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.

 

FluoTag®-X anti-Rabbit is a species-specific FluoTag® directed against the IgG from Rabbit. In FluoTag-X2, two fluorophore molecules are site-specifically coupled to one individual FluoTag molecule. The reagent therefore targets four fluorophores to your primary Rabbit antibody. FluoTag-X4 is a blend of two nanobodies carrying two fluorophores each. Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. Since FluoTags are recombinantly expressed, there is no batch-to-batch variation.
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