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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
|Cat. No. N2002-At488-S||
500 µl purified antibody, lyophilized from PBS, fluorescence-labeled with ATTO® 488
For many of the fluorescence labeled antibodies conjugated to ATTO® dyes from
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Up to 3 months: –20 °C
Up to 12 months: –80 °C or below
Protect from light!
Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500
Fluorescence-activated cell sorting (FACS); usually cells are fluorescently labeled with antibodies against cell-surface antigens followed by cell-sorter analysis.', $event)" style="cursor: help;">FACS: yes
|Label||ATTO 488, two fluorophores coupled to one FluoTag|
Reacts with: mouse.
No signal: rabbit, chicken, Guinea pig, donkey, rat, goat, pig, horse, cow, human.
Other species not tested yet.
|Specificity||Recognizes Mouse IgG1 isotype|
Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
FluoTag®-X2 anti-Mouse is a species-specific FluoTag® directed against the IgG1 subtype from Mouse. In FluoTag-X2, two fluorophore molecules are site-specifically coupled to one individual FluoTag molecule. The reagent therefore targets four fluorophores to your primary Mouse antibody. Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. Since our FluoTags are recombinantly expressed, there is no batch-to-batch variation.