Ubiquitin K48 Selector affinity resin - N1810

Ubiquitin (Ub) is a small (8.6 kDa) regulatory protein. By conjugation of one or more ubiquitin molecules (monoubiquination or polyubiquination) almost all eukaryotic can be modified posttranslationally. modification that affects nearly all proteins in eukaryotes.Different ubiquination patterns have different effects on the tagged proteins, e.g. they can be targeted for degradation of the proteasome, their cellular localization or activity can be affected. The Ubiquitin Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads and specifically recognizes the ubiquitin residues for immunoprecipitations. The Ubiquitin pan Selector will purify ubiquitin residues irrespective of its linkage to other subunits or its multimeric state. The Ubiquitin K48 Selector recognizes only polyubiquitin chains that have a K48 linkage, a linkage that often related to degradation of the modified protein via the proteasome. The K63 linkage of ubiquitin may regulate the endocytic trafficking of the modified protein. This linkage is specifically precipitated by the Ubiquitin K63 Selector.

• Immunoprecipitation with Selector Resins