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VGluT 1 sdAb - FluoTag-X2 - N1602-AF647-L

Glutamate transporter in the membrane of synaptic vesicles

 

This product was developed by   

NanoTag-Biotechnologies

FluoTags are camelid single domain antibodies consisting only of one antigen binding site of a Alpaka heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Single Domain

Cat. No.: N1602-AF647-L
Amount: 200 µl
Price: $485.00
Cat. No. N1602-AF647-L

200µl 5µM camelid sdAb in buffered saline, 50% glycerol, 0.09% sodium azide

Storage Up to three months: -20°C
Up to 12 months: -80°C or below
Protect from light!
Applications WB: not recommended
IP: N/A
ICC: 1 : 500
IHC: not tested yet
IHC-P/FFPE: not tested yet
FACS: yes
Label Alexa 647, two fluorophores coupled to one FluoTag
Clone Nb9
Subtype single domain
Immunogen Recombinant protein corresponding to AA 58 to 515 from rat VGLUT1 (UniProt Id: Q62634)
Epitop Epitop: AA 58 to 515 from rat VGLUT1 (UniProt Id: Q62634)
Reactivity Reacts with: rat (Q62634), mouse (Q3TXX4).
Other species not tested yet.
Specificity Specific for VGLUT 1
Documents data sheet

Cat. No.: N1602-AF647-L
Quantity: 200 µl
Price: $485.00
Background

The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.

 

In FluoTag-X two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is below 4 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.

Protocols