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Home > Products > N1302-At488-L

anti-mScarlet-i sdAb - FluoTag-X2 - N1302-At488-L

mScarlet is a bright monomeric red fluorescent protein tag

This product was developed by   

 NanoTag-Biotechnologies

Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N1302-At488-L
Amount: 200 µl
Price: $515.00
Cat. No. N1302-At488-L 200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with ATTO® 488.

For many of the fluorescence labeled antibodies conjugated to ATTO® dyes from ATTO-TEC the established fluorescence detection systems can be used.

ATTO® 488: λex 500 nm / λem 520 nm
ATTO® 550: λex 554 nm / λem 576 nm
ATTO® 565: λex 564 nm / λem 590 nm
ATTO® 594: λex 603 nm / λem 626 nm
ATTO® 647N: λex 646 nm / λem 664 nm
 

ATTO®488, ATTO®565, ATTO®647N and ATTO®594 dyes are suitable for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy.
ATTO®488, ATTO®565 and ATTO®594 are also recommended for PALM and dSTORM high resolution microscopy.

This product or portions thereof is manufactured under license from ATTO-TEC GmbH.
ATTO is a trademarks of ATTO-TEC GmbH, Siegen/Germany.
Purchase of reagents related to the AbberiorStar technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from ATTO-TEC GmbH.

 Albumin was added for stabilization. For reconstitution add 200 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Storage Up to three months: -20°C
Up to 12 months: -80°C or below
Protect from light!
Applications
 
WB: not recommended
IP: N/A
ICC: 1 : 500
IHC-P: not tested yet
FACS: yes
Label ATTO 488, two fluorophores coupled to one FluoTag
Clone 2B12
Subtype single domain
Immunogen Recombinant protein corresponding to AA 1 to 232 from sea anemone mScarlet-i
Specificity Specific for mScarlet-i and most common RFP derivatives

Cat. No.: N1302-At488-L
Amount: 200 µl
Price: $515.00
Background

mScarlet-i is a basic, very bright red fluorescent protein, derived from a synthetic construct (1). It is reported to be a rapidly-maturing, constitutively fluorescent monomer with moderate acid sensitivity. It is a useful acceptor in Foerster resonance energy transfer (FRET) based imaging techniques (2).

 

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.


In FluoTag®-X2 two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets two  fluorophores to the protein of interest, which ensures up to two-fold („2X“)-brighter signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.
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