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Home > Products > N1205-HRP

anti-Mouse IgG kappa light chain sdAb - N1205-HRP

A monovalent single domain secondary antibody specific for mouse IgG kappa LC

 

This product was developed by   

 NanoTag-Biotechnologies

Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody
Cat. No.: N1205-HRP
Amount: 200 µl
Price: $515.00
Cat. No. N1205-HRP 200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with HRP.

Horseradish peroxidase - usually abbreviated as HRP, is one of the most commonly used reporter enzymes in biochemistry. It can be used with a huge panel of chromogenic and fluorescent substrates.
Albumin was added for stabilization. For reconstitution add 200 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: yes

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.
Label HRP
Clone 1A23
Reactivity Reacts with: mouse.
No signal: chicken, rabbit, Guinea pig, donkey, rat, goat, pig, horse, cow, human.
Other species not tested yet.
Specificity Mouse IgG (Kappa light chain), does not cross-react with rabbit, guinea pig or chicken immunoglobulins
Cat. No.: N1205-HRP
Amount: 200 µl
Price: $515.00
Background

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.

 

FluoTag®-X2 anti-Mouse is a species-specific FluoTag® directed against the κ light chain of mouse.
In FluoTag®-X2, two fluorophore molecules are coupled site-specifically to one individual FluoTag® molecule. Therefore, the reagent targets four fluorophores to your primary mouse antibody.
Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. Since our FluoTags are recombinantly expressed, there is no batch-to-batch variation.
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