anti-Mouse IgG kappa light chain sdAb - FluoTag-X2 - N1202-Li800-S

Secondary single domain antibody specific for mouse IgG with a kappa light chain

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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N1202-Li800-S

Amount: 500 µl

Price: $250.00

Cat. No. N1202-Li800-S	500 µl purified antibody, lyophilized from PBS. Albumin was added for stabilization. For reconstitution add 500 µl H₂O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Storage	Up to three months: -20°C Up to 12 months: -80°C or below Protect from light!
Applications	WB: yes ICC: 1 : 500 IHC: 1 : 500 FACS: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Fluorescence-activated cell sorting (FACS); usually cells are fluorescently labeled with antibodies against cell-surface antigens followed by cell-sorter analysis.
Label	Licor 800CW, two fluorophores coupled to one FluoTag
Clone	1A23
Subtype	single domain
Reactivity	Reacts with: mouse. No signal: chicken, rabbit, Guinea pig, donkey, rat, goat, pig, horse, cow, human. Other species not tested yet.
Specificity	Recognizes Mouse IgG (Kappa light chain), does not cross-react with rabbit, Guinea pig or chicken immunoglobulins

Cat. No.: N1202-Li800-S

Amount: 500 µl

Price: $250.00

Background

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.

FluoTag^®-X2 anti-Mouse is a species-specific FluoTag^® directed against the κ light chain of mouse.
In FluoTag^®-X2, two fluorophore molecules are coupled site-specifically to one individual FluoTag^® molecule. Therefore, the reagent targets four fluorophores to your primary mouse antibody.
Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. Since our FluoTags are recombinantly expressed, there is no batch-to-batch variation.

Protocols

ICC

IHC

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