Cat. No.: N0404-AF647-L
Amount: 200 µl
Price:
$515.00
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This product was developed by |
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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
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| Cat. No. N0404-AF647-L |
200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with Alexa 647.
Fluorescence labeled antibodies conjugated to Alexa® dyes are well suited for standard epi-fluorescence setups and confocal microscopy. AZdye® 568: λex 568 nm / λem 665 nm (identical to Alexa® 568) This product or portions thereof is manufactured under license from Life Technologies Corporation. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Storage |
Up to three months: -20°C Up to 12 months: -80°C or below Protect from light! |
| Applications |
IP: N/A ICC: 1 : 500 IHC: not tested yet IHC-P: not tested yet FACS: yes |
| Label | Alexa 647, two fluorophores coupled to two FluoTags each |
| Clone | 2B12-2A1 |
| Subtype | single domain |
| Immunogen | Recombinant protein corresponding to AA 1 to 225 from sea anemone RFP (UniProt Id: Q9U6Y8) |
| Specificity |
Recognizes mRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed, mOrange Does not cross-react with GFP or mTagBFP derivatives. |
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Red fluorescent protein RFP is a common fusion protein used in cell biology to follow the localization and expression of proteins of interest. RFP is known for its rapid maturation and high resistance to photobleaching and therefore well suited for multi color imaging.
Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
In FluoTag®-Q each fluorophore is coupled to exactly one FluoTag, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a coupling efficiency of > 95% guarantees a highly linear relation between the number of target molecules and the intensity of fluorescence. This enables a direct count of the target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
In FluoTag®-X two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets up to four fluorophores (in X4 variants) to the protein of interest, which ensures extra-bright signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.