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This product was developed by |
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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
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Cat. No. N0301-AF647-L |
200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with Alexa 647. Albumin was added for stabilization. For reconstitution add 200 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
Storage |
Up to three months: -20°C Up to 12 months: -80°C or below Protect from light! |
Applications |
IP: N/A ICC: 1 : 500 IHC: not tested yet IHC-P: not tested yet FACS: yes |
Label | Alexa 647, one fluorophore coupled to one FluoTag |
Clone | 1H1 |
Subtype | single domain |
Immunogen | Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212) |
Specificity | Recognizes GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine |
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