Tailor-made Antibodies
and Tools for Life Science

GFP sdAb - FluoTag-Q - N0301-AF647-L

GFP is a widely used green fluorescent protein tag

 

This product was developed by   

NanoTag-Biotechnologies

Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N0301-AF647-L
Amount: 200 µl
Price: $515.00
Cat. No. N0301-AF647-L 200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with Alexa 647.
Albumin was added for stabilization. For reconstitution add 200 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Storage Up to three months: -20°C
Up to 12 months: -80°C or below
Protect from light!
Applications
 
WB: not recommended
IP: N/A
ICC: 1 : 500
IHC: not tested yet
IHC-P: not tested yet
FACS: yes
Label Alexa 647, one fluorophore coupled to one FluoTag
Clone 1H1
Subtype single domain
Immunogen Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212)
Specificity Recognizes GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine

References for GFP sdAb - N0301-AF647-L

CLASP-mediated competitive binding in protein condensates directs microtubule growth.
Jia X, Lin L, Guo S, Zhou L, Jin G, Dong J, Xiao J, Xie X, Li Y, He S, Wei Z, et al.
Nature communications (2024) 151: 6509. N0301-AF647-L ICC; tested species: human

Cat. No.: N0301-AF647-L
Amount: 200 µl
Price: $515.00