Cat. No.: N0301-AF647-L
Amount: 200 µl
Price:
$515.00
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This product was developed by |
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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
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| Cat. No. N0301-AF647-L |
200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with
Alexa 647.
Albumin was added for stabilization. For reconstitution add 200 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Storage |
Up to three months: -20°C Up to 12 months: -80°C or below Protect from light! |
| Applications |
IP: N/A ICC: 1 : 500 IHC: not tested yet IHC-P: not tested yet FACS: yes |
| Label | Alexa 647, one fluorophore coupled to one FluoTag |
| Clone | 1H1 |
| Subtype | single domain |
| Immunogen | Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212) |
| Specificity | Recognizes GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine |
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Green fluorescent protein GFP and its derivates have become very popular and universal tools in cell biology. It is a monomeric and fast maturating protein with high photostability. Due to its sensitivity to pH changes it can be used as a biological pH indicator.
Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
In FluoTag®-Q each fluorophore is coupled to exactly one FluoTag, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a coupling efficiency of > 95% guarantees a highly linear relation between the number of target molecules and the intensity of fluorescence. This enables a direct count of the target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
In FluoTag®-X two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets up to four fluorophores (in X4 variants) to the protein of interest, which ensures extra-bright signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.