Synaptic Systems

Cat. No.: HS-521 003

Amount: 50 µg

Price: $370.00

IHC

IHC-P

Background

Maf (musculoaponeurotic fibrosarcoma) family proteins belong to the basic-leucine zipper family of transcription factors and are divided into large Maf and small Maf proteins depending on their molecular size. Four types of large Maf transcription factors have been identified in mice and humans: MafA, MafB, c-Maf and NRL (1). MafA is a β-cell-specific transcription factor in adult pancreatic islets and regulates glucose-stimulated insulin secretion (2). MafB, on the other hand, is specifically expressed in glucagon-expressing α-cells in the pancreas of adult mice. In the embryonic or neonatal pancreas, immature β-cells first express MafB, whereas MafA is expressed in mature β-cells and is essential for the maintenance of the mature phenotype (3). Several studies have shown, that the expression of MafA is impaired in β-cells of rodents and humans with diabetes (3). In addition to its role in the pancreas, MafA is also involved in the development of neuronal cells. For example, MafA is involved in the development of glutamatergic neurons in the olfactory bulb (4) and of dorsal root ganglia in the spinal cord (5).

1 ) Takahashi S et al., Exp Anim (2021)
2 ) Kataoka K et al., J Biol Chem (2002)
3 ) Nishimura W et al., Int J Mol Sci (2022)
4 ) Ito A et al., Dev Neurobiol (2022)
5 ) Hu J et al., J Neurosci (2012)

Cat. No. HS-521 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet IHC: 1 : 1000 gallery IHC-P: 1 : 2000 up to 1 : 4000 gallery ELISA: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse MafA (UniProt Id: Q8CF90)
Reactivity	Reacts with: mouse (Q8CF90), rat (D3ZNT6). Other species not tested yet.
Data sheet	hs-521_003.pdf

MafA antibody - HS-521 003