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CLEC4F antibody N-terminal - HS-501 103

CLEC4F is a marker for Kupffer cells
Rabbit polyclonal purified antibody
Cat. No.: HS-501 103
Amount: 50 µg
Price: $370.00
Cat. No. HS-501 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 (AP-staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 (see remarks) gallery  
IHC-P: 1 : 2000 gallery  
Immunogen Synthetic peptide corresponding to residues near the amino terminus of mouse CLEC4F (UniProt Id: P70194)
Reactivity Reacts with: mouse (P70194).
Other species not tested yet.
Remarks

IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is recommended for immunohistochemical staining

Data sheet hs-501_103.pdf
Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.
Cat. No.: HS-501 103
Amount: 50 µg
Price: $370.00
Background

The murine CLEC4F is a hallmark of mouse Kupffer cells (KCs) and its expression distinguishes KCs and recruited macrophages (1). CLEC4F is a C-type lectin participating in the antigen presentation of glycolipid antigens, as well as in the recognition and scavenging of desialylated platelets (2). The human CLEC4F gene does not encode a functional receptor due to a mutation in the splice acceptor site of the final exon preventing the appropriate splicing and a missense mutation disrupts the sugar-binding site (3). CLEC4F colocalises with F4/80. Although F4/80 is constitutively expressed in peripheral and resident macrophages, CLEC4F is inducible in the liver and its expression appears when primitive macrophages migrate into the fetal liver. Moreover, CLEC4F and F4/80 cells in the liver increase after infection (4).