Synaptic Systems

Cat. No.: HS-500 003

Amount: 50 µg

Price: $370.00

WB

IHC

IHC-P

Background

Arginase-1 (Arg-1) is a cytosolic protein primarily known for its role in the urea cycle within the liver. It has gained increasing attention in the field of immunology due to its significant influence on immune responses and various disease states. Arg-1 converts arginine to urea and ornithine in the last step of the urea cycle in the liver. Arg-1 is expressed in myeloid cells, including macrophages and neutrophils. Its function in the urea pathway plays a crucial role in regulating the production of nitric oxide (NO), a key signaling molecule involved in various immune processes. Arg-1 competes with inducible nitric oxide synthase (iNOS) for arginine, and its activity can skew the immune response towards a more anti-inflammatory or "M2" phenotype in macrophages by limiting NO production (1). Dysregulation of Arg-1 in microglia has been associated with neurodegenerative diseases, such as Alzheimer's and Parkinson's disease (2). An inherited deficiency of this enzyme leads to argininemia, an autosomal recessive disorder characterized by hyperammonemia (3).

1 ) Cherry JD et al., J Neuroinflammation (2014)
2 ) Wu G et al., Biochem J (1998)
3 ) Sprecher CA et al., Biochemistry (1993)

Other products for this target

Cat. No. HS-500 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: yes (AP-Staining) gallery IP: not tested yet ICC: not tested yet IHC: yes (see remarks) gallery IHC-P: yes gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse Arg1 (UniProt Id: Q61176)
Reactivity	Reacts with: mouse (Q61176). Weaker signal: rat (P07824), human (P05089). Other species not tested yet.
Remarks	IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical staining
Data sheet	hs-500_003.pdf

Arg-1 antibody C-terminal - HS-500 003