|Cat. No. HS-491 017||
100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 200 up to 1 : 1000 gallery
Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-Fr: 1 : 500 (see remarks) gallery
|Subtype||IgG2a (κ light chain)|
|Immunogen||Recombinant protein corresponding to residues near the central region of mouse FoxP3 (UniProt Id: Q99JB6)|
Reacts with: mouse (Q99JB6).
Weaker signal: rat.
No signal: human (Q9BZS1).
Other species not tested yet.
IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical staining.
The transcription factor FoxP3 belongs to the family of forkhead transcription factors and controls the differentiation and function of regulatory T-cells (Tregs) (1). Tregs develop either in the thymus (tTreg), where they represent only ~2-3% of developing CD4+ thymocytes, or in the periphery by conversion of conventional CD4+ T cells into peripherally induced Treg (pTreg) cells (2). Expression of FoxP3 is induced by strong and persistent T cell receptor (TCR) signaling. FoxP3 binds DNA as homodimer or heterodimer with Foxp1 and interacts with other downstream transcription factors of TCR signaling, e.g., AP-1 transcription factors, NF-κB or Runx1 (1). Increasing evidence suggests that FoxP3 is expressed not only in Tregs, but also in a variety of tumor cells. However, tumor-FoxP3 has an inconsistent functional role and acts either as a tumor-suppressor or as a tumor-promoting factor (3). Mutations in FoxP3 lead to the severe autoimmune phenomena observed in patients with immune dysregulation, polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) and in scurfy mice (4).