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T-bet antibody mouse specific - HS-487 017

T-bet is a transcription factor expressed by several lymphocyte lineages
Rat monoclonal purified IgG
Cat. No.: HS-487 017
Amount: 100 µg
Price: $415.00
Cat. No. HS-487 017 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
IHC-P: 1 : 100 (see remarks) gallery  
Clone SY-87E12
Subtype IgG2a (κ light chain)
Immunogen Synthetic peptide corresponding to residues surrounding AA 470 of mouse T-bet (UniProt Id: Q9JKD8)
Reactivity Reacts with: mouse (Q9JKD8).
No signal: human.
Other species not tested yet.

IHC-P: Antigen retrieval in pressure cooker is recommended. The antibody shows some unspecific staining in mouse cerebellum.

Data sheet hs-487_017.pdf
Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.
Cat. No.: HS-487 017
Amount: 100 µg
Price: $415.00

T-bet (also known as Tbx21) belongs to the T-box subfamily Tbr1, and is an important transcription factor for the immune system. T-bet is a potent transactivator of the IFNγ gene and its expression correlates with IFNγ expression in Th1 cells, NK cells, and B cells (1). In CD4 T cells, Th1 and Th2 lineage commitment is regulated by opposing actions of T-bet and GATA-3 (2). T-bet promotes CD4 Th1 differentiation and represses alternative cell fates like Th2, or Th17, whereas GATA-3 is the lineage determining transcription factor for Th2 cells (2). CD8+ T cells require T-bet expression to differentiate into effector cells (3). In NK cells, T-bet expression is required for NK cell maturation (4).