T-bet antibody mouse specific - HS-487 008

T-bet is a transcription factor expressed by several lymphocyte lineages

Rabbit monoclonal recombinant IgG

Cat. No.: HS-487 008

Amount: 50 µg

Price: $415.00

Cat. No. HS-487 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: 1 : 200 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb87E12
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues surrounding AA 470 of mouse T-bet (UniProt Id: Q9JKD8)
Reactivity	Reacts with: mouse (Q9JKD8). No signal: human (Q9UL17), rat. Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal rat antibody clone SY-87E12. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. IHC-P: Heat-mediated antigen retrieval (Tris/EDTA pH 9.0) is recommended for immunohistochemical staining. The antibody shows some unspecific staining in mouse cerebellum.
Data sheet	hs-487_008.pdf

Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.

Cat. No.: HS-487 008

Amount: 50 µg

Price: $415.00

IHC-P

Background

T-bet (also known as Tbx21) belongs to the T-box subfamily Tbr1, and is an important transcription factor for the immune system. T-bet is a potent transactivator of the IFNγ gene and its expression correlates with IFNγ expression in Th1 cells, NK cells, and B cells (1). In CD4 T cells, Th1 and Th2 lineage commitment is regulated by opposing actions of T-bet and GATA-3 (2). T-bet promotes CD4 Th1 differentiation and represses alternative cell fates like Th2, or Th17, whereas GATA-3 is the lineage determining transcription factor for Th2 cells (2). CD8+ T cells require T-bet expression to differentiate into effector cells (3). In NK cells, T-bet expression is required for NK cell maturation (4).

1 ) Szabo SJ et al., Cell (2000)
2 ) Jenner RG et al., Proc Natl Acad Sci U S A (2009)
3 ) Sullivan BM et al., Proc Natl Acad Sci U S A (2003)
4 ) Huang C et al., Front Immunol (2021)

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