Tailor-made Antibodies
and Tools for Life Science

T-bet antibody mouse specific - HS-487 008

T-bet is a transcription factor expressed by several lymphocyte lineages
Rabbit monoclonal recombinant IgG
Cat. No.: HS-487 008
Amount: 50 µg
Price: $420.00
Cat. No. HS-487 008 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: 1 : 200 (see remarks) gallery  
Clone Rb87E12
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues surrounding AA 470 of mouse T-bet (UniProt Id: Q9JKD8)
Reactivity Reacts with: mouse (Q9JKD8).
No signal: human (Q9UL17), rat.
Other species not tested yet.
Remarks

This antibody is a chimeric antibody based on the monoclonal rat antibody clone SY-87E12. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IHC-P: Heat-mediated antigen retrieval (Tris/EDTA pH 9.0) is recommended for immunohistochemical staining. The antibody shows some unspecific staining in mouse cerebellum.

Data sheet hs-487_008.pdf
Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.
Cat. No.: HS-487 008
Amount: 50 µg
Price: $420.00