Synaptic Systems

Cat. No.: HS-484 017

Amount: 100 µg

Price: $420.00

WB

IHC-P

Background

Inducible nitric oxide synthase (iNOS) belongs together with endothelial NOS (eNOS) and neuronal NOS (nNOS) to the NOS family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. In contrast to the constitutively expressed, calcium-dependent eNOS and nNOS isoforms, iNOS is calcium-independent and usually activated in defense responses such as infection and inflammation (1). Expression of the iNOS gene is induced in vitro in macrophages polarized to the pro-inflammatory M1 state (2). NO-producing tumoricidal macrophages in the tumor microenvironment can modulate immune responses and promote apoptosis of tumor cells (2). In the brain, iNOS is normally not expressed except in chronic and acute inflammatory conditions. In ischemic brain injury, iNOS was found to be highly expressed in neutrophils and endothelium (3) and in M1-like pro-inflammatory microglia (4).

1 ) Tengan CH et al., Biochim Biophys Acta Bioenerg (2017)
2 ) Kashfi K et al., Cells (2021)
3 ) Garcia-Bonilla L et al., J Immunol (2014)
4 ) Collmann FM et al., Front Immunol (2019)

Cat. No. HS-484 017	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: 1 : 200 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	SY-171D8E8
Subtype	IgG2b (λ light chain)
Immunogen	synthetic peptide corresponding to residues near the carboxy terminus of mouse iNOS (UniProt Id: P29477)
Reactivity	Reacts with: mouse (P29477). No signal: rat, human. Other species not tested yet.
Data sheet	hs-484_017.pdf

iNOS antibody mouse specific - HS-484 017