Synaptic Systems

Cat. No.: HS-466 003

Amount: 50 µg

Price: $370.00

WB

IHC

IHC-P

Background

CD86 (Cluster of Differentiation 86, also known as B7.2) belongs to the B7 family of immune-regulatory cell-surface protein ligands (1). CD86 and the genetically closely linked CD80 protein (also known as B7.1) are expressed by antigen presenting cells and provide costimulatory signals necessary for T cell activation and tolerance via interaction with CD28 and cytotoxic T-lymphocyte antigen 4 (CTLA-4) expressed on T-cells. However, CD80 and CD86 have non-equivalent roles in immune modulation: CD86 is the dominant ligand for proliferation and survival of regulatory T cells (Tregs) (2) and shows in comparison with CD80 very high efficiency at increasing T cell killing capacity (3). CD86 is expressed only at low levels on resting B cells, dendritic cells and macrophages; activation results in enhanced CD86 expression (Collins et al., 2005). In the CNS, CD86 upregulation is a marker of activated pro-inflammatory M1 microglia (4). In oncology research, CD86 is a biomarker to phenotypically differentiate classically activated M1 macrophages from alternatively activated M2 macrophages in the tumor microenvironment (5).

1 ) Collins M et al., Genome Biol (2005)
2 ) Halliday N et al., Front Immunol (2020)
3 ) Thiel M et al., Immunology (2010)
4 ) Jurga AM et al., Front Cell Neurosci (2020)
5 ) Li C et al., J Immunother Cancer (2021)

Cat. No. HS-466 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 250 gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 (see remarks) gallery IHC-P: 1 : 400 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 291 to 309 from mouse Cd86 (UniProt Id: P42082)
Reactivity	Reacts with: mouse (P42082). No signal: human (P42081), rat. Other species not tested yet.
Remarks	IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical staining of PFA fixed tissues. Methanol fixation is required for immunohistochemical staining of fresh frozen tissues.
Data sheet	hs-466_003.pdf

CD86 antibody mouse specific - HS-466 003