Synaptic Systems

Cat. No.: HS-460 008

Amount: 100 µl

Price: $415.00

WB

IHC-P

Background

CD68, also called Lamp-4 in humans or macrosialin in mice, is a highly glycosylated type I transmembrane protein that belongs to the lysosome associated membrane protein (LAMP) family (1). CD68 expression is increased in cells associated with elevated phagocytic and degradative activity. High CD68 expression is detected in cells of the mononuclear phagocyte lineage including macrophages, osteoclasts, and myeloid dendritic cells (2). CD68 is a marker of activated microglia and only expressed at low levels in resting microglia (3). Staining for CD68 is predominantly intracellular, only 10 -15% of it is found on the cell surface. In oncology research, CD68 is the major biomarker for quantification of tumor-associated macrophages (TAMs) (4). High infiltration of CD68+ macrophages is an independent prognostic factor for overall survival in several tumor entities (5).

1 ) Holness CL et al., J Biol Chem (1993)
2 ) Chistiakov DA et al., Lab Invest (2017)
3 ) Hendrickx DAE et al., J Neuroimmunol (2017)
4 ) Larionova I et al., Front Oncol (2020)
5 ) Yu M et al., Cancer Manag Res (2019)

Other products for this target

Cat. No. HS-460 008	100 µl purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb186F9
Subtype	IgG1 (κ light chain)
Immunogen	synthetic peptide corresponding to residues near the carboxy terminus of human CD68 (UniProt Id: P34810)
Reactivity	Reacts with: human (P34810). No signal: mouse (P31996). Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal rat antibody clone 186F9B4. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	hs-460_008.pdf

CD68 antibody human specific - HS-460 008