Nucleocapsid CoV-2 antibody - HS-452 011BT

CoV2 protein N packages the viral genome RNA

Mouse monoclonal purified IgG

Cat. No.: HS-452 011BT

Amount: 100 µg

Price: $465.00

Cat. No. HS-452 011BT	100 µg purified IgG, lyophilized, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: yes gallery ELISA: (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Label	biotin
Clone	4A8
Subtype	IgG2b (κ light chain)
Specificity	Specific for nucleocapsid from SARS-CoV2
Remarks	ELISA: For a higher sensitivity in sandwich ELISA we recommend the use of cat. no. HS-452 011 as capture antibody and HS-cat. no. 452 111BT as detector antibody
Data sheet	hs-452_011bt.pdf

Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.

Cat. No.: HS-452 011BT

Amount: 100 µg

Price: $465.00

IHC-P

ELISA

Background

The severe acute respiratory syndrome coronavirus type 2 (SARS-CoV2) is an enveloped positive-sense single stranded RNA virus that has been identified in the beginning of 2020 (1). It infects human host cells by docking via its spike protein (S) to the ACE2 surface receptor (2) and can cause mild to very severe and even deadly Covid-19 courses (3).
The very abundant Nucleocapsid or N-protein packages the viral RNA (4) and shares only little homology to other abundant members of the coronavirus family like NL63, 229E, HKU1 or OC43. This characteristic makes it a suitable target to discriminate between Covid-19 and other coronavirus infections.

1 ) et al., Nat Microbiol (2020)
2 ) Hoffmann M et al., Cell (2020)
3 ) Chen N et al., Lancet (2020)
4 ) Chang CK et al., Antiviral Res (2014)

Other products for this target