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CD45 antibody - HS-427 003

CD45 is a large abundant leukocyte-specific cell surface glycoprotein
Rabbit polyclonal purified antibody
Cat. No.: HS-427 003
Amount: 50 µg
Price: $370.00
Cat. No. HS-427 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 (AP-staining) gallery  
ICC: 1 : 500 gallery  
IHC-P: 1 : 1000 gallery  
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of human CD45 (UniProt Id: P08575)
Reactivity Reacts with: human (P08575).
No signal: mouse (P06800-1), rat.
Other species not tested yet.
Specificity The antibody recognizes all human CD45 isoforms.
Data sheet hs-427_003.pdf
Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.
Cat. No.: HS-427 003
Amount: 50 µg
Price: $370.00
Background

CD45, also designated as Leukocyte Common Antigen (LCA) or Protein tyrosine phosphatase receptor type C (PTPRC), is a type I transmembrane protein expressed on all nucleated cells of the hematopoietic system, except mature erythrocytes and platelets (1). Several isoforms of CD45 have been identified which are generated by differential splicing of exons 4, 5, and 6, thereby generating the CD45RA, RB and RC isoforms, respectively (2). Expression of the isoforms differs according to cell type and functional status. The CD45RABC (B220) long isoform is almost exclusively expressed on B cells. (3). Naïve human T cells express the high molecular weight isoform CD45RA. Activation of T cells and differentiation to memory T cells is accompanied by exon exclusion for production of the short isoform CD45RO (3). CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. Thus, CD45-deficient humans and mice develop a severe-combined immunodeficiency (SCID) phenotype (4). In macrophages, CD45 plays a central role in their adhesion, morphology and motility (5).

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